TY - JOUR T1 - CRISPR/Cas9-induced disruption of Bodo saltans paraflagellar rod-2 gene reveals its importance for cell survival JF - Environmental Microbiology Y1 - 2022 A1 - Gomaa, Fatma A1 - Li, Zhu-Hong A1 - Beaudoin, David J A1 - Alzan, Heba A1 - Girguis, Peter R. A1 - Docampo, Roberto A1 - Edgcomb, Virginia P PB - John Wiley & Sons, Inc. Hoboken, USA ER - TY - JOUR T1 - Evidence for Horizontal and Vertical Transmission of Mtr-Mediated Extracellular Electron Transfer among the Bacteria JF - mBio Y1 - 2022 A1 - Baker, Isabel R A1 - Conley, Bridget E A1 - Gralnick, Jeffrey A A1 - Girguis, Peter R. PB - American Society for Microbiology 1752 N St., NW, Washington, DC VL - 13 IS - 1 ER - TY - JOUR T1 - Novel insights on obligate symbiont lifestyle and adaptation to chemosynthetic environment as revealed by the giant tubeworm genome JF - Molecular biology and evolution Y1 - 2022 A1 - de Oliveira, André Luiz A1 - Mitchell, Jessica A1 - Peter Girguis A1 - Bright, Monika PB - Oxford University Press VL - 39 IS - 1 ER - TY - JOUR T1 - Carbonate-hosted microbial communities are prolific and pervasive methane oxidizers at geologically diverse marine methane seep sites JF - Proceedings of the National Academy of Sciences Y1 - 2021 A1 - Marlow, Jeffrey J A1 - Hoer, Daniel A1 - Jungbluth, Sean P A1 - Reynard, Linda M A1 - Gartman, Amy A1 - Chavez, Marko S A1 - El-Naggar, Mohamed Y A1 - Tuross, Noreen A1 - Orphan, Victoria J A1 - Girguis, Peter R. PB - National Academy of Sciences VL - 118 IS - 25 ER - TY - JOUR T1 - Cooccurring Activities of Two Autotrophic Pathways in Symbionts of the Hydrothermal Vent Tubeworm Riftia pachyptila JF - Applied and Environmental Microbiology Y1 - 2021 A1 - Leonard, Juliana M A1 - Mitchell, Jessica A1 - Beinart, Roxanne A A1 - Delaney, Jennifer A A1 - Sanders, Jon G A1 - Ellis, Greg A1 - Goddard, Ethan A A1 - Girguis, Peter R. A1 - Scott, Kathleen M PB - American Society for Microbiology 1752 N St., NW, Washington, DC VL - 87 IS - 17 ER - TY - JOUR T1 - The grayness of the origin of life JF - Life Y1 - 2021 A1 - Smith, Hillary H A1 - Hyde, Andrew S A1 - Simkus, Danielle N A1 - Libby, Eric A1 - Maurer, Sarah E A1 - Graham, Heather V A1 - Kempes, Christopher P A1 - Sherwood Lollar, Barbara A1 - Chou, Luoth A1 - Ellington, Andrew D A1 - others PB - Multidisciplinary Digital Publishing Institute VL - 11 IS - 6 ER - TY - JOUR T1 - Interactions Between Iron Sulfide Minerals and Organic Carbon: Implications for Biosignature Preservation and Detection JF - Astrobiology Y1 - 2021 A1 - Picard, Aude A1 - Gartman, Amy A1 - Girguis, Peter R. PB - Mary Ann Liebert, Inc., publishers 140 Huguenot Street, 3rd Floor New … VL - 21 IS - 5 ER - TY - JOUR T1 - Multiple carbon incorporation strategies support microbial survival in cold subseafloor crustal fluids JF - Science Advances Y1 - 2021 A1 - Trembath-Reichert, Elizabeth A1 - Shah Walter, Sunita R A1 - Ortiz, Marc Alec Fontánez A1 - Carter, Patrick D A1 - Girguis, Peter R. A1 - Huber, Julie A PB - American Association for the Advancement of Science VL - 7 IS - 18 ER - TY - JOUR T1 - Protect high seas biodiversity JF - Science Y1 - 2021 A1 - Helm, Rebecca R A1 - Clark, Nichola A1 - Harden-Davies, Harriet A1 - Amon, Diva A1 - Peter Girguis A1 - Bordehore, Cesar A1 - Earle, Sylvia A1 - Gibbons, Mark J A1 - Golbuu, Yimnang A1 - Haddock, Steven H. D. A1 - others PB - American Association for the Advancement of Science VL - 372 IS - 6546 ER - TY - JOUR T1 - Spatially resolved correlative microscopy and microbial identification reveal dynamic depth-and mineral-dependent anabolic activity in salt marsh sediment JF - Environmental microbiology Y1 - 2021 A1 - Marlow, Jeffrey A1 - Spietz, Rachel A1 - Kim, Keun-Young A1 - Ellisman, Mark A1 - Peter Girguis A1 - Hatzenpichler, Roland PB - John Wiley & Sons, Inc. Hoboken, USA VL - 23 IS - 8 ER - TY - JOUR T1 - Sulfur bacteria promote dissolution of authigenic carbonates at marine methane seeps JF - The ISME journal Y1 - 2021 A1 - Leprich, Dalton J A1 - Flood, Beverly E A1 - Schroedl, Peter R A1 - Ricci, Elizabeth A1 - Marlow, Jeffery J A1 - Girguis, Peter R. A1 - Bailey, Jake V PB - Nature Publishing Group VL - 15 IS - 7 ER - TY - JOUR T1 - Towards a more universal life detection strategy JF - Bulletin of the American Astronomical Society Y1 - 2021 A1 - Chou, Luoth A1 - Grefenstette, Natalie A1 - Johnson, Sarah S A1 - Graham, Heather A1 - Mahaffy, Paul A1 - Kempes, Christopher A1 - Elsila, Jamie E A1 - Libby, Eric A1 - Ellington, Andrew A1 - Anslyn, Eric A1 - others PB - American Astronomical Society VL - 53 IS - 4 ER - TY - JOUR T1 - Fundamentals of benthic microbial fuel cells: theory, development and application Y1 - 2020 A1 - Girguis, Peter R. A1 - Nielsen, Mark A1 - Reimers, Clare PB - Springer Verlag Press ER - TY - CONF T1 - Agnostic approaches to extant life detection on Ocean Worlds T2 - Ocean Sciences Meeting 2020 Y1 - 2020 A1 - Graham, Heather A1 - Johnson, Sarah A1 - Anslyn, Eric A1 - Conrad, Pamela A1 - Cronin, Leroy A1 - Ellington, Andrew A1 - Cook, Jamie Elsila A1 - Girguis, Peter R. A1 - House, Christopher H A1 - Kempes, Chris A1 - others JF - Ocean Sciences Meeting 2020 PB - AGU ER - TY - JOUR T1 - Author Correction: A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge JF - Scientific Reports Y1 - 2020 A1 - Meyer, Julie L A1 - Jaekel, Ulrike A1 - Tully, Benjamin J A1 - Glazer, Brian T A1 - Wheat, C Geoffrey A1 - Lin, Huei-Ting A1 - Hsieh, Chih-Chiang A1 - Cowen, James P A1 - Hulme, Samuel M A1 - Girguis, Peter R. A1 - others PB - Nature Publishing Group VL - 10 IS - 1 ER - TY - JOUR T1 - A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge (vol 6, 22541, 2016) JF - SCIENTIFIC REPORTS Y1 - 2020 A1 - Meyer, Julie L A1 - Jaekel, Ulrike A1 - Tully, Benjamin J A1 - Glazer, Brian T A1 - Wheat, C Geoffrey A1 - Lin, Huei-Ting A1 - Hsieh, Chih-Chiang A1 - Cowen, James P A1 - Hulme, Samuel M A1 - Girguis, Peter R. A1 - others PB - NATURE PUBLISHING GROUP MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND VL - 10 IS - 1 ER - TY - CONF T1 - From the Seafloor to the Surface: In situ Chemical Analysis of Rising Bubbles along the Cascadia Margin T2 - Ocean Sciences Meeting 2020 Y1 - 2020 A1 - Michel, Anna A1 - Johnson, Andrew Stafford A1 - Fauria, Kristen A1 - Preston, Victoria A1 - Nicholson, David P A1 - Hoer, Daniel A1 - Girguis, Peter R. A1 - Wankel, Scott D JF - Ocean Sciences Meeting 2020 PB - AGU ER - TY - JOUR T1 - Fundamentals of benthic microbial fuel cells: theory, development and application Y1 - 2020 A1 - Girguis, Peter R. A1 - Nielsen, Mark A1 - Reimers, Clare PB - Springer Verlag Press ER - TY - JOUR T1 - Genetic tool development in marine protists: emerging model organisms for experimental cell biology JF - Nature methods Y1 - 2020 A1 - Faktorová, Drahom{\'ıra A1 - Nisbet, R Ellen R A1 - Fernández Robledo, José A A1 - Casacuberta, Elena A1 - Sudek, Lisa A1 - Allen, Andrew E A1 - Ares, Manuel A1 - Aresté, Cristina A1 - Balestreri, Cecilia A1 - Barbrook, Adrian C A1 - others PB - Nature Publishing Group VL - 17 IS - 5 ER - TY - JOUR T1 - Mud, microbes, and macrofauna: seasonal dynamics of the iron biogeochemical cycle in an intertidal mudflat JF - Frontiers in Marine Science Y1 - 2020 A1 - Beam, Jacob P A1 - George, Sarabeth A1 - Record, Nicholas R A1 - Countway, Peter D A1 - Johnston, David T A1 - Girguis, Peter R. A1 - David Emerson PB - Frontiers ER - TY - JOUR T1 - Physiological dynamics of chemosynthetic symbionts in hydrothermal vent snails JF - The ISME journal Y1 - 2020 A1 - Breusing, Corinna A1 - Mitchell, Jessica A1 - Delaney, Jennifer A1 - Sylva, Sean P A1 - Seewald, Jeffrey S A1 - Girguis, Peter R. A1 - Beinart, Roxanne A PB - Nature Publishing Group VL - 14 IS - 10 ER - TY - JOUR T1 - Roadmap for naming uncultivated Archaea and Bacteria JF - Nature microbiology Y1 - 2020 A1 - Murray, Alison E A1 - Freudenstein, John A1 - Gribaldo, Simonetta A1 - Hatzenpichler, Roland A1 - Hugenholtz, Philip A1 - Kämpfer, Peter A1 - Konstantinidis, Konstantinos T A1 - Lane, Christopher E A1 - Papke, R Thane A1 - Parks, Donovan H A1 - others PB - Nature Publishing Group VL - 5 IS - 8 ER - TY - CONF T1 - Role of Pressure in the Formation of Organo-Mineral Interactions: Implications for Organic Preservation in the Anoxic Subsurface T2 - AGU Fall Meeting Abstracts Y1 - 2020 A1 - Picard, Aude A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2020 ER - TY - JOUR T1 - Undersea Supercharger™ Network?: A Commentary Promoting In-Situ Methane to Fuel Expansion of Seafloor Robotics JF - Marine Technology Society Journal Y1 - 2020 A1 - Waterston, John A1 - Florea, Rachel A1 - Peter Girguis PB - Marine Technology Society VL - 54 IS - 5 ER - TY - JOUR T1 - Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens JF - PloS one Y1 - 2020 A1 - Totoiu, Christian A A1 - Phillips, Jessica M A1 - Reese, Aspen T A1 - Majumdar, Sudipta A1 - Girguis, Peter R. A1 - Raston, Colin L A1 - Gregory A. Weiss PB - Public Library of Science San Francisco, CA USA VL - 15 IS - 1 ER - TY - JOUR T1 - Authigenic metastable iron sulfide minerals preserve microbial organic carbon in anoxic environments JF - Chemical Geology Y1 - 2019 A1 - Picard, Aude A1 - Gartman, Amy A1 - Cosmidis, Julie A1 - Obst, Martin A1 - Vidoudez, Charles A1 - Clarke, David R A1 - Girguis, Peter R. PB - Elsevier VL - 530 ER - TY - JOUR T1 - The bacterial symbionts of closely related hydrothermal vent snails with distinct geochemical habitats show broad similarity in chemoautotrophic gene content JF - Frontiers in microbiology Y1 - 2019 A1 - Beinart, Roxanne A A1 - Luo, Chengwei A1 - Konstantinidis, Konstantinos T A1 - Stewart, Frank J A1 - Girguis, Peter R. PB - Frontiers ER - TY - CONF T1 - Exploring ocean worlds: a systems-level approach for the search for life beyond Earth T2 - 2019 Astrobiology Science Conference Y1 - 2019 A1 - German, Christopher R A1 - Blackman, Donna K A1 - Fisher, Andrew T A1 - Girguis, Peter R. A1 - Hand, Kevin P A1 - Hoehler, Tori M A1 - Huber, Julie A A1 - Marshall, John C A1 - Seewald, Jeffrey A1 - Shock, Everett A1 - others JF - 2019 Astrobiology Science Conference PB - AGU ER - TY - JOUR T1 - Hydrogen does not appear to be a major electron donor for symbiosis with the deep-sea hydrothermal vent tubeworm Riftia pachyptila JF - Applied and environmental microbiology Y1 - 2019 A1 - Mitchell, Jessica H A1 - Leonard, Juliana M A1 - Delaney, Jennifer A1 - Girguis, Peter R. A1 - Scott, Kathleen M PB - American Society for Microbiology 1752 N St., NW, Washington, DC VL - 86 IS - 1 ER - TY - JOUR T1 - Hydrothermal energy transfer and organic carbon production at the deep seafloor JF - Frontiers in Marine Science Y1 - 2019 A1 - Lebris, Nadine A1 - Yücel, Mustafa A1 - Das, Anindita A1 - Sievert, Stefan M A1 - LokaBharathi, PonnaPakkam A1 - Girguis, Peter R. PB - Frontiers VL - 5 ER - TY - CONF T1 - In situ Chemical Analysis of Rising Bubbles Along the Cascadia Margin T2 - AGU Fall Meeting Abstracts Y1 - 2019 A1 - Wankel, Scott D A1 - Johnson, Andrew S A1 - Hoer, Daniel A1 - Girguis, Peter R. A1 - Michel, AP JF - AGU Fall Meeting Abstracts VL - 2019 ER - TY - CONF T1 - The Laboratory for Agnostic Biosignatures Project T2 - 2019 Astrobiology Science Conference Y1 - 2019 A1 - Johnson, Sarah A1 - Graham, Heather A1 - Anslyn, Eric A1 - Conrad, Pamela A1 - Cronin, Leroy A1 - Ellington, Andrew A1 - Elsila, Jamie A1 - Girguis, Peter R. A1 - House, Christopher H A1 - Kempes, Chris A1 - others JF - 2019 Astrobiology Science Conference PB - AGU ER - TY - CONF T1 - Methane Hydrates as an Astrobiological Target: Lessons from Seafloor Methane Seeps T2 - 2019 Astrobiology Science Conference Y1 - 2019 A1 - Marlow, Jeffrey J A1 - Hoer, Daniel A1 - Reynard, Linda A1 - Girguis, Peter R. JF - 2019 Astrobiology Science Conference PB - AGU ER - TY - JOUR T1 - Methane-linked mechanisms of electron uptake from cathodes by Methanosarcina barkeri JF - MBio Y1 - 2019 A1 - Rowe, Annette R A1 - Xu, Shuai A1 - Gardel, Emily A1 - Bose, Arpita A1 - Peter Girguis A1 - Amend, Jan P A1 - El-Naggar, Mohamed Y PB - American Society for Microbiology 1752 N St., NW, Washington, DC VL - 10 IS - 2 ER - TY - CONF T1 - A peak behind the curtain: What Earth's thin biosphere reveals about the deep subsurface. T2 - AGU Fall Meeting Abstracts Y1 - 2019 A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2019 ER - TY - JOUR T1 - Remarkable capacity for anaerobic oxidation of methane at high methane concentration JF - Geophysical Research Letters Y1 - 2019 A1 - Bowles, MW A1 - Samarkin, V. A. A1 - Hunter, K. S. A1 - Finke, N A1 - Teske, AP A1 - Girguis, P. R. A1 - Joye, S. B. VL - 46 IS - 21 ER - TY - JOUR T1 - Synergistic substrate cofeeding stimulates reductive metabolism JF - Nature Metabolism Y1 - 2019 A1 - Park, Junyoung O A1 - Liu, Nian A1 - Holinski, Kara M A1 - Emerson, David F A1 - Qiao, Kangjian A1 - Woolston, Benjamin M A1 - Xu, Jingyang A1 - Lazar, Zbigniew A1 - Islam, M Ahsanul A1 - Vidoudez, Charles A1 - others PB - Nature Publishing Group VL - 1 IS - 6 ER - TY - CONF T1 - Water, Water Everywhere: Bioelectrochemical Processes at Some of Earth’s most Extreme Aquatic Environments, and Implications for Astrobiological Research T2 - 2019 Astrobiology Science Conference Y1 - 2019 A1 - Girguis, Peter R. A1 - Reimers, Clare E A1 - Gulay, Arda A1 - Baker, Isabel JF - 2019 Astrobiology Science Conference PB - AGU ER - TY - CONF T1 - 200,000 KB Under The Sea: Combining High-speed Underwater Communications and Biogeochemical Sensors/Samplers for Deep-Sea Autonomous Exploration T2 - AGU Fall Meeting Abstracts Y1 - 2018 A1 - Girguis, Peter R. A1 - Hoer, Daniel A1 - Farr, Norman A1 - Wankel, Scott D A1 - Michel, Anna JF - AGU Fall Meeting Abstracts VL - 2018 ER - TY - JOUR T1 - Advancing ocean science and exploration through telepresence JF - Deep Sea Research Part II: Topical Studies in Oceanography Y1 - 2018 A1 - Raineault, Nicole A A1 - Bell, Katherine LC A1 - Peter Girguis VL - 150 ER - TY - CONF T1 - Development and deployment of the autonomous biogeochemical in-situ sensing system (ABISS) T2 - 2018 Ocean Sciences Meeting Y1 - 2018 A1 - Girguis, Peter R. A1 - Michel, Anna A1 - Wankel, Scott D A1 - Farr, Norman A1 - Hoer, Daniel A1 - Pontbriand, Clifford JF - 2018 Ocean Sciences Meeting PB - AGU ER - TY - JOUR T1 - Exploration of the northern guaymas basin JF - Oceanography Y1 - 2018 A1 - Soule, S Adam A1 - Seewald, Jeff A1 - Wankel, Scott A1 - Michel, Anna A1 - Beinart, Roxanne A1 - Briones, Elva Escobar A1 - Dominguez, Esmerelda Morales A1 - Peter Girguis A1 - Coleman, Dwight A1 - Raineault, Nicole A A1 - others PB - OCEANOGRAPHY SOC PO BOX 1931, ROCKVILLE, MD USA VL - 31 IS - 1 ER - TY - CONF T1 - First-time combination of underwater sensors for carbon biogeochemistry in deep-sea environments T2 - 2018 Ocean Sciences Meeting Y1 - 2018 A1 - Wankel, Scott D A1 - Michel, Anna A1 - Hoer, Daniel A1 - Girguis, Peter R. JF - 2018 Ocean Sciences Meeting PB - AGU ER - TY - JOUR T1 - Harnessing a methane-fueled, sediment-free mixed microbial community for utilization of distributed sources of natural gas JF - Biotechnology and bioengineering Y1 - 2018 A1 - Marlow, Jeffrey J A1 - Kumar, Amit A1 - Enalls, Brandon C A1 - Reynard, Linda M A1 - Tuross, Noreen A1 - Stephanopoulos, Gregory A1 - Peter Girguis VL - 115 IS - 6 ER - TY - JOUR T1 - Hydrothermal energy transfer and organic carbon production at the deep seafloor JF - Front Microbiol Y1 - 2018 A1 - Nadine, LB A1 - Yucel, M. A1 - Das, A A1 - Sievert, S. M. A1 - Girguis, P. R. VL - 5 ER - TY - JOUR T1 - In situ carbon isotopic exploration of an active submarine volcano JF - Deep Sea Research Part II: Topical Studies in Oceanography Y1 - 2018 A1 - Michel, Anna PM A1 - Wankel, Scott D A1 - Kapit, Jason A1 - Sandwith, Zoe A1 - Girguis, Peter R. PB - Pergamon VL - 150 ER - TY - JOUR T1 - Linking Metabolic Activity, Microbial Identity, and Microscale Spatial Arrangements in Chemosynthetic Seafloor Habitats JF - Ocean Worlds Y1 - 2018 A1 - Marlow, J A1 - Hatzenpichler, R A1 - Girguis, P. VL - 2085 ER - TY - JOUR T1 - Microbial decomposition of marine dissolved organic matter in cool oceanic crust JF - Nature Geoscience Y1 - 2018 A1 - Shah Walter, Sunita R A1 - Jaekel, Ulrike A1 - Osterholz, Helena A1 - Fisher, Andrew T A1 - Huber, Julie A A1 - Pearson, Ann A1 - Dittmar, Thorsten A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 11 IS - 5 ER - TY - CONF T1 - Microbial Preference for Dissolved Organic Matter that is 14 C-enriched, 13 C-enriched and Molecularly Distinct in the North Pond Subsurface T2 - AGU Fall Meeting Abstracts Y1 - 2018 A1 - Shah Walter, SR A1 - Osterholz, Helena A1 - Dittmar, Thorsten A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2018 ER - TY - CONF T1 - New Insights into Oceanic Spreading Centers from Seafloor Observatories Posters T2 - AGU Fall Meeting 2018 Y1 - 2018 A1 - Baillard, Christian A1 - Barreyre, Thibaut A1 - Matabos, Marjolaine A1 - Butterfield, David A A1 - Reysenbach, Anna-Louise A1 - Girguis, Peter R. JF - AGU Fall Meeting 2018 PB - AGU ER - TY - JOUR T1 - Nitrogen cycling of active bacteria within oligotrophic sediment of the Mid-Atlantic Ridge flank JF - Geomicrobiology Journal Y1 - 2018 A1 - Reese, Brandi Kiel A1 - Zinke, Laura A A1 - Sobol, Morgan S A1 - LaRowe, Doug E A1 - Orcutt, Beth N A1 - Zhang, Xinxu A1 - Jaekel, Ulrike A1 - Wang, Fengping A1 - Dittmar, Thorsten A1 - Defforey, Delphine A1 - others PB - Taylor & Francis VL - 35 IS - 6 ER - TY - JOUR T1 - Peering into the abyss: studying our own ocean to advance astrobiology JF - Oceanography Y1 - 2018 A1 - Girguis, Peter R. A1 - Fundis, Allison PB - OCEANOGRAPHY SOC PO BOX 1931, ROCKVILLE, MD USA VL - 31 IS - 1 ER - TY - CONF T1 - Rock-hosted microbial communities possess substantial methane oxidizing potential at geologically diverse methane seep sites T2 - 2018 Ocean Sciences Meeting Y1 - 2018 A1 - Marlow, Jeffrey J A1 - Gartman, Amy A1 - Jungbluth, Sean A1 - Hoer, Daniel A1 - Reynard, Linda A1 - Tuross, Noreen A1 - Orphan, Victoria J A1 - Girguis, Peter R. JF - 2018 Ocean Sciences Meeting PB - AGU ER - TY - CONF T1 - The seas we've hardly seen: Advancing our understanding of deep sea processes through advanced underwater and shipboard communications T2 - 2018 Ocean Sciences Meeting Y1 - 2018 A1 - Girguis, Peter R. A1 - Hoer, Daniel A1 - Beinart, Roxanne A1 - Pontbriand, Clifford A1 - Farr, Norman A1 - Panzarino, Jessica JF - 2018 Ocean Sciences Meeting PB - AGU ER - TY - JOUR T1 - Sequencing of bacteria or other species Y1 - 2018 A1 - Weitz, David A A1 - Zhang, Huidan A1 - CUI, Nai Wen A1 - Cai, Yamei A1 - Peter Girguis A1 - Stewart, Frank A1 - Sarode, Neha A1 - Kraft, Beate ER - TY - JOUR T1 - Somewhere, Beyond the Sea: Advancing Geochemical Sensor Technologies for Biological and Abiotic Analyses on Ocean Worlds JF - Ocean Worlds Y1 - 2018 A1 - Girguis, Peter R. A1 - Hoer, Daniel A1 - Michel, Anna A1 - Wankel, Scott D A1 - Baker, Izzy A1 - Farr, Norm VL - 2085 ER - TY - JOUR T1 - Sulfate-reducing bacteria influence the nucleation and growth of mackinawite and greigite JF - Geochimica et Cosmochimica Acta Y1 - 2018 A1 - Picard, Aude A1 - Gartman, Amy A1 - Clarke, David R A1 - Girguis, Peter R. PB - Pergamon VL - 220 ER - TY - JOUR T1 - Citation: Padilla, CC, Bristow LA, Sarode, N., Garcia-Robledo, E., Benson, CR JF - UNRECOGNIZED DIVERSITY OF MICROBES LINKING METHANOTROPHY TO NITROGEN LOSS IN MARINE OXYGEN MINIMUM ZONES Y1 - 2017 A1 - Bourbonnais, A A1 - Altabet, M.A. A1 - Girguis, P. R. A1 - Thamdrup, B A1 - Stewart, F. J. PB - Georgia Institute of Technology ER - TY - JOUR T1 - Co-registered geochemistry and metatranscriptomics reveal unexpected distributions of microbial activity within a hydrothermal vent field JF - Frontiers in microbiology Y1 - 2017 A1 - Olins, Heather C A1 - Rogers, Daniel R A1 - Preston, Christina A1 - Ussler III, William A1 - Pargett, Douglas A1 - Jensen, Scott A1 - Roman, Brent A1 - Birch, James M A1 - Scholin, Christopher A A1 - Haroon, M Fauzi A1 - others PB - Frontiers VL - 8 ER - TY - JOUR T1 - Environmental parameters of deep-sea hydrothermal vents from the Manus Basin Y1 - 2017 A1 - Meier, Dimitri V A1 - Pjevac, Petra A1 - Bach, Wolfgang A1 - Hourdez, Stéphane A1 - Girguis, Peter R. A1 - Vidoudez, Charles A1 - Amann, Rudolf A1 - Meyerdierks, Anke A1 - others ER - TY - JOUR T1 - Exploration of central california basins, cold seeps, and san juan seamount JF - Oceanography (Wash. DC) Y1 - 2017 A1 - Raineault, Nicole A1 - Peter Girguis A1 - Auscavitch, Steve A1 - Castillo, Chris A1 - Lubetkin, Megan A1 - Marlow, Jeffrey A1 - Kane, R VL - 30 ER - TY - JOUR T1 - Geochemically distinct carbon isotope distributions in Allochromatium vinosum DSM 180T grown photoautotrophically and photoheterotrophically JF - Geobiology Y1 - 2017 A1 - Tang, T A1 - Mohr, W A1 - Sattin, SR A1 - Rogers, D. R. A1 - Girguis, P. R. A1 - Pearson, A VL - 15 IS - 2 ER - TY - JOUR T1 - Iron sulfide formation on iron substrates by electrochemical reaction in anoxic conditions JF - Crystal Growth & Design Y1 - 2017 A1 - Guan, Xiaofei A1 - Enalls, Brandon C A1 - Clarke, David R A1 - Peter Girguis PB - American Chemical Society VL - 17 IS - 12 ER - TY - CONF T1 - Iron Sulfide Minerals Record Microbe-Mineral Interactions in Anoxic Environments T2 - AGU Fall Meeting Abstracts Y1 - 2017 A1 - Picard, Aude A1 - Gartman, Amy A1 - Cosmidis, Julie A1 - Clarke, David R A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2017 ER - TY - JOUR T1 - Microbes facilitate mineral deposition in bioelectrochemical systems JF - ACS Earth and Space Chemistry Y1 - 2017 A1 - Gartman, A. A1 - Picard, A A1 - Olins, H. C. A1 - Sarode, N A1 - Clarke, DR A1 - Girguis, P. R. PB - American Chemical Society VL - 1 IS - 5 ER - TY - JOUR T1 - Microbial response to oil enrichment in Gulf of Mexico sediment measured using a novel long-term benthic lander system JF - Elementa: Science of the Anthropocene Y1 - 2017 A1 - Orcutt, Beth N A1 - Lapham, Laura L A1 - Delaney, Jennifer A1 - Sarode, Neha A1 - Marshall, Kathleen S A1 - Whaley-Martin, Kelly J A1 - Slater, Greg A1 - Wheat, C Geoff A1 - Girguis, Peter R. A1 - Thomsen, Laurenz PB - University of California Press VL - 5 ER - TY - JOUR T1 - New Frontiers in Ocean Exploration The E/V Nautilus, NOAA Ship Okeanos Explorer, and R/V Falkor 2016 Field Season JF - Oceanography Y1 - 2017 A1 - Bell, Katherine LC A1 - Mowitt, William A1 - Zykov, Victor A1 - Delgado, James P A1 - Tartt, Mitchell A1 - Stout, Matthew A1 - Wagner, Katie A1 - Marquis, Sarah A1 - Bell, Katherine LC A1 - Phillips, Brennan A1 - others PB - OCEANOGRAPHY SOC PO BOX 1931, ROCKVILLE, MD USA VL - 30 IS - 1 ER - TY - JOUR T1 - Niche partitioning of diverse sulfur-oxidizing bacteria at hydrothermal vents JF - The ISME journal Y1 - 2017 A1 - Meier, Dimitri V A1 - Pjevac, Petra A1 - Bach, Wolfgang A1 - Hourdez, Stephane A1 - Girguis, Peter R. A1 - Vidoudez, Charles A1 - Amann, Rudolf A1 - Meyerdierks, Anke PB - Nature Publishing Group VL - 11 IS - 7 ER - TY - JOUR T1 - Opinion: Telepresence is a potentially transformative tool for field science JF - Proceedings of the National Academy of Sciences Y1 - 2017 A1 - Marlow, Jeffrey A1 - Borrelli, Chiara A1 - Jungbluth, Sean P A1 - Hoffman, Colleen A1 - Marlow, Jennifer A1 - Girguis, Peter R. A1 - others PB - National Academy of Sciences VL - 114 IS - 19 ER - TY - JOUR T1 - Proteome evolution of deep-sea hydrothermal vent alvinellid polychaetes supports the ancestry of thermophily and subsequent adaptation to cold in some lineages JF - Genome Biology and Evolution Y1 - 2017 A1 - Fontanillas, Eric A1 - Galzitskaya, Oxana V A1 - Lecompte, Odile A1 - Lobanov, Mikhail Y A1 - Tanguy, Arnaud A1 - Mary, Jean A1 - Girguis, Peter R. A1 - Hourdez, Stéphane A1 - Jollivet, Didier PB - Oxford University Press VL - 9 IS - 2 ER - TY - CONF T1 - There and back again: An oceanographer's approach to delving into-and returning from-the unknown. T2 - AGU Fall Meeting Abstracts Y1 - 2017 A1 - Girguis, Peter R. A1 - Hoer, Daniel A1 - Michel, Anna A1 - Wankel, Scott D A1 - Farr, Norman A1 - Pontbriand, Clifford A1 - Raineault, Nicole JF - AGU Fall Meeting Abstracts VL - 2017 ER - TY - JOUR T1 - Toward establishing model organisms for marine protists: Successful transfection protocols for Parabodo caudatus (Kinetoplastida: Excavata) JF - Environmental microbiology Y1 - 2017 A1 - Gomaa, Fatma A1 - Garcia, Paulo A A1 - Delaney, Jennifer A1 - Girguis, Peter R. A1 - Buie, Cullen R A1 - Edgcomb, Virginia P VL - 19 IS - 9 ER - TY - JOUR T1 - Activity and diversity of aerobic methanotrophs in a coastal marine oxygen minimum zone JF - American Geophysical Union Y1 - 2016 A1 - Padilla,Cory C A1 - Bristow,Laura A A1 - Sarode, Neha D A1 - Garcia-Robledo,Emilio A1 - Girguis, Peter R. A1 - Thamdrup,Bo A1 - Stewart, Frank J VL - 2016 ER - TY - JOUR T1 - Advancing an In situ Laser Spectrometer for Carbon Isotope Analyses in the Deep Ocean JF - American Geophysical Union Y1 - 2016 A1 - Michel, A A1 - Wankel, S. D. A1 - Kapit, J A1 - Girguis, P. R. VL - 2016 ER - TY - JOUR T1 - Arsenic concentrations and species in three hydrothermal vent worms, Ridgeia piscesae, Paralvinella sulficola and Paralvinella palmiformis JF - Deep Sea Research Part I: Oceanographic Research Papers Y1 - 2016 A1 - Maher, WA A1 - Duncan, Elliott A1 - Dilly, Geoffrey A1 - Foster, Simon A1 - Krikowa, Frank A1 - Lombi, E A1 - Scheckel, Kirk A1 - Peter Girguis PB - Pergamon VL - 116 ER - TY - CONF T1 - Closing in on the limits of life through open-access instrumentation. T2 - AGU Fall Meeting Abstracts Y1 - 2016 A1 - Girguis, Peter R. A1 - Hoer, Daniel JF - AGU Fall Meeting Abstracts VL - 2016 ER - TY - JOUR T1 - A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge JF - Scientific reports Y1 - 2016 A1 - Meyer, Julie L A1 - Jaekel, Ulrike A1 - Tully, Benjamin J A1 - Glazer, Brian T A1 - Wheat, C Geoffrey A1 - Lin, Huei-Ting A1 - Hsieh, Chih-Chiang A1 - Cowen, James P A1 - Hulme, Samuel M A1 - Girguis, Peter R. A1 - others PB - Nature Publishing Group VL - 6 IS - 1 ER - TY - CONF T1 - E/V Nautilus Mapping and ROV Dives Reveal Hundreds of Vents along the West Coast of the United States T2 - AGU Fall Meeting Abstracts Y1 - 2016 A1 - Kane, Renato A1 - Raineault, Nicole A1 - Embley, Robert W A1 - Merle, Susan G A1 - Girguis, Peter R. A1 - Irish, Onni A1 - Lubetkin, Megan A1 - German, Christopher R A1 - Levin, Lisa A A1 - Cormier, Marie-Helene A1 - others JF - AGU Fall Meeting Abstracts VL - 2016 ER - TY - ABST T1 - Exploration and discovery of methane seeps and associated communities in the California Borderland Y1 - 2016 A1 - Levin, Lisa A1 - Girguis, Peter R. A1 - German, Christopher R A1 - Brennan, Michael L A1 - Tuzun, Suna A1 - Wagner, Jamie A1 - Smart, Clara A1 - Kruger, Avery A1 - Inderbitzen, Katherine A1 - Le, Jennifer A1 - others JF - Oceanography PB - OCEANOGRAPHY SOC PO BOX 1931, ROCKVILLE, MD USA VL - 29 IS - 1 ER - TY - JOUR T1 - Heterotrophic Proteobacteria in the vicinity of diffuse hydrothermal venting JF - Environmental microbiology Y1 - 2016 A1 - Meier, Dimitri V A1 - Bach, Wolfgang A1 - Girguis, Peter R. A1 - Gruber-Vodicka, Harald R A1 - Reeves, Eoghan P A1 - Richter, Michael A1 - Vidoudez, Charles A1 - Amann, Rudolf A1 - Meyerdierks, Anke VL - 18 IS - 12 ER - TY - JOUR T1 - Independent benthic microbial fuel cells powering sensors and acoustic communications with the MARS underwater observatory JF - Journal of Atmospheric and Oceanic Technology Y1 - 2016 A1 - Schrader, Paul S A1 - Reimers, Clare E A1 - Peter Girguis A1 - Delaney, Jennifer A1 - Cody Doolan A1 - Michael Wolf A1 - Dale Green VL - 33 IS - 3 ER - TY - JOUR T1 - Intracellular carbon isotope distributions of continuous-culture Allochromatium vinosum grown on acetate vs. CO 2. JF - American Geophysical Union Y1 - 2016 A1 - Tang, T A1 - Mohr, W A1 - Sattin, S A1 - Rogers, D A1 - Girguis, P. R. A1 - Pearson, A VL - 2016 ER - TY - JOUR T1 - Investigating the Differences in the Total and Active Microbial Community of Mid-Atlantic Ridge Sediments JF - American Geophysical Union Y1 - 2016 A1 - Sobol, Morgan S A1 - Zinke, Laura A A1 - Orcutt, Beth A1 - Mills, Heath Jordan A1 - Edwards, Katrina J A1 - Girguis, Peter R. A1 - Reese, BK VL - 2016 ER - TY - CONF T1 - Metagenomic Assessment of a Dynamic Microbial Population from Subseafloor Aquifer Fluids in the Cold, Oxygenated Crust T2 - AGU Fall Meeting Abstracts Y1 - 2016 A1 - Tully, Benjamin J A1 - Heidelberg, John F A1 - Kraft, Beate A1 - Girguis, Peter R. A1 - Huber, Julie A JF - AGU Fall Meeting Abstracts VL - 2016 ER - TY - JOUR T1 - Metatranscriptional response of chemoautotrophic Ifremeria nautilei endosymbionts to differing sulfur regimes JF - Frontiers in microbiology Y1 - 2016 A1 - Seston, Sherry L A1 - Beinart, Roxanne A A1 - Sarode, Neha A1 - Shockey, Abigail C A1 - Ranjan, Piyush A1 - Ganesh, Sangita A1 - Girguis, Peter R. A1 - Stewart, Frank J PB - Frontiers VL - 7 ER - TY - JOUR T1 - Microbial ecology: Here, there and everywhere JF - Nature Microbiology Y1 - 2016 A1 - Peter Girguis PB - Nature Publishing Group VL - 1 IS - 8 ER - TY - JOUR T1 - Nanoporous microscale microbial incubators JF - Lab on a Chip Y1 - 2016 A1 - Ge, Zhifei A1 - Girguis, Peter R. A1 - Buie, Cullen R PB - Royal Society of Chemistry VL - 16 IS - 3 ER - TY - JOUR T1 - NC10 bacteria in marine oxygen minimum zones JF - The ISME journal Y1 - 2016 A1 - Padilla,Cory C A1 - Bristow,Laura A A1 - Sarode, Neha A1 - Garcia-Robledo,Emilio A1 - Gómez Ram{\'ırez, Eddy A1 - Benson,Catherine R A1 - Bourbonnais,Annie A1 - Altabet,Mark A A1 - Girguis, Peter R. A1 - Thamdrup,Bo A1 - others PB - Nature Publishing Group VL - 10 IS - 8 ER - TY - JOUR T1 - Patterns of sulfur isotope fractionation during microbial sulfate reduction JF - Geobiology Y1 - 2016 A1 - Bradley, A. S. A1 - Leavitt, WD A1 - Schmidt, M. A1 - Knoll, Andrew Herbert A1 - Girguis, Peter R. A1 - Johnston, David T VL - 14 IS - 1 ER - TY - JOUR T1 - ROV Hercules Investigates Brine Lakes on the Bottom of the Ocean JF - Oceanography Y1 - 2016 A1 - Cordes, E A1 - Michel, A A1 - Petersen, J A1 - Wankel, S A1 - Ansorge, Rebecca A1 - Girguis, P. A1 - Leisch, Nikolaus A1 - Smart, Clara A1 - Roman, Chris A1 - Wetzel, Silke A1 - others VL - 29 IS - 1 Suppl. S ER - TY - CONF T1 - Stability of Hydrothermal Vent Communities on the Eastern Lau Spreading Center T2 - AGU Fall Meeting Abstracts Y1 - 2016 A1 - Fisher, Charles R A1 - Du Preez, Cherisse A1 - Ferrini, Vicki Lynn A1 - Beinart, Roxanne A1 - Seewald, Jeffrey A1 - Hoer, Daniel A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2016 ER - TY - JOUR T1 - (Tele) presenting Secrets from the Deep Southern California Margin JF - American Geophysical Union Y1 - 2016 A1 - Levin, Lisa A A1 - Girguis, Peter R. A1 - Brennan, Michael A1 - German, Christopher R A1 - Raineault, Nicole A1 - Le, Jennifer Tran A1 - Grupe, Benjamin A1 - Gallo, Natalya A1 - Inderbitzen, Katherine E A1 - Tuzun, Suna A1 - others VL - 2016 ER - TY - JOUR T1 - What do we really know about the role of microorganisms in iron sulfide mineral formation? JF - Frontiers in Earth Science Y1 - 2016 A1 - Picard, Aude A1 - Gartman, Amy A1 - Girguis, Peter R. PB - Frontiers VL - 4 ER - TY - JOUR T1 - Arsenic concentrations and species in three hydrothermal vent worms, Ridgeia piscesae, Paralvinella sulficola and Paralvinella palmiformis JF - Deep Sea Research Part I: Oceanographic Research Papers Y1 - 2016 A1 - W.A. Maher A1 - E. Duncan A1 - G. Dilly A1 - S. Foster A1 - F. Krikowa A1 - E. Lombi A1 - K. Scheckel A1 - Girguis, P. AB - Abstract Hydrothermal vents are surficial expressions of subsurface geological and hydrological processes. Fluids emitting from active vents are chemically distinct from bottom seawater, and are enriched in dissolved metals and metalloids, including arsenic. Vent organisms accumulate arsenic but the arsenic speciation in these non-photosynthetic organisms is largely unknown. Here, arsenic concentrations and chemical species were measured in three deep sea hydrothermal vent worms (Ridgeia piscesae, Paralvinella sulfincola and Paralvinella palmiformis ) from the Juan de Fuca Ridge in the Northwest pacific. R. piscesae has similar arsenic concentrations (3.8–35 µg g−1) to shallow water polychaetes while P. sulfincola and P. palmiformis have significantly higher arsenic concentrations (420–1417 and 125–321 µg g−1 respectively). R. piscesae contains appreciable quantities of inorganic arsenic (36±14%), monomethyl arsenic (2±2%), dimethyl arsenic (34±21%), an unknown methyl arsenical (7±16%), OSO3-arsenosugar (5±9%), \TETRA\ (4±5%), ThioPO4/ThioDMAE (1±2%) and an unknown thio-arsenical (12±14%). These results suggests that host and symbionts are either involved in the methylation of arsenic, or are bathed in fluids enriched in methylated arsenic as a result of free-living microbial activity. The host carrying out methylation, however, cannot be ruled out. In contrast, 96–97% of the arsenic in P. sulfincola and P. palmiformis is inorganic arsenic, likely the result of arsenic precipitation within and upon the mucus they ingest while feeding. While all worms have oxo- and thio arsenosugars (2–30%), Paralvinella also have small amounts of arsenobetaine (<0.001–0.21%). The presence of arsenosugars, arsenobetaine and other minor arsenic species in the absence of photosynthesising algae/bacteria indicates that they may be formed by vent animals in the absence of sunlight, but at this time their formation cannot be explained. VL - 116 UR - http://www.sciencedirect.com/science/article/pii/S0967063716301194 ER - TY - JOUR T1 - Microbial ecology: Here, there and everywhere JF - Nature Microbiology Y1 - 2016 A1 - Peter Girguis PB - Macmillan Publishers Limited VL - 1 UR - http://dx.doi.org/10.1038/nmicrobiol.2016.123 ER - TY - JOUR T1 - Heterotrophic Proteobacteria in the vicinity of diffuse hydrothermal venting JF - Environmental Microbiology Y1 - 2016 A1 - Meier, Dimitri V. A1 - Bach, Wolfgang A1 - Peter R. Girguis A1 - Gruber-Vodicka, Harald R. A1 - Reeves, Eoghan P. A1 - Richter, Michael A1 - Vidoudez, Charles A1 - Amann, Rudolf A1 - Meyerdierks, Anke AB - Deep-sea hydrothermal vents are highly dynamic habitats characterized by steep temperature and chemical gradients. The oxidation of reduced compounds dissolved in the venting fluids fuels primary production providing the basis for extensive life. Until recently studies of microbial vent communities have focused primarily on chemolithoautotrophic organisms. In our study, we targeted the change of microbial community compositions along mixing gradients, focusing on distribution and capabilities of heterotrophic microorganisms. Samples were retrieved from different venting areas within the Menez Gwen hydrothermal field, taken along mixing gradients, including diffuse fluid discharge points, their immediate surroundings and the buoyant parts of hydrothermal plumes. High throughput 16S rRNA gene amplicon sequencing, fluorescence in situ hybridization, and targeted metagenome analysis were combined with geochemical analyses. Close to diffuse venting orifices dominated by chemolithoautotrophic Epsilonproteobacteria, in areas where environmental conditions still supported chemolithoautotrophic processes, we detected microbial communities enriched for versatile heterotrophic Alpha- and Gammaproteobacteria. The potential for alkane degradation could be shown for several genera and yet uncultured clades. We propose that hotspots of chemolithoautotrophic life support a ‘belt’ of heterotrophic bacteria significantly different from the dominating oligotrophic microbiota of the deep sea. UR - http://dx.doi.org/10.1111/1462-2920.13304 ER - TY - JOUR T1 - Independent Benthic Microbial Fuel Cells Powering Sensors and Acoustic Communications with the MARS Underwater Observatory JF - Journal of Atmospheric and Oceanic Technology Y1 - 2016 A1 - Paul S. Schrader A1 - Clare E. Reimers A1 - Peter Girguis A1 - Delaney, Jennifer A1 - Cody Doolan A1 - Michael Wolf A1 - Dale Green AB - AbstractMost oceanographic instruments on the seafloor have no connections with the surface and therefore have to run on batteries and store data until recovery. To demonstrate a developing technology, sensors and acoustic modems were powered with energy harvested from the seafloor, and data were relayed acoustically in near–real time to the Monterey Accelerated Research System (MARS) observatory in Monterey Bay, California, and to surface research vessels. MARS is a cabled observatory in deep water ( 890 m) at the edge of Monterey Canyon. An acoustic modem was attached to the MARS node and configured to send out commands to, and relay data received from, remote modems. Two benthic microbial fuel cells (BMFCs) positioned approximately 0.5 km away from MARS supplied power to the remote modems and sensors. At their peak performance, these BMFCs produced continuous power densities of  35 mW m−2 (footprint area). The modems utilized in this study contained an integrated power management platform (PMP) designed to manage and store the electrical energy generated by each BMFC and to record BMFC performance parameters and sensor data on an hourly basis. Temperature and either oxygen or conductivity sensors were chosen because of their common use and environmental relevance. Acoustically transmitted data records show that the BMFCs renewed energy stores and that the oceanographic sensors measured dissolved oxygen, temperature, and conductivity reliably throughout the operational life of each BMFC system ( 6 months). These systems remained in place for more than 12 months. VL - 33 UR - http://dx.doi.org/10.1175/JTECH-D-15-0102.1 IS - 3 ER - TY - JOUR T1 - NC10 bacteria in marine oxygen minimum zones JF - ISME J Y1 - 2016 A1 - Padilla,Cory C A1 - Bristow,Laura A A1 - Sarode, Neha A1 - Garcia-Robledo,Emilio A1 - Gomez Ramirez,Eddy A1 - Benson,Catherine R A1 - Bourbonnais,Annie A1 - Altabet,Mark A A1 - Girguis, Peter R. A1 - Thamdrup,Bo A1 - Stewart, Frank J AB - Bacteria of the NC10 phylum link anaerobic methane oxidation to nitrite denitrification through a unique O2-producing intra-aerobic methanotrophy pathway. A niche for NC10 in the pelagic ocean has not been confirmed. We show that NC10 bacteria are present and transcriptionally active in oceanic oxygen minimum zones (OMZs) off northern Mexico and Costa Rica. NC10 16S rRNA genes were detected at all sites, peaking in abundance in the anoxic zone with elevated nitrite and methane concentrations. Phylogenetic analysis of particulate methane monooxygenase genes further confirmed the presence of NC10. rRNA and mRNA transcripts assignable to NC10 peaked within the OMZ and included genes of the putative nitrite-dependent intra-aerobic pathway, with high representation of transcripts containing the unique motif structure of the nitric oxide (NO) reductase of NC10 bacteria, hypothesized to participate in O2-producing NO dismutation. These findings confirm pelagic OMZs as a niche for NC10, suggesting a role for this group in OMZ nitrogen, methane and oxygen cycling. PB - International Society for Microbial Ecology VL - 10 SN - 1751-7362 UR - http://dx.doi.org/10.1038/ismej.2015.262 IS - 8 N1 - Supplementary information available for this article at http://www.nature.com/ismej/journal/v10/n8/suppinfo/ismej2015262s1.html ER - TY - JOUR T1 - Metatranscriptional Response of Chemoautotrophic Ifremeria nautilei Endosymbionts to Differing Sulfur Regimes JF - Frontiers in Microbiology Y1 - 2016 A1 - Seston, Sherry L A1 - Beinart, Roxanne A A1 - Sarode, Neha A1 - Shockey, Abigail C A1 - Ranjan, Piyush A1 - Ganesh, Sangita A1 - Girguis, Peter R. A1 - Stewart, Frank J AB - Endosymbioses between animals and chemoautotrophic bacteria are ubiquitous at hydrothermal vents. These environments are distinguished by high physico-chemical variability, yet we know little about how these symbioses respond to environmental fluctuations. We therefore examined how the γ-proteobacterial symbionts of the vent snail Ifremeria nautilei respond to changes in sulfur geochemistry. Via shipboard high-pressure incubations, we subjected snails to 105 μM hydrogen sulfide (LS), 350 μM hydrogen sulfide (HS), 300 μM thiosulfate (TS) and seawater without any added inorganic electron donor (ND). While transcript levels of sulfur oxidation genes were largely consistent across treatments, HS and TS treatments stimulated genes for denitrification, nitrogen assimilation, and CO(2) fixation, coincident with previously reported enhanced rates of inorganic carbon incorporation and sulfur oxidation in these treatments. Transcripts for genes mediating oxidative damage were enriched in the ND and LS treatments, potentially due to a reduction in O(2) scavenging when electron donors were scarce. Oxidative TCA cycle gene transcripts were also more abundant in ND and LS treatments, suggesting that I. nautilei symbionts may be mixotrophic when inorganic electron donors are limiting. These data reveal the extent to which I. nautilei symbionts respond to changes in sulfur concentration and species, and, interpreted alongside coupled biochemical metabolic rates, identify gene targets whose expression patterns may be predictive of holobiont physiology in environmental samples. PB - Frontiers Media S.A. VL - 7 SN - 1664-302X UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4949241/ U1 - 27486438[pmid] JO - Front Microbiol ER - TY - JOUR T1 - What Do We Really Know about the Role of Microorganisms in Iron Sulfide Mineral Formation? JF - Frontiers in Earth Science Y1 - 2016 A1 - Picard, Aude A1 - Gartman, Amy A1 - Peter R. Girguis AB - Iron sulfide mineralization in low-temperature systems is a result of biotic and abiotic processes, though the delineation between these two modes of formation is not always straightforward. Here we review the role of microorganisms in the precipitation of extracellular iron sulfide minerals. We summarize the evidence that links sulfur-metabolizing microorganisms and sulfide minerals in nature and we present a critical overview of laboratory-based studies of the nucleation and growth of iron sulfide minerals in microbial cultures. We discuss whether biologically derived minerals are distinguishable from abiotic minerals, possessing attributes that are uniquely diagnostic of biomineralization. These inquiries have revealed the need for additional thorough, mechanistic and high-resolution studies to understand microbially mediated formation of a variety of sulfide minerals across a range of natural environments. VL - 4 UR - http://journal.frontiersin.org/article/10.3389/feart.2016.00068 ER - TY - JOUR T1 - A distinct and active bacterial community in cold oxygenated fluids circulating beneath the western flank of the Mid-Atlantic ridge JF - Scientific Reports Y1 - 2016 A1 - Meyer, Julie L A1 - Jaekel, Ulrike A1 - Tully, Benjamin J A1 - Glazer, Brian T A1 - Wheat, C Geoffrey A1 - Lin, Huei-Ting A1 - Hsieh, Chih-Chiang A1 - Cowen, James P A1 - Hulme, Samuel M A1 - Girguis, Peter R. A1 - Huber, Julie A AB - The rock-hosted, oceanic crustal aquifer is one of the largest ecosystems on Earth, yet little is known about its indigenous microorganisms. Here we provide the first phylogenetic and functional description of an active microbial community residing in the cold oxic crustal aquifer. Using subseafloor observatories, we recovered crustal fluids and found that the geochemical composition is similar to bottom seawater, as are cell abundances. However, based on relative abundances and functional potential of key bacterial groups, the crustal fluid microbial community is heterogeneous and markedly distinct from seawater. Potential rates of autotrophy and heterotrophy in the crust exceeded those of seawater, especially at elevated temperatures (25 °C) and deeper in the crust. Together, these results reveal an active, distinct, and diverse bacterial community engaged in both heterotrophy and autotrophy in the oxygenated crustal aquifer, providing key insight into the role of microbial communities in the ubiquitous cold dark subseafloor biosphere. PB - Nature Publishing Group VL - 6 SN - 2045-2322 UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776111/ U1 - 26935537[pmid] JO - Sci Rep ER - TY - JOUR T1 - Nanoporous microscale microbial incubators JF - Lab Chip Y1 - 2016 A1 - Ge, Zhifei A1 - Peter R. Girguis A1 - Buie, Cullen R. AB - Reconstruction of phylogenetic trees based on 16S rRNA gene sequencing reveals abundant microbial diversity that has not been cultured in the laboratory. Many attribute this so-called 'great plate count anomaly' to traditional microbial cultivation techniques, which largely facilitate the growth of a single species. Yet, it is widely recognized that bacteria in nature exist in complex communities. One technique to increase the pool of cultivated bacterial species is to co-culture multiple species in a simulated natural environment. Here, we present nanoporous microscale microbial incubators (NMMI) that enable high-throughput screening and real-time observation of multi-species co-culture. The key innovation in NMMI is that they facilitate inter-species communication while maintaining physical isolation between species, which is ideal for genomic analysis. Co-culture of a quorum sensing pair demonstrates that the NMMI can be used to culture multiple species in chemical communication while monitoring the growth dynamics of individual species. PB - The Royal Society of Chemistry VL - 16 UR - http://dx.doi.org/10.1039/C5LC00978B ER - TY - JOUR T1 - Patterns of sulfur isotope fractionation during microbial sulfate reduction JF - Geobiology Y1 - 2016 A1 - Bradley, A. S. A1 - Leavitt, W. D. A1 - Schmidt, M. A1 - Knoll, A. H. A1 - Girguis, P. R. A1 - Johnston, D. T. AB - Studies of microbial sulfate reduction have suggested that the magnitude of sulfur isotope fractionation varies with sulfate concentration. Small apparent sulfur isotope fractionations preserved in Archean rocks have been interpreted as suggesting Archean sulfate concentrations of <200 μm, while larger fractionations thereafter have been interpreted to require higher concentrations. In this work, we demonstrate that fractionation imposed by sulfate reduction can be a function of concentration over a millimolar range, but that nature of this relationship depends on the organism studied. Two sulfate-reducing bacteria grown in continuous culture with sulfate concentrations ranging from 0.1 to 6 mm showed markedly different relationships between sulfate concentration and isotope fractionation. Desulfovibrio vulgaris str. Hildenborough showed a large and relatively constant isotope fractionation (34εSO4-H2S ≅ 25‰), while fractionation by Desulfovibrio alaskensis G20 strongly correlated with sulfate concentration over the same range. Both data sets can be modeled as Michaelis–Menten (MM)-type relationships but with very different MM constants, suggesting that the fractionations imposed by these organisms are highly dependent on strain-specific factors. These data reveal complexity in the sulfate concentration–fractionation relationship. Fractionation during MSR relates to sulfate concentration but also to strain-specific physiological parameters such as the affinity for sulfate and electron donors. Previous studies have suggested that the sulfate concentration–fractionation relationship is best described with a MM fit. We present a simple model in which the MM fit with sulfate concentration and hyperbolic fit with growth rate emerge from simple physiological assumptions. As both environmental and biological factors influence the fractionation recorded in geological samples, understanding their relationship is critical to interpreting the sulfur isotope record. As the uptake machinery for both sulfate and electrons has been subject to selective pressure over Earth history, its evolution may complicate efforts to uniquely reconstruct ambient sulfate concentrations from a single sulfur isotopic composition. VL - 14 UR - http://dx.doi.org/10.1111/gbi.12149 IS - 1 ER - TY - CONF T1 - Advancing a deep sea near-infrared laser spectrometer for dual isotope measurements T2 - CLEO: Applications and Technology Y1 - 2015 A1 - Michel, Anna PM A1 - Wankel, Scott D A1 - Kapit, Jason A1 - Girguis, Peter R. A1 - Manish Gupta JF - CLEO: Applications and Technology PB - Optical Society of America ER - TY - JOUR T1 - Baleen whales host a unique gut microbiome with similarities to both carnivores and herbivores JF - Nature communications Y1 - 2015 A1 - Sanders, Jon G A1 - Beichman, Annabel C A1 - Joe Roman A1 - Scott, Jarrod J A1 - David Emerson A1 - McCarthy, James J A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 6 IS - 1 ER - TY - JOUR T1 - Biological capacitance studies of anodes in microbial fuel cells using electrochemical impedance spectroscopy JF - Bioprocess and biosystems engineering Y1 - 2015 A1 - Lu, Zhihao A1 - Peter Girguis A1 - Liang, Peng A1 - Shi, Haifeng A1 - Huang, Guangtuan A1 - Cai, Lankun A1 - Zhang, Lehua PB - Springer Berlin Heidelberg VL - 38 IS - 7 ER - TY - JOUR T1 - Carbon fixation by basalt-hosted microbial communities JF - Frontiers in Microbiology Y1 - 2015 A1 - Orcutt, Beth N A1 - Sylvan, Jason B A1 - Rogers, Daniel A1 - Delaney, Jennifer A1 - Lee, Raymond W A1 - Girguis, Peter R. PB - Frontiers VL - 6 ER - TY - JOUR T1 - Collaborative Research: Ecosystem dynamics of Western Pacific hydrothermal vent communities associated with polymetallic sulfide deposits Y1 - 2015 A1 - Peter Girguis A1 - others ER - TY - JOUR T1 - COLLABORATIVE RESEARCH: The role of iron-oxidizing bacteria in the sedimentary iron cycle: ecological, physiological and biogeochemical implications. Y1 - 2015 A1 - Peter Girguis A1 - others ER - TY - JOUR T1 - The ecological physiology of Earth's second oxygen revolution JF - Annual Review of Ecology, Evolution, and Systematics Y1 - 2015 A1 - Sperling, Erik A A1 - Knoll, Andrew H A1 - Girguis, Peter R. PB - Annual Reviews VL - 46 ER - TY - JOUR T1 - Enhancing the response of microbial fuel cell based toxicity sensors to Cu (II) with the applying of flow-through electrodes and controlled anode potentials JF - Bioresource technology Y1 - 2015 A1 - Jiang, Yong A1 - Liang, Peng A1 - Zhang, Changyong A1 - Bian, Yanhong A1 - Yang, Xufei A1 - Huang, Xia A1 - Girguis, Peter R. PB - Elsevier VL - 190 ER - TY - JOUR T1 - Graphite Anodes Activated by Melamine, Carbamide, ZnCl2 and H3PO4 in Microbial Fuel Cells JF - Int. J. Electrochem. Sci Y1 - 2015 A1 - Qiao, Junjing A1 - Peter Girguis A1 - Li, Dongmei A1 - Ma, Jingxing A1 - Cai, Lankun A1 - Zhang, Lehua VL - 10 ER - TY - JOUR T1 - Key factors influencing rates of heterotrophic sulfate reduction in active seafloor hydrothermal massive sulfide deposits JF - Frontiers in microbiology Y1 - 2015 A1 - Frank, Kiana L A1 - Rogers, Karyn L A1 - Rogers, Daniel R A1 - Johnston, David T A1 - Girguis, Peter R. PB - Frontiers VL - 6 ER - TY - CONF T1 - Key Factors Influencing Rates of Heterotrophic Sulfate Reduction in Hydrothermal Massive Sulfide Deposits T2 - AGU Fall Meeting Abstracts Y1 - 2015 A1 - Frank, Kiana L A1 - Rogers, Karyn L A1 - Rogers, Daniel A1 - Johnston, David T A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2015 ER - TY - CONF T1 - Microbe-mediated transformations of marine dissolved organic matter during 2,100 years of natural incubation in the cold, oxic crust of the Mid-Atlantic Ridge. T2 - AGU Fall Meeting Abstracts Y1 - 2015 A1 - Shah Walter, SR A1 - Jaekel, Ulrike A1 - Huber, Julie A A1 - Dittmar, Thorsten A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2015 ER - TY - JOUR T1 - Ubiquitous presence and novel diversity of anaerobic alkane degraders in cold marine sediments JF - Frontiers in Microbiology Y1 - 2015 A1 - Gittel, Antje A1 - Donhauser, Johanna A1 - Røy, Hans A1 - Girguis, Peter R. A1 - Jørgensen, Bo B A1 - Kjeldsen, Kasper U PB - Frontiers VL - 6 ER - TY - JOUR T1 - The uptake and excretion of partially oxidized sulfur expands the repertoire of energy resources metabolized by hydrothermal vent symbioses JF - Proceedings of the Royal Society B: Biological Sciences Y1 - 2015 A1 - Beinart, R. A. A1 - Gartman, A. A1 - Sanders, J. G. A1 - Luther, G. W. A1 - Girguis, P. R. PB - The Royal Society VL - 282 IS - 1806 ER - TY - CONF T1 - When Worlds Collide: Microbial Ecophysiology at the Aerobic/Anaerobic Interface T2 - AGU Fall Meeting Abstracts Y1 - 2015 A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2015 ER - TY - JOUR T1 - Baleen whales host a unique gut microbiome with similarities to both carnivores and herbivores JF - Nature Communications Y1 - 2015 A1 - Jon G. Sanders A1 - Annabel C. Beichman A1 - Joe Roman A1 - Jarrod J. Scott A1 - David Emerson A1 - James J. McCarthy A1 - Peter R. Girguis PB - The Author(s) VL - 6 UR - http://dx.doi.org/10.1038/ncomms9285 ER - TY - JOUR T1 - Key Factors Influencing Rates of Heterotrophic Sulfate Reduction in Active Seafloor Hydrothermal Massive Sulfide Deposits JF - Frontiers in Microbiology Y1 - 2015 A1 - Frank, Kiana L. A1 - Rogers, Karyn L. A1 - Rogers, Daniel R. A1 - Johnston, David T. A1 - Peter R. Girguis AB - Hydrothermal vents are thermally and geochemically dynamic habitats, and the organisms therein are subject to steep gradients in temperature and chemistry. To date, the influence of these environmental dynamics on microbial sulfate reduction has not been well constrained. Here, via multivariate experiments, we evaluate the effects of key environmental variables (temperature, pH, H2S, SO42-, DOC) on sulfate reduction rates and metabolic energy yields in material recovered from a hydrothermal flange from the Grotto edifice in the Main Endeavor Field, Juan de Fuca Ridge. Sulfate reduction was measured in batch reactions across a range of physico-chemical conditions. Temperature and pH were the strongest stimuli, and maximum sulfate reduction rates were observed at 50 °C and pH 6, suggesting that the in situ community of sulfate-reducing organisms in Grotto flanges may be most active in a slightly acidic and moderate thermal/chemical regime. At pH 4, sulfate reduction rates increased with sulfide concentrations most likely due to the mitigation of metal toxicity. While substrate concentrations also influenced sulfate reduction rates, energy-rich conditions muted the effect of metabolic energetics on sulfate reduction rates. We posit that variability in sulfate reduction rates reflect the response of the active microbial consortia to environmental constraints on in situ microbial physiology, toxicity, and the type and extent of energy limitation. These experiments help to constrain models of the spatial contribution of heterotrophic sulfate reduction within the complex gradients inherent to seafloor hydrothermal deposits. VL - 6 UR - http://journal.frontiersin.org/article/10.3389/fmicb.2015.01449 ER - TY - JOUR T1 - Ubiquitous Presence and Novel Diversity of Anaerobic Alkane Degraders in Cold Marine Sediments JF - Frontiers in Microbiology Y1 - 2015 A1 - Gittel, Antje A1 - Donhauser, Johanna A1 - Røy, Hans A1 - Peter R. Girguis A1 - Jørgensen, Bo B. A1 - Kjeldsen, Kasper U. AB - Alkanes are major constituents of crude oil and are released to the marine environment by natural seepage and from anthropogenic sources. Due to their chemical inertness, their removal from anoxic marine sediments is primarily controlled by the activity of anaerobic alkane-degrading microorganisms. To conduct a comprehensive cultivation-independent survey of the diversity and distribution of anaerobic alkane degraders, we designed novel PCR primers that cover all known diversity of the 1-methylalkyl succinate synthase gene (masD/assA), which catalyzes the initial activation of alkanes. We studied masD/assA gene diversity in pristine and seepage-impacted Danish coastal sediments, as well as in sediments and alkane-degrading enrichment cultures from the Middle Valley (MV) hydrothermal vent system in the Pacific Northwest. MasD/assA genes were ubiquitously present, and the primers captured the diversity of both known and previously undiscovered masD/assA gene diversity. Seepage sediments were dominated by a single masD/assA gene cluster, which is presumably indicative of a substrate-adapted community, while pristine sediments harbored a diverse range of masD/assA phylotypes including those present in seepage sediments. This rare biosphere of anaerobic alkane degraders will likely increase in abundance in the event of seepage or accidental oil spillage. Nanomolar concentrations of short-chain alkanes (SCA) were detected in pristine and seepage sediments. Interestingly, anaerobic alkane degraders closely related to strain BuS5, the only SCA degrader in pure culture, were found in mesophilic MV enrichments, but not in cold sediments from Danish waters. We propose that the new masD/assA gene lineages in these sediments represent novel phylotypes that are either fueled by naturally occurring low levels of SCA or that metabolize medium- to long-chain alkanes. Our study highlights that masD/assA genes are a relevant diagnostic marker to identify seepage and microseepage, e.g. during prospecting for oil and gas, and may act as an indicator of anthropogenic oil spills in marine sediments. VL - 6 UR - http://journal.frontiersin.org/article/10.3389/fmicb.2015.01414 ER - TY - JOUR T1 - Carbon fixation by basalt-hosted microbial communities JF - Frontiers in Microbiology Y1 - 2015 A1 - Orcutt, Beth N A1 - Sylvan, Jason B A1 - Rogers, Daniel R A1 - Delaney, Jennifer A1 - Lee, Raymond W A1 - Girguis, Peter R. AB - Oceanic crust is a massive potential habitat for microbial life on Earth, yet our understanding of this ecosystem is limited due to difficulty in access. In particular, measurements of rates of microbial activity are sparse. We used stable carbon isotope incubations of crustal samples, coupled with functional gene analyses, to examine the potential for carbon fixation on oceanic crust. Both seafloor-exposed and subseafloor basalts were recovered from different mid-ocean ridge and hot spot environments (i.e., the Juan de Fuca Ridge, the Mid-Atlantic Ridge, and the Loihi Seamount) and incubated with (13)C-labeled bicarbonate. Seafloor-exposed basalts revealed incorporation of (13)C-label into organic matter over time, though the degree of incorporation was heterogeneous. The incorporation of (13)C into biomass was inconclusive in subseafloor basalts. Translating these measurements into potential rates of carbon fixation indicated that 0.1–10 nmol C g(-1)(rock) d(-1) could be fixed by seafloor-exposed rocks. When scaled to the global production of oceanic crust, this suggests carbon fixation rates of 10(9)–10(12) g C year(-1), which matches earlier predictions based on thermodynamic calculations. Functional gene analyses indicate that the Calvin cycle is likely the dominant biochemical mechanism for carbon fixation in basalt-hosted biofilms, although the reductive acetyl-CoA pathway and reverse TCA cycle likely play some role in net carbon fixation. These results provide empirical evidence for autotrophy in oceanic crust, suggesting that basalt-hosted autotrophy could be a significant contributor of organic matter in this remote and vast environment. PB - Frontiers Media S.A. VL - 6 SN - 1664-302X UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561358/ U1 - 26441854[pmid] JO - Front Microbiol ER - TY - JOUR T1 - Biological capacitance studies of anodes in microbial fuel cells using electrochemical impedance spectroscopy. JF - Bioprocess and Biosystems Engineering Y1 - 2015 A1 - Lu, Z. A1 - Girguis, P. A1 - Liang, P. A1 - Shi, H. A1 - Huang, G. A1 - Cai, L. A1 - Zhang, L. VL - 38 ER - TY - JOUR T1 - Enhancing the response of microbial fuel cell based toxicity sensors to Cu (II) with the applying of flow-through electrodes and controlled anode potentials. JF - Bioresource Technology Y1 - 2015 A1 - Jiang, Y. A1 - Liang, P. A1 - Zhang, C. A1 - Bian, Y. A1 - Yang, X. A1 - Huang, X. A1 - Girguis, PR. VL - 190 ER - TY - JOUR T1 - Patterns of microbial sulfur isotope fractionation at low sulfate concentrations. JF - Geobiology Y1 - 2015 A1 - Bradley, AS. A1 - Leavitt, WD. A1 - Schmidt, M. A1 - Knoll, AH. A1 - Girguis, PR. A1 - Johnston, DT. ER - TY - JOUR T1 - The uptake and excretion of partially oxidized sulfur expands the repertoire of energy resources metabolized by hydrothermal vent symbioses. JF - Proceedings of the Royal Society Biological Sciences Y1 - 2015 A1 - Beinart, RA. A1 - Gartman, A. A1 - Sanders, JG. A1 - Luther, GW. A1 - Girguis, PR. VL - 282 ER - TY - CONF T1 - Access to the Sea: A Roadmap for Expedition Planning T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - DeSilva, Annette M A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - CONF T1 - Carbon fixation in oceanic crust: Does it happen, and is it important? T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Orcutt, Beth A1 - Sylvan, Jason B A1 - Rogers, Daniel A1 - Lee, Ray A1 - Girguis, Peter R. A1 - Carr, Stephanie A A1 - Jungbluth, Sean A1 - Rappe, Michael S JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - JOUR T1 - Characterizing the plasticity of nitrogen metabolism by the host and symbionts of the hydrothermal vent chemoautotrophic symbioses R idgeia piscesae JF - Molecular ecology Y1 - 2014 A1 - Liao, Li A1 - Wankel, Scott D A1 - Wu, Min A1 - Cavanaugh, Colleen M A1 - Girguis, Peter R. VL - 23 IS - 6 ER - TY - ABST T1 - Citation for Scientific Excellence: Victoria J. Bertics Y1 - 2014 A1 - Girguis, Peter R. ER - TY - CONF T1 - Constraining geochemistry and biological primary productivity in hydrothermal systems via in situ mass spectrometric geochemical mapping T2 - EGU General Assembly Conference Abstracts Y1 - 2014 A1 - Vidoudez, Charles A1 - Marcon, Yann A1 - Bach, Wolfgang A1 - Lebris, Nadine A1 - Dubilier, Nicole A1 - Peter Girguis JF - EGU General Assembly Conference Abstracts ER - TY - JOUR T1 - Electron uptake by iron-oxidizing phototrophic bacteria JF - Nature communications Y1 - 2014 A1 - Bose, Arpita A1 - Gardel, Emily Jeanette A1 - Vidoudez, Charles A1 - Parra, E. A. A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 5 IS - 1 ER - TY - CONF T1 - Finding the best windows: An apparent environmental threshold determines which diffuse flows are dominated by subsurface microbes T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Olins, Heather C A1 - Rogers, Daniel A1 - Scholin, Chris A A1 - Preston, Chris J A1 - Vidoudez, Charles A1 - Ussler, William A1 - Pargett, Doug A1 - Jensen, Scott A1 - Roman, Brent A1 - Birch, James M A1 - others JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - CONF T1 - From Mantle to Microbe to Mollusc: How Animal-Microbial Symbioses Influence Carbon and Sulfur Cycling in Hydrothermal Vent Flows. T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Girguis, Peter R. A1 - Beinart, Roxanne JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - JOUR T1 - Intracellular O ceanospirillales inhabit the gills of the hydrothermal vent snail A lviniconcha with chemosynthetic, $\gamma$-P roteobacterial symbionts JF - Environmental Microbiology Reports Y1 - 2014 A1 - Beinart, R. A. A1 - Nyholm, S. V. A1 - Dubilier, Nicole A1 - Girguis, Peter R. VL - 6 IS - 6 ER - TY - CONF T1 - Islands in the Sea: the Patchy Distribution and Physiological Poise of Vent Microbes and the Implications for Carbon Cycling T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - CONF T1 - Microbial Turnover of Fixed Nitrogen Compounds in Oceanic Crustal Fluids T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Kraft, Beate A1 - Wankel, Scott D A1 - Glazer, Brian T A1 - Huber, Julie A A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - CONF T1 - Oxygen, ecology, and the Cambrian radiation of animals T2 - INTEGRATIVE AND COMPARATIVE BIOLOGY Y1 - 2014 A1 - Sperling, E. A. A1 - Frieder, C. A. A1 - Raman, A A1 - Girguis, P. R. A1 - Levin, L. A. A1 - Knoll, A. H. JF - INTEGRATIVE AND COMPARATIVE BIOLOGY PB - OXFORD UNIV PRESS INC JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA VL - 54 ER - TY - CONF T1 - Perspectives on Applying Metabolomics to Understand Carbon Cycling and Process Rates in Deep-Sea Microorganisms T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Vidoudez, Charles A1 - Saghatelian, Alan A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - JOUR T1 - Project Summary: Investigations of Chemosynthetic Communities on the Lower Continental Slope of the Gulf of Mexico Y1 - 2014 A1 - Brooks, JM A1 - Fisher, C A1 - Roberts, H A1 - Bernard, B A1 - McDonald, I A1 - Carney, R A1 - Joye, S A1 - Cordes, E A1 - Wolff, G A1 - Bright, M. A1 - others PB - United States. Bureau of Ocean Energy Management. ER - TY - CONF T1 - Sulfate Reduction and Sulfide Biomineralization by Deep-Sea Hydrothermal Vent Microorganisms T2 - AGU Fall Meeting Abstracts Y1 - 2014 A1 - Picard, Aude A1 - Gartman, Amy A1 - Clarke, David R A1 - Girguis, Peter R. JF - AGU Fall Meeting Abstracts VL - 2014 ER - TY - JOUR T1 - Characterizing the plasticity of nitrogen metabolism by the host and symbionts of the hydrothermal vent chemoautotrophic symbioses Ridgeia piscesae JF - Molecular Ecology Y1 - 2014 A1 - Liao, L. A1 - Wankel, S. D. A1 - Wu, M. A1 - Cavanaugh, C. M. A1 - Girguis, P. R. KW - bacterial endosymbiont KW - de-fuca ridge KW - guaymas basin KW - hydrothermal vent KW - inorganic nitrogen KW - isotope fractionation KW - marine diatoms KW - nitrate reductases KW - nitrogen KW - physiological proteomics KW - ridgeia KW - symbiosis KW - tubeworm KW - tubeworm riftia-pachyptila KW - vestimentiferan tubeworms AB -

Chemoautotrophic symbionts of deep sea hydrothermal vent tubeworms are known to provide their hosts with all their primary nutrition. While studies have examined how chemoautotrophic symbionts provide the association with nitrogen, fewer have examined if symbiont nitrogen metabolism varies as a function of environmental conditions. Ridgeia piscesae tubeworms flourish at Northeastern Pacific vents, occupy a range of microhabitats, and exhibit a high degree of morphological plasticity [e.g. long-skinny (LS) and short-fat (SF) phenotypes] that may relate to environmental conditions. This plasticity affords an opportunity to examine whether symbiont nitrogen metabolism varies among host phenotypes. LS and SF R.piscesae were recovered from the Axial and Main Endeavour Field hydrothermal vents. Nitrate and ammonium were quantified in Ridgeia blood, and the expression of key nitrogen metabolism genes, as well as stable nitrogen isotope ratios, was quantified in host branchial plume and symbiont-containing tissues. Nitrate and ammonium were abundant in the blood of both phenotypes though environmental ammonium concentrations were, paradoxically, lowest among individuals with the highest blood ammonium. Assimilatory nitrate reductase transcripts were always below detection, though in both LS and SF R.piscesae symbionts, we observed elevated expression of dissimilatory nitrate reductase genes, as well as symbiont and host ammonium assimilation genes. Site-specific differences in expression, along with tissue stable isotope analyses, suggest that LS and SF Ridgeia symbionts are engaged in both dissimilatory nitrate reduction and ammonia assimilation to varying degrees. As such, it appears that environmental conditions -not host phenotype-primarily dictates symbiont nitrogen metabolism.

VL - 23 SN - 0962-1083 N1 -

Sp. Iss. SIAc8hiTimes Cited:2Cited References Count:68

JO - Mol EcolMol Ecol ER - TY - JOUR T1 - Electron uptake by iron-oxidizing phototrophic bacteria. JF - Nature Communications Y1 - 2014 A1 - Bose, A. A1 - Gardel, E. J. A1 - Vidoudez, C. A1 - Parra, E. A. A1 - Girguis, P. R. KW - atom exchange KW - co2 fixation KW - electricity KW - metabolism KW - microorganisms KW - oxidation KW - reduction KW - regulatory twist KW - rhodopseudomonas-palustris cga010 KW - tie-1 AB -

Oxidation-reduction reactions underlie energy generation in nearly all life forms. Although most organisms use soluble oxidants and reductants, some microbes can access solid-phase materials as electron-acceptors or -donors via extracellular electron transfer. Many studies have focused on the reduction of solid-phase oxidants. Far less is known about electron uptake via microbial extracellular electron transfer, and almost nothing is known about the associated mechanisms. Here we show that the iron-oxidizing photoautotroph Rhodopseudomonas palustris TIE-1 accepts electrons from a poised electrode, with carbon dioxide as the sole carbon source/electron acceptor. Both electron uptake and ruBisCo form I expression are stimulated by light. Electron uptake also occurs in the dark, uncoupled from photosynthesis. Notably, the pioABC operon, which encodes a protein system essential for photoautotrophic growth by ferrous iron oxidation, influences electron uptake. These data reveal a previously unknown metabolic versatility of photoferrotrophs to use extracellular electron transfer for electron uptake.

VL - 5 SN - 2041-1723 N1 -

Ac6xzTimes Cited:2Cited References Count:39

JO - Nat CommunNat Commun ER - TY - JOUR T1 - Intracellular Oceanospirillales inhabit the gills of the hydrothermal vent snail Alviniconcha with chemosynthetic, gamma-Proteobacterial symbionts JF - Environmental Microbiology Reports Y1 - 2014 A1 - Beinart, R. A. A1 - Nyholm, S. V. A1 - Dubilier, N. A1 - Girguis, P. R. KW - Bacteria KW - coral stylophora-pistillata KW - diversity KW - endosymbionts KW - genus alviniconcha KW - indian-ocean KW - provannidae KW - sea KW - sp nov. KW - targeted oligonucleotide probes AB -

Associations between bacteria from the -Proteobacterial order Oceanospirillales and marine invertebrates are quite common. Members of the Oceanospirillales exhibit a diversity of interactions with their various hosts, ranging from the catabolism of complex compounds that benefit host growth to attacking and bursting host nuclei. Here, we describe the association between a novel Oceanospirillales phylotype and the hydrothermal vent snail Alviniconcha. Alviniconcha typically harbour chemoautotrophic - or epsilon-Proteobacterial symbionts inside their gill cells. Via fluorescence in situ hybridization and transmission electron microscopy, we observed an Oceanospirillales phylotype (named AOP for AlviniconchaOceanospirillales phylotype') in membrane-bound vacuoles that were separate from the known - or epsilon-Proteobacterial symbionts. Using quantitative polymerase chain reaction, we surveyed 181 Alviniconcha hosting -Proteobacterial symbionts and 102 hosting epsilon-Proteobacterial symbionts, and found that the population size of AOP was always minor relative to the canonical symbionts (median 0.53% of the total quantified 16S rRNA genes). Additionally, we detected AOP more frequently in Alviniconcha hosting -Proteobacterial symbionts than in those hosting epsilon-Proteobacterial symbionts (96% and 5% of individuals respectively). The high incidence of AOP in -Proteobacteria hosting Alviniconcha implies that it could play a significant ecological role either as a host parasite or as an additional symbiont with unknown physiological capacities.

VL - 6 SN - 1758-2229 N1 -

Au6guTimes Cited:0Cited References Count:51

JO - Env Microbiol RepEnv Microbiol Rep ER - TY - JOUR T1 - Oxygen, ecology, and the Cambrian radiation of animals JF - Integrative and Comparative Biology Y1 - 2014 A1 - Sperling, E. A. A1 - Frieder, C. A. A1 - Raman, A. V. A1 - Girguis, P. R. A1 - Levin, L. A. A1 - Knoll, A. H. VL - 54 SN - 1540-7063 N1 -

Suppl. 1Ad2fpTimes Cited:0Cited References Count:0

JO - Integr Comp BiolIntegr Comp Biol ER - TY - JOUR T1 - Anaerobic oxidation of short-chain alkanes in hydrothermal sediments: potential influences on sulfur cycling and microbial diversity JF - Frontiers in Microbiology Y1 - 2013 A1 - Adams, Melissa M A1 - Hoarfrost, Adrienne L A1 - Bose, Arpita A1 - Joye, Samantha B A1 - Girguis, Peter R. PB - Frontiers VL - 4 ER - TY - JOUR T1 - Assessing the influence of physical, geochemical and biological factors on anaerobic microbial primary productivity within hydrothermal vent chimneys JF - Geobiology Y1 - 2013 A1 - Olins, H. C. A1 - Rogers, D. R. A1 - Frank, K. L. A1 - Vidoudez, C. A1 - Girguis, P. R. VL - 11 IS - 3 ER - TY - JOUR T1 - Autonomous application of quantitative PCR in the deep sea: in situ surveys of aerobic methanotrophs using the deep-sea environmental sample processor JF - Environmental science & technology Y1 - 2013 A1 - Ussler III, William A1 - Preston, Christina A1 - Tavormina, Patricia A1 - Pargett, Doug A1 - Jensen, Scott A1 - Roman, Brent A1 - Marin III, Roman A1 - Shah, Sunita R A1 - Girguis, Peter R. A1 - Birch, James M A1 - others PB - American Chemical Society VL - 47 IS - 16 ER - TY - ABST T1 - Biochemical systems for sulfur and carbon sequestration Y1 - 2013 A1 - Peter Girguis A1 - Reimers, Clare E N1 - US Patent App. 13/991,785 ER - TY - CONF T1 - Biogeography and diversity of methane and sulfur-cycling ecotypes in deep subsurface sediments T2 - AGU Fall Meeting Abstracts Y1 - 2013 A1 - Adams, M. M. A1 - Biddle, J A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2013 ER - TY - JOUR T1 - Characterizing microbial community and geochemical dynamics at hydrothermal vents using osmotically driven continuous fluid samplers JF - Environmental science & technology Y1 - 2013 A1 - Robidart, Julie A1 - Callister, Stephen J A1 - Song, Pengfei A1 - Nicora, Carrie D A1 - Wheat, Charles G A1 - Girguis, Peter R. PB - American Chemical Society VL - 47 IS - 9 ER - TY - JOUR T1 - Characterizing the distribution and rates of microbial sulfate reduction at Middle Valley hydrothermal vents JF - The ISME journal Y1 - 2013 A1 - Frank, Kiana L A1 - Rogers, Daniel R A1 - Olins, Heather C A1 - Vidoudez, Charles A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 7 IS - 7 ER - TY - JOUR T1 - Characterizing the distribution of methane sources and cycling in the deep sea via in situ stable isotope analysis JF - Environmental science & technology Y1 - 2013 A1 - Wankel, Scott D A1 - Huang, Yi-wen A1 - Manish Gupta A1 - Provencal, Robert A1 - Leen, J Brian A1 - Fahrland, Andrew A1 - Vidoudez, Charles A1 - Girguis, Peter R. PB - American Chemical Society VL - 47 IS - 3 ER - TY - CONF T1 - Deep water instrument for microbial identification, quantification, and archiving T2 - 2013 OCEANS-San Diego Y1 - 2013 A1 - Pargett, Douglas M A1 - Jensen, Scott D A1 - Roman, Brent A A1 - Preston, Christina M A1 - Ussler, William A1 - Girguis, Peter R. A1 - Marin, Roman A1 - Birch, James M A1 - Scholin, Christopher A JF - 2013 OCEANS-San Diego PB - IEEE ER - TY - JOUR T1 - Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments JF - Frontiers in microbiology Y1 - 2013 A1 - Bose, Arpita A1 - Rogers, Daniel R A1 - Adams, Melissa M A1 - Joye, Samantha B A1 - Girguis, Peter R. PB - Frontiers VL - 4 ER - TY - JOUR T1 - In situ chemistry and microbial community compositions in five deep-sea hydrothermal fluid samples from I rina II in the L ogatchev field JF - Environmental Microbiology Y1 - 2013 A1 - Perner, Mirjam A1 - Gonnella, Giorgio A1 - Hourdez, Stephane A1 - Böhnke, Stefanie A1 - Kurtz, Stefan A1 - Peter Girguis VL - 15 IS - 5 ER - TY - JOUR T1 - Low temperature geomicrobiology follows host rock composition along a geochemical gradient in Lau Basin JF - Frontiers in Microbiology Y1 - 2013 A1 - Sylvan, Jason B A1 - Sia, Tiffany Y A1 - Haddad, Amanda G A1 - Briscoe, Lindsey J A1 - Toner, Brandy M A1 - Girguis, Peter R. A1 - Edwards, Katrina J PB - Frontiers VL - 4 ER - TY - JOUR T1 - Metatranscriptomics reveal differences in in situ energy and nitrogen metabolism among hydrothermal vent snail symbionts JF - The ISME journal Y1 - 2013 A1 - Sanders, J. G. A1 - Beinart, R. A. A1 - Stewart, F. J. A1 - Delong, E. F. A1 - Girguis, P. R. PB - Nature Publishing Group VL - 7 IS - 8 ER - TY - CONF T1 - Microbial transformations of carbon in crustal aquifer fluids at North Pond, Mid-Atlantic Ridge T2 - AGU Fall Meeting Abstracts Y1 - 2013 A1 - Girguis, P. R. A1 - Jaekel, U A1 - Dittmar, T A1 - Meyer, JL A1 - Glazer, BT A1 - Huber, JA JF - AGU Fall Meeting Abstracts VL - 2013 ER - TY - JOUR T1 - Oxygen, ecology, and the Cambrian radiation of animals JF - Proceedings of the National Academy of Sciences Y1 - 2013 A1 - Sperling, Erik A A1 - Frieder, Christina A A1 - Raman, Akkur V A1 - Girguis, Peter R. A1 - Levin, Lisa A A1 - Knoll, Andrew H PB - National Academy of Sciences VL - 110 IS - 33 ER - TY - JOUR T1 - Redox effects on the microbial degradation of refractory organic matter in marine sediments JF - Geochimica et Cosmochimica Acta Y1 - 2013 A1 - Reimers, Clare E A1 - Alleau, Yvan A1 - Bauer, James E A1 - Delaney, Jennifer A1 - Girguis, Peter R. A1 - Schrader, Paul S A1 - Stecher III, Hilmar A PB - Pergamon VL - 121 ER - TY - JOUR T1 - Respiration control of multicellularity in Bacillus subtilis by a complex of the cytochrome chain with a membrane-embedded histidine kinase JF - Genes & development Y1 - 2013 A1 - Kolodkin-Gal, Ilana A1 - Elsholz, Alexander KW A1 - Muth, Christine A1 - Girguis, Peter R. A1 - Kolter, Roberto A1 - Losick, Richard PB - Cold Spring Harbor Lab VL - 27 IS - 8 ER - TY - CONF T1 - Sensors and acoustic modems powered by Benthic Microbial Fuel Cells at the MARS observatory T2 - 2013 OCEANS-San Diego Y1 - 2013 A1 - Schrader, Paul S A1 - Cody Doolan A1 - Reimers, Clare E A1 - Girguis, Peter R. A1 - Michael Wolf A1 - Dale Green JF - 2013 OCEANS-San Diego PB - IEEE ER - TY - JOUR T1 - Workshop on telepresence-enabled exploration of the Caribbean region JF - Oceanography Y1 - 2013 A1 - Bell, Katherine LC A1 - Brennan, Michael L A1 - Peter Girguis A1 - Austin Jr, James A A1 - Carey, Steven N A1 - Coleman, Dwight F A1 - Mayer, Larry A1 - Ballard, Robert D A1 - Martinez, Catalina A1 - Russell, Craig PB - OCEANOGRAPHY SOC PO BOX 1931, ROCKVILLE, MD USA VL - 26 IS - 1 ER - TY - JOUR T1 - Capitalizing on Expertise in Marine Education and Outreach to Broaden Impacts. JF - The Journal of Marine Education Y1 - 2013 A1 - Girguis, P. R. A1 - DeCharon, A. A1 - Herren, C.M. VL - 28 IS - 3 ER - TY - Generic T1 - The Science and Applications of Microbial Genomics Y1 - 2013 A1 - Girguis, P. R. A1 - Choffnes, E.R. A1 - LeighAnne, O. A1 - Wizemann, T.M. PB - The National Academies Press CY - Washington, D.C. N1 -

The

ER - TY - JOUR T1 - Anaerobic oxidation of short-chain alkanes in hydrothermal sediments: potential influences on sulfur cycling and microbial diversity JF - Frontiers in Microbiology Y1 - 2013 A1 - Adams, M. M. A1 - Hoarfrost, A. L. A1 - Bose, A. A1 - Joye, S. B. A1 - Girguis, P. R. KW - cold seeps KW - de-fuca ridge KW - gas hydrate KW - gulf-of-mexico KW - hydrothermal vent KW - intrinsic bioremediation KW - juan de fuca ridge KW - marine methane oxidation KW - metalliferous sediments KW - middle valley KW - molecular-weight hydrocarbons KW - short-chain alkanes KW - sulfate reduction KW - vent fluids AB -

Short-chain alkanes play a substantial role in carbon and sulfur cycling at hydrocarbon-rich environments globally, yet few studies have examined the metabolism of ethane (C-2), propane (C-3), and butane (C-4) in anoxic sediments in contrast to methane (C-1). In hydrothermal vent systems, short-chain alkanes are formed over relatively short geological time scales via thermogenic processes and often exist at high concentrations. The sediment-covered hydrothermal vent systems at Middle Valley (MV Juan de Fuca Ridge) are an ideal site for investigating the anaerobic oxidation of C-1-C-4 alkanes, given the elevated temperatures and dissolved hydrocarbon species characteristic of these metalliferous sediments. We examined whether MV microbial communities oxidized C-1-C-4 alkanes under mesophilic to thermophilic sulfate-reducing conditions. Here we present data from discrete temperature (25, 55, and 75 degrees C) anaerobic batch reactor incubations of MV sediments supplemented with individual alkanes. Co-registered alkane consumption and sulfate reduction (SR) measurements provide clear evidence for C-1-C-4 alkane oxidation linked to SR over time and across temperatures. In these anaerobic batch reactor sediments, 16S ribosomal RNA pyrosequencing revealed that Deltaproteobacteria, particularly a novel sulfate-reducing lineage, were the likely phylotypes mediating the oxidation of C-2-C-4 alkanes. Maximum C-1-C-4 alkane oxidation rates occurred at 55 degrees C, which reflects the mid-core sediment temperature profile and corroborates previous studies of rate maxima for the anaerobic oxidation of methane (AOM). Of the alkanes investigated, C-3 was oxidized at the highest rate over time, then C-4, C-2, and C-1, respectively. The implications of these results are discussed with respect to the potential competition between the anaerobic oxidation of C-2-C(4)alkanes with AOM for available oxidants and the influence on the fate of C-1 derived from these hydrothermal systems.

VL - 4 SN - 1664-302X N1 -

Aa5buTimes Cited:5Cited References Count:58

JO - Front MicrobiolFront Microbiol ER - TY - JOUR T1 - Assessing the influence of physical, geochemical and biological factors on anaerobic microbial primary productivity within hydrothermal vent chimneys. JF - Geobiology Y1 - 2013 A1 - Olins, H. C. A1 - Rogers, D. R. A1 - Frank, K. L. A1 - Vidoudez, C. A1 - Girguis, P. R. KW - autotrophic carbon fixation KW - chemoautotrophic symbionts KW - de-fuca ridge KW - east pacific rise KW - epsilon-proteobacteria KW - flank crustal fluids KW - low-temperature KW - mid-atlantic ridge KW - riftia-pachyptila KW - sulfur-oxidizing chemolithoautotroph AB -

Chemosynthetic primary production supports hydrothermal vent ecosystems, but the extent of that productivity and its governing factors have not been well constrained. To better understand anaerobic primary production within massive vent deposits, we conducted a series of incubations at 4, 25, 50 and 90 degrees C using aggregates recovered from hydrothermal vent structures. We documented in situ geochemistry, measured autochthonous organic carbon stable isotope ratios and assessed microbial community composition and functional gene abundances in three hydrothermal vent chimney structures from Middle Valley on the Juan de Fuca Ridge. Carbon fixation rates were greatest at lower temperatures and were comparable among chimneys. Stable isotope ratios of autochthonous organic carbon were consistent with the CalvinBensonBassham cycle being the predominant mode of carbon fixation for all three chimneys. Chimneys exhibited marked differences in vent fluid geochemistry and microbial community composition, with structures being differentially dominated by gamma () or epsilon (epsilon) proteobacteria. Similarly, qPCR analyses of functional genes representing different carbon fixation pathways showed striking differences in gene abundance among chimney structures. Carbon fixation rates showed no obvious correlation with observed in situ vent fluid geochemistry, community composition or functional gene abundance. Together, these data reveal that (i) net anaerobic carbon fixation rates among these chimneys are elevated at lower temperatures, (ii) clear differences in community composition and gene abundance exist among chimney structures, and (iii) tremendous spatial heterogeneity within these environments likely confounds efforts to relate the observed rates to in situ microbial and geochemical factors. We also posit that microbes typically thought to be mesophiles are likely active and growing at cooler temperatures, and that their activity at these temperatures comprises the majority of endolithic anaerobic primary production in hydrothermal vent chimneys.

VL - 11 SN - 1472-4677 N1 -

123ZATimes Cited:1Cited References Count:101

JO - GeobiologyGeobiology ER - TY - JOUR T1 - Autonomous Application of Quantitative PCR in the Deep Sea: In Situ Surveys of Aerobic Methanotrophs Using the Deep-Sea Environmental Sample Processor JF - Environmental Science & Technology Y1 - 2013 A1 - Ussler, W. A1 - Preston, C. A1 - Tavormina, P. A1 - Pargett, D. A1 - Jensen, S. A1 - Roman, B. A1 - Marin, R. A1 - Shah, S. R. A1 - Girguis, P. R. A1 - Birch, J. M. A1 - Orphan, V. A1 - Scholin, C. KW - esp KW - harmful algae KW - oxygen minimum zone KW - remote detection AB -

Recent advances in ocean observing systems and genomic technologies have led to the development of the deep-sea environmental sample processor (D-ESP). The DESP filters particulates from seawater at depths up to 4000 m and applies a variety of molecular assays to the particulates, including quantitative PCR (qPCR), to identify particular organisms and genes in situ. Preserved samples enable laboratory-based validation of in situ results and expanded studies of genomic diversity and gene expression. Tests of the D-ESP at a methane-rich mound in the Santa Monica Basin centered on detection of 16S rRNA and particulate methane monooxygenase (pmoA) genes for two putative aerobic methanotrophs. Comparison of in situ qPCR results with laboratory-based assays of preserved samples demonstrates the D-ESP generated high-quality qPCR data while operating autonomously on the seafloor. Levels of 16S rRNA and pmoA cDNA detected in preserved samples are consistent with an active community of aerobic methanotrophs near the methane-rich mound. These findings are substantiated at low methane sites off Point Conception and in Monterey Bay where target genes are at or below detection limits. Successful deployment of the D-ESP is a major step toward developing autonomous systems to facilitate a wide range of marine microbiological investigations.

VL - 47 SN - 0013-936X N1 -

205VATimes Cited:2Cited References Count:24

JO - Environ Sci TechnolEnviron Sci Technol ER - TY - JOUR T1 - Characterizing Microbial Community and Geochemical Dynamics at Hydrothermal Vents Using Osmotically Driven Continuous Fluid Samplers JF - Environmental Science & Technology Y1 - 2013 A1 - Robidart, J. A1 - Callister, S. J. A1 - Song, P. F. A1 - Nicora, C. D. A1 - Wheat, C. G. A1 - Girguis, P. R. KW - bacterial KW - diversity KW - dominance KW - field KW - growth KW - high-resolution KW - mid-atlantic ridge KW - population-dynamics KW - quantification AB -

Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for >1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS's potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.

VL - 47 SN - 0013-936X N1 -

141WATimes Cited:2Cited References Count:36

JO - Environ Sci TechnolEnviron Sci Technol ER - TY - JOUR T1 - Characterizing the distribution and rates of microbial sulfate reduction at Middle Valley hydrothermal vents. JF - ISME Journal Y1 - 2013 A1 - Frank, K. L. A1 - Rogers, D. R. A1 - Olins, H. C. A1 - Vidoudez, C. A1 - Girguis, P. R. KW - anaerobic oxidation KW - dissolved organic-carbon KW - epsilon-proteobacteria KW - estuarine sediments KW - guaymas basin KW - hydrothermal vent KW - microbial ecology KW - molecular characterization KW - phylogenetic diversity KW - primary productivity KW - reducing bacteria KW - ribosomal-rna analysis KW - sp-nov. KW - sulfate reduction AB -

Few studies have directly measured sulfate reduction at hydrothermal vents, and relatively little is known about how environmental or ecological factors influence rates of sulfate reduction in vent environments. A better understanding of microbially mediated sulfate reduction in hydrothermal vent ecosystems may be achieved by integrating ecological and geochemical data with metabolic rate measurements. Here we present rates of microbially mediated sulfate reduction from three distinct hydrothermal vents in the Middle Valley vent field along the Juan de Fuca Ridge, as well as assessments of bacterial and archaeal diversity, estimates of total biomass and the abundance of functional genes related to sulfate reduction, and in situ geochemistry. Maximum rates of sulfate reduction occurred at 90 degrees C in all three deposits. Pyrosequencing and functional gene abundance data revealed differences in both biomass and community composition among sites, including differences in the abundance of known sulfate-reducing bacteria. The abundance of sequences for Thermodesulfovibro-like organisms and higher sulfate reduction rates at elevated temperatures suggests that Thermodesulfovibro-like organisms may have a role in sulfate reduction in warmer environments. The rates of sulfate reduction presented here suggest that-within anaerobic niches of hydrothermal deposits-heterotrophic sulfate reduction may be quite common and might contribute substantially to secondary productivity, underscoring the potential role of this process in both sulfur and carbon cycling at vents.

VL - 7 SN - 1751-7362 N1 -

170LMTimes Cited:5Cited References Count:70

JO - Isme JIsme J ER - TY - JOUR T1 - Characterizing the Distribution of Methane Sources and Cycling in the Deep Sea via in Situ Stable Isotope Analysis JF - Environmental Science & Technology Y1 - 2013 A1 - Wankel, S. D. A1 - Huang, Y. W. A1 - Gupta, M. A1 - Provencal, R. A1 - Leen, J. B. A1 - Fahrland, A. A1 - Vidoudez, C. A1 - Girguis, P. R. KW - anaerobic oxidation KW - carbon-dioxide KW - cavity-output spectroscopy KW - dissolved-gases KW - inlet mass-spectrometry KW - laser absorption spectrometer KW - monterey-bay KW - n2o isotopomers KW - organic-compounds KW - quantum-cascade laser AB -

The capacity to make in situ geo-referenced measurements of methane concentration and stable isotopic composition (delta C-13(CH4)) would greatly improve our understanding of the distribution and type of methane sources in the environment, allow refined determination of the extent to which microbial production and consumption contributes to methane cycling, and enable the testing of hypotheses about the sensitivity of methane cycling to changes in environmental conditions. In particular, characterizing biogeochemical methane cycling dynamics in the deep ocean is hampered by a number of challenges, especially in environments where high methane concentrations preclude intact recovery of undisturbed samples. To that end, we have developed an in situ analyzer capable of delta C-13(CH4) measurements in the deep ocean. Here we present data from laboratory and field studies in which we characterize the instrument's analytical capabilities and performance and provide the first in situ stable isotope based characterization of the influence of anaerobic methane oxidation on methane flux from seep sediments. These data illustrate how in situ measurements can permit finer-scale analyses of variations in AOM activity, and facilitate advances in using delta C-13(CH4) and other isotopic systems to interrogate biogeochemical cycles in the deep sea and other remote or challenging environments.

VL - 47 SN - 0013-936X N1 -

086HUTimes Cited:4Cited References Count:54

JO - Environ Sci TechnolEnviron Sci Technol ER - TY - Generic T1 - Deep Water Instrument for Microbial Identification, Quantification, and Archiving T2 - 2013 Oceans - San Diego Y1 - 2013 A1 - Pargett, D. M. A1 - Jensen, S. D. A1 - Roman, B. A. A1 - Preston, C. M. A1 - Ussler, W. A1 - Girguis, P. R. A1 - Marin, R. A1 - Birch, J. M. A1 - Scholin, C. A. KW - autonomous sampling KW - deep ocean KW - ecogenomic sensor KW - harmful algae KW - in situ instrumentation KW - remote detection KW - sample processor esp AB -

A deep ocean robotic platform capable of in situ microbial identification and quantification at depths to 4000 m has been developed. The platform is a free benthic lander, containing a low pressure microbial instrument and deep ocean sampling systems that can monitor and collect raw seawater from multiple sources, then decompress the raw seawater for processing by the detection instrument. This has enabled the autonomous collection, processing, and archiving of microbes from multiple sites. This allows for direct, time correlated comparison of different microbial populations along with the seawater physical and chemical composition.

JF - 2013 Oceans - San Diego SN - 0197-7385 N1 -

Ba3erTimes Cited:0Cited References Count:8Oceans-Ieee

JO - Oceans-IeeeOceans-Ieee ER - TY - JOUR T1 - Geomicrobiological linkages between short-chain alkane consumption and sulfate reduction rates in seep sediments JF - Frontiers in Microbiology Y1 - 2013 A1 - Bose, A. A1 - Rogers, D. R. A1 - Adams, M. M. A1 - Joye, S. B. A1 - Girguis, P. R. KW - 16s ribosomal-rna KW - anaerobic oxidation KW - butane KW - c-1-c-4 hydrocarbons KW - cold seeps KW - ethane KW - gas hydrate KW - gulf of mexico KW - gulf-of-mexico KW - methane KW - methane oxidation KW - microbial diversity KW - microbial sulfate reduction KW - phylogenetic analysis KW - propane KW - reducing bacteria KW - short-chain alkanes KW - spectrophotometric determination AB -

Marine hydrocarbon seeps are ecosystems that are rich in methane, and, in some cases, short-chain (C-2-C-5) and longer alkanes. C-2-C-4 alkanes such as ethane, propane, and butane can be significant components of seeping fluids. Some sulfate-reducing microbes oxidize short-chain alkanes anaerobically, and may play an important role in both the competition for sulfate and the local carbon budget. To better understand the anaerobic oxidation of short-chain n-alkanes coupled with sulfate-reduction, hydrocarbon-rich sediments from the Gulf of Mexico (GoM) were amended with artificial, sulfate-replete seawater and one of four n-alkanes (C-1-C-4) then incubated under strict anaerobic conditions. Measured rates of alkane oxidation and sulfate reduction closely follow stoichiometric predictions that assume the complete oxidation of alkanes to CO2 (though other sinks for alkane carbon likely exist). Changes in the delta C-13 of all the alkanes in the reactors show enrichment over the course of the incubation, with the C-3 and C-4 incubations showing the greatest enrichment (4.4 and 4.5 parts per thousand, respectively). The concurrent depletion in the delta C-13 of dissolved inorganic carbon (DIC) implies a transfer of carbon from the alkane to the DIC pool (-3.5 and -6.7 parts per thousand for C-3 and C-4 incubations, respectively). Microbial community analyses reveal that certain members of the class Deltaproteobacteria are selectively enriched as the incubations degrade C-1-C-4 alkanes. Phylogenetic analyses indicate that distinct phylotypes are enriched in the ethane reactors, while phylotypes in the propane and butane reactors align with previously identified C-3-C-4 alkane-oxidizing sulfate-reducers. These data further constrain the potential influence of alkane oxidation on sulfate reduction rates (SRRs) in cold hydrocarbon-rich sediments, provide insight into their contribution to local carbon cycling, and illustrate the extent to which short-chain alkanes can serve as electron donors and govern microbial community composition and density.

VL - 4 SN - 1664-302X N1 -

Ab1kqTimes Cited:4Cited References Count:53

JO - Front MicrobiolFront Microbiol ER - TY - JOUR T1 - Low temperature geomicrobiology follows host rock composition along a geochemical gradient in Lau Basin JF - Frontiers in Microbiology Y1 - 2013 A1 - Sylvan, J. B. A1 - Sia, T. Y. A1 - Haddad, A. G. A1 - Briscoe, L. J. A1 - Toner, B. M. A1 - Girguis, P. R. A1 - Edwards, K. J. KW - back-arc basin KW - bacteroidetes KW - basalt KW - community diversity KW - deep-sea KW - east pacific rise KW - epsilon-proteobacteria KW - geomicrobiology KW - hydrothermal KW - hydrothermal plume KW - inactive sulfides KW - microbial diversity KW - oxidizing bacteria KW - sea-floor basalt KW - sp-nov. AB -

The East Lau Spreading Center (ELSC) and Valu Fa Ridge (VFR) comprise a ridge segment in the southwest Pacific Ocean where rapid transitions in the underlying mantle chemistry manifest themselves as gradients in seafloor rock geochemistry. We studied the geology and microbial diversity of three silicate rock samples and three inactive sulfide chimney samples collected, from north to south, at the vent fields Kilo Moana, ABE, Tui Malila, and Mariner. This is the first study of microbial populations on basaltic andesite, which was sampled at Mariner vent field. Silicate rock geochemistry exhibits clear latitudinal trends that are mirrored by changes in bacterial community composition. alpha-proteobacteria, epsilon-proteobacteria, and Bacteroidetes are most common on a silicate collected from Kilo Moana and their proportions decrease linearly on silicates collected further south. Conversely, a silicate from Mariner vent field hosts high proportions of a unique lineage of Chloroflexi unrelated (<90% sequence similarity) to previously recovered environmental clones or isolates, which decrease at ABE and are absent at Kilo Moana. The exteriors of inactive sulfide structures are dominated by lineages of sulfur oxidizing alpha-proteobacteria, gamma-proteobacteria, and epsilon-proteobacteria, while the interior of one chimney is dominated by putative sulfur-reducing delta-proteobacteria. A comparison of bacterial communities on inactive sulfides from this and previous studies reveals the presence of a clade of uncultured Bacteroidetes exclusive to sulfidic environments, and a high degree of heterogeneity in bacterial community composition from one sulfide structure to another. In light of the heterogeneous nature of bacterial communities observed here and in previous studies of both active and inactive hydrothermal sulfide structures, the presence of numerous niches may be detected on these structures in the future by finer scale sampling and analysis.

VL - 4 SN - 1664-302X N1 -

Aa3pxTimes Cited:2Cited References Count:60

JO - Front MicrobiolFront Microbiol ER - TY - JOUR T1 - Metatranscriptomics reveal differences in in situ energy and nitrogen metabolism among hydrothermal vent snail symbionts JF - ISME Journal Y1 - 2013 A1 - Sanders, J. G. A1 - Beinart, R. A. A1 - Stewart, F. J. A1 - Delong, E. F. A1 - Girguis, P. R. KW - alviniconcha KW - Bacteria KW - calyptogena-magnifica KW - chemoautotrophy KW - deep-sea vents KW - escherichia-coli KW - gene-expression KW - genome KW - hydrothermal vents KW - metatranscriptomics KW - nitrate respiration KW - riftia-pachyptila endosymbiont KW - sulfurimonas-denitrificans KW - symbiosis KW - tube worm AB -

Despite the ubiquity of chemoautotrophic symbioses at hydrothermal vents, our understanding of the influence of environmental chemistry on symbiont metabolism is limited. Transcriptomic analyses are useful for linking physiological poise to environmental conditions, but recovering samples from the deep sea is challenging, as the long recovery times can change expression profiles before preservation. Here, we present a novel, in situ RNA sampling and preservation device, which we used to compare the symbiont metatranscriptomes associated with Alviniconcha, a genus of vent snail, in which specific host-symbiont combinations are predictably distributed across a regional geochemical gradient. Metatranscriptomes of these symbionts reveal key differences in energy and nitrogen metabolism relating to both environmental chemistry (that is, the relative expression of genes) and symbiont phylogeny (that is, the specific pathways employed). Unexpectedly, dramatic differences in expression of transposases and flagellar genes suggest that different symbiont types may also have distinct life histories. These data further our understanding of these symbionts' metabolic capabilities and their expression in situ, and suggest an important role for symbionts in mediating their hosts' interaction with regional-scale differences in geochemistry.

VL - 7 SN - 1751-7362 N1 -

187LFTimes Cited:6Cited References Count:67

JO - Isme JIsme J ER - TY - JOUR T1 - Oxygen, ecology, and the Cambrian radiation of animals JF - Proceedings of the National Academy of Sciences of the United States of America Y1 - 2013 A1 - Sperling, E. A. A1 - Frieder, C. A. A1 - Raman, A. V. A1 - Girguis, P. R. A1 - Levin, L. A. A1 - Knoll, A. H. KW - bathyal macrofauna KW - Community structure KW - continental-margin KW - diversity KW - ediacaran KW - Evolution KW - hypoxia KW - life KW - metazoa KW - minimum zone KW - organic-matter KW - origin AB -

The Proterozoic-Cambrian transition records the appearance of essentially all animal body plans (phyla), yet to date no single hypothesis adequately explains both the timing of the event and the evident increase in diversity and disparity. Ecological triggers focused on escalatory predator-prey "arms races" can explain the evolutionary pattern but not its timing, whereas environmental triggers, particularly ocean/atmosphere oxygenation, do the reverse. Using modern oxygen minimum zones as an analog for Proterozoic oceans, we explore the effect of low oxygen levels on the feeding ecology of polychaetes, the dominant macrofaunal animals in deep-sea sediments. Here we show that low oxygen is clearly linked to low proportions of carnivores in a community and low diversity of carnivorous taxa, whereas higher oxygen levels support more complex food webs. The recognition of a physiological control on carnivory therefore links environmental triggers and ecological drivers, providing an integrated explanation for both the pattern and timing of Cambrian animal radiation.

VL - 110 SN - 0027-8424 IS - 33 N1 -

200LATimes Cited:13Cited References Count:57

JO - P Natl Acad Sci USAP Natl Acad Sci USA ER - TY - JOUR T1 - Redox effects on the microbial degradation of refractory organic matter in marine sediments JF - Geochimica Et Cosmochimica Acta Y1 - 2013 A1 - Reimers, C. E. A1 - Alleau, Y. A1 - Bauer, J. E. A1 - Delaney, J. A1 - Girguis, P. R. A1 - Schrader, P. S. A1 - Stecher, H. A. KW - carbon preservation KW - communities KW - continental-margin sediments KW - energy KW - extracellular electron-transfer KW - fuel-cells KW - hydrogen sulfide KW - oxygen KW - sea-floor KW - shelf sediments AB -

Microbially mediated reduction-oxidation (redox) reactions are often invoked as being the mechanisms by which redox state influences the degradation of sedimentary organic matter (OM) in the marine environment. To evaluate the effects of elevated, oscillating and reduced redox potentials on the fate of primarily aged, mineral-adsorbed OM contained in continental shelf sediments, we used microbial fuel cells to control redox state within and around marine sediments, without amending the sediments with reducing or oxidizing substances. We subsequently followed electron fluxes in the redox elevated and redox oscillating treatments, and related sediment chemical, isotopic and bacterial community changes to redox conditions over a 748-day experimental period.The electron fluxes of the elevated and oscillating redox cells were consistent with models of organic carbon (OC) oxidation with time-dependent first-order rate constants declining from 0.023 to 0.005 y(-1), in agreement with rate constants derived from typical OC profiles and down core ages of offshore sediments, or from sulfate reduction rate measurements in similar sediments. Moreover, although cumulative electron fluxes were higher in the continuously elevated redox treatment, incremental rates of electron harvesting in the two treatments converged over the 2 year experiment. These similar rates were reflected in chemical indicators of OM metabolism such as dissolved OC and ammonia, and particulate OC concentrations, which were not significantly different among all treatments and controls over the experimental time-scale. In contrast, products of carbonate and opal dissolution and metal mobilization showed greater enrichments in sediments with elevated and oscillating redox states.Microbial community composition in anode biofilms and surrounding sediments was assessed via high-throughput 16S rRNA gene sequencing, and these analyses revealed that the elevated and oscillatory redox treatments led to the enrichment of Deltaproteobacteria on the sediment-hosted anodes over time. Many Deltaproteobacteria are capable of using electrodes as terminal electron acceptors to completely oxidize organic substrates. Notably, Deltaproteobacteria were not measurably enriched in the sediments adjacent to anodes, suggesting that - in these experiments - electron-shuttling bacterial networks did not radiate out away from the electrodes, affecting millimeters or centimeters of sediment. Rather, microbial phylotypes allied to the Clostridia appeared to dominate in the sediment amongst all treatments, and likely played essential roles in converting complex dissolved and particulate sources of OM to simple fermentation products. Thus, we advance that the rate at which fermentation products are generated and migrate to oxidation fronts is what limits the remineralization of OM in many subsurface sediments removed from molecular oxygen. This is a diagenetic scenario that is consistent with the discharging behavior of redox oscillating sediment MFCs. It is also compatible with hypotheses that molecular O-2 - and not just the resulting elevated redox potential - may be required to effectively catalyze the degradation of refractory OM. Such decomposition reactions have been suggested to depend on substrate interactions with highly reactive oxygen- containing radicals and/or with specialized extracellular enzymes produced by aerobic prokaryotic or eukaryotic cells. (c) 2013 Elsevier Ltd. All rights reserved.

VL - 121 SN - 0016-7037 N1 -

226ZRTimes Cited:0Cited References Count:91

JO - Geochim Cosmochim AcGeochim Cosmochim Ac ER - TY - JOUR T1 - Respiration control of multicellularity in Bacillus subtilis by a complex of the cytochrome chain with a membrane-embedded histidine kinase JF - Genes & Development Y1 - 2013 A1 - Kolodkin-Gal, I. A1 - Elsholz, A. K. W. A1 - Muth, C. A1 - Girguis, P. R. A1 - Kolter, R. A1 - Losick, R. KW - activation KW - amyloid fibers KW - Bacillus subtilis KW - biofilm formation KW - biofilms KW - cytochromes KW - gene-expression KW - histidine kinase KW - initiation KW - master regulator KW - phosphorelay KW - Respiration KW - saccharomyces-cerevisiae KW - sensor kinase KW - sporulation AB -

Bacillus subtilis forms organized multicellular communities known as biofilms wherein the individual cells are held together by a self-produced extracellular matrix. The environmental signals that promote matrix synthesis remain largely unknown. We discovered that one such signal is impaired respiration. Specifically, high oxygen levels suppressed synthesis of the extracellular matrix. In contrast, low oxygen levels, in the absence of an alternative electron acceptor, led to increased matrix production. The response to impaired respiration was blocked in a mutant lacking cytochromes caa(3) and bc and markedly reduced in a mutant lacking kinase KinB. Mass spectrometry of proteins associated with KinB showed that the kinase was in a complex with multiple components of the aerobic respiratory chain. We propose that KinB is activated via a redox switch involving interaction of its second transmembrane segment with one or more cytochromes under conditions of reduced electron transport. In addition, a second kinase (KinA) contributes to the response to impaired respiration. Evidence suggests that KinA is activated by a decrease in the nicotinamide adenine dinucleotide (NAD(+))/NADH ratio via binding of NAD(+) to the kinase in a PAS domain A-dependent manner. Thus, B. subtilis switches from a unicellular to a multicellular state by two pathways that independently respond to conditions of impaired respiration.

VL - 27 SN - 0890-9369 N1 -

134NBTimes Cited:11Cited References Count:61

JO - Gene DevGene Dev ER - TY - JOUR T1 - Sensors and Acoustic Modems Powered by Benthic Microbial Fuel Cells at the MARS Observatory JF - 2013 Oceans - San Diego Y1 - 2013 A1 - Schrader, P. S. A1 - Doolan, C. A1 - Reimers, C. E. A1 - Girguis, P. R. A1 - Wolf, M. A1 - Green, D. KW - benthic microbial fuel cells (bmfcs) KW - deep water deployment KW - electricity KW - electrodes KW - energy KW - energy production KW - geobacter-sulfurreducens KW - microorganisms KW - monterey accelerated research system (mars) observatory AB -

The goals of this project were three-fold: 1) to power underwater instruments with energy harvested from deep sea, organic poor sediments using Benthic Microbial Fuel Cells (BMFCs); 2) to relay instrument data acoustically -and in near-real time-through a cabled seafloor observatory or surface vessel, as appropriate; and 3) to characterize the operational performance of these interactive systems during in situ deployments. Two BMFC-underwater sensing/communications packages were demonstrated near the Monterey Accelerated Research System (MARS) observatory in Monterey Bay, California. MARS is a cabled observatory that resides in deep water (similar to 890 m), about 37 km (23 miles) seaward of the Monterey Bay Aquarium Research Institute (MBARI).The BMFCs were constructed using a cylindrical chamber design with a 0.28 m(2) footprint. Each BMFC was used to power an Aanderaa dissolved O-2/temperature or conductivity/temperature sensor, as well as a Teledyne Benthos compact acoustic modem which contained an integrated power management platform (PMP) for the complete system. The packages were deployed from a surface vessel and allowed to descend freely to the seafloor, at locations approximately 0.5 km away from the MARS node, at depths of 863 and 895 m. The PMPs were programmed to record data from both the sensors and the BMFC (whole cell voltage, capacitor voltage, and battery voltage) on an hourly basis, and to monitor overall microbial fuel cell energy production on a daily basis. Post-deployment, BMFC 1 generated a net surplus of energy from days 98 through 166, and remained operational for 210 days. BMFC 2 began generating a surplus of energy on day 54 and remained operational for 158 days. Data recovered from the oceanographic sensors was transmitted acoustically over both the MARS node and to a research vessel, underscoring the utility of this technology.

SN - 0197-7385 N1 -

Ba3erTimes Cited:0Cited References Count:14Oceans-Ieee

JO - Oceans-IeeeOceans-Ieee ER - TY - JOUR T1 - Anaerobic methane oxidation in metalliferous hydrothermal sediments: influence on carbon flux and decoupling from sulfate reduction JF - Environmental microbiology Y1 - 2012 A1 - Wankel, Scott D A1 - Adams, Melissa M A1 - Johnston, David T A1 - Hansel, Colleen M A1 - Joye, Samantha B A1 - Girguis, Peter R. PB - Blackwell Publishing Ltd Oxford, UK VL - 14 IS - 10 ER - TY - CONF T1 - Being There & Getting Back Again: Half a Century of Deep Ocean Research & Discovery with the Human Occupied Vehicle" Alvin" T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - German, CR A1 - Fornari, DJ A1 - Fryer, P A1 - Girguis, P. R. A1 - Humphris, S. E. A1 - Kelley, DS A1 - Tivey, M A1 - Van Dover, C. L. A1 - Von Damm, K JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - CONF T1 - Distribution, activity and function of short-chain alkane degrading phylotypes in hydrothermal vent sediments T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Adams, M. M. A1 - Joye, S. B. A1 - Hoarfrost, A A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - JOUR T1 - Duty cycling influences current generation in multi-anode environmental microbial fuel cells JF - Environmental science & technology Y1 - 2012 A1 - Gardel, Emily J A1 - Nielsen, Mark E A1 - Grisdela Jr, Phillip T A1 - Girguis, Peter R. PB - American Chemical Society VL - 46 IS - 9 ER - TY - JOUR T1 - Evidence for the role of endosymbionts in regional-scale habitat partitioning by hydrothermal vent symbioses JF - Proceedings of the National Academy of Sciences Y1 - 2012 A1 - Beinart, Roxanne A A1 - Sanders, Jon G A1 - Faure, Baptiste A1 - Sylva, Sean P A1 - Lee, Raymond W A1 - Becker, Erin L A1 - Gartman, Amy A1 - Luther, George W A1 - Seewald, Jeffrey S A1 - Fisher, Charles R A1 - others PB - National Academy of Sciences VL - 109 IS - 47 ER - TY - CONF T1 - Exploring the limit of metazoan thermal tolerance via comparative proteomics: Thermally induced expression shifts in hydrothermal vent polychaetes P. sulfincola and P. palmiformis T2 - INTEGRATIVE AND COMPARATIVE BIOLOGY Y1 - 2012 A1 - Dilly, G. F. A1 - Young, C. R. A1 - Lane, W. S. A1 - Pangalinan, J A1 - Girguis, P. R. JF - INTEGRATIVE AND COMPARATIVE BIOLOGY PB - OXFORD UNIV PRESS INC JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA VL - 52 ER - TY - JOUR T1 - Exploring the limit of metazoan thermal tolerance via comparative proteomics: thermally induced changes in protein abundance by two hydrothermal vent polychaetes JF - Proceedings of the Royal Society B: Biological Sciences Y1 - 2012 A1 - Dilly, Geoffrey F A1 - Young, C Robert A1 - Lane, William S A1 - Pangilinan, Jasmyn A1 - Girguis, Peter R. PB - The Royal Society VL - 279 IS - 1741 ER - TY - JOUR T1 - Expression and putative function of innate immunity genes under in situ conditions in the symbiotic hydrothermal vent tubeworm Ridgeia piscesae JF - PloS one Y1 - 2012 A1 - Nyholm, Spencer V. A1 - Song, Pengfei A1 - Dang, Jeanne A1 - Bunce, Corey A1 - Girguis, Peter R. PB - Public Library of Science VL - 7 IS - 6 ER - TY - JOUR T1 - Expression and Putative Function of Innate Immunity Genes under in situ Conditions in the Y1 - 2012 A1 - Nyholm, S. V. A1 - Song, P. A1 - Dang, J A1 - Bunce, C. A1 - Girguis, P. R. ER - TY - CONF T1 - Isotopic Approaches to Allying Productivity and Sulfur Metabolism in Three Symbiotic Hydrothermal Vent Molluscs T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Beinart, R. A1 - Gartman, A. A1 - Sanders, J. G. A1 - Luther, G. W. A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - CONF T1 - Key factors influencing rates of heterotrophic sulfate reduction in hydrothermal massive sulfide deposits T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Frank, K. L. A1 - Rogers, D A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - CONF T1 - Linking hydrothermal plume geochemistry with deep-sea microbial community structure along the Eastern Lau Spreading Center T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Breier, JA A1 - Toner, B. M. A1 - Sheik, C A1 - Anantharaman, K A1 - Sylvan, J. B. A1 - Edwards, K. J. A1 - Girguis, P. R. A1 - Wendt, K A1 - Sorensen, J A1 - Madison, A A1 - others JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - JOUR T1 - Links from mantle to microbe at the Lau Integrated Study Site: Insights from a back-arc spreading center JF - Oceanography Y1 - 2012 A1 - Tivey, Margaret K A1 - Becker, Erin A1 - Beinart, Roxanne A1 - Fisher, Charles R A1 - Girguis, Peter R. A1 - Langmuir, Charles H A1 - Michael, Peter J A1 - Reysenbach, Anna-Louise PB - The Oceanography Society VL - 25 IS - 1 ER - TY - CONF T1 - Microbial life in cold, hydrologically active oceanic crustal fluids T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Meyer, JL A1 - Jaekel, U A1 - Girguis, P. R. A1 - Glazer, BT A1 - Huber, JA JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - CONF T1 - Microbial Primary Productivity in Hydrothermal Vent Chimneys at Middle Valley, Juan de Fuca Ridge T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Olins, H. C. A1 - Rogers, D A1 - Frank, K. L. A1 - Girguis, P. R. A1 - Vidoudez, C. JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - JOUR T1 - On the potential for bioenergy and biofuels from hydrothermal vent microbes JF - Oceanography Y1 - 2012 A1 - Girguis, Peter R. A1 - Holden, James F PB - Oceanography Society VL - 25 IS - 1 ER - TY - CONF T1 - Transformations of organic matter in the deep biosphere at North Pond T2 - AGU Fall Meeting Abstracts Y1 - 2012 A1 - Jaekel, U A1 - Dittmar, T A1 - Meyer, JL A1 - Huber, JA A1 - Glazer, BT A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2012 ER - TY - JOUR T1 - Anaerobic methane oxidation in metalliferous hydrothermal sediments: influence on carbon flux and decoupling from sulfate reduction JF - Environmental Microbiology Y1 - 2012 A1 - Wankel, S. D. A1 - Adams, M. M. A1 - Johnston, D. T. A1 - Hansel, C. M. A1 - Joye, S. B. A1 - Girguis, P. R. KW - de-fuca ridge KW - guaymas basin KW - gulf-of-mexico KW - in-vitro KW - marine-sediments KW - middle valley KW - molecular-weight hydrocarbons KW - organic-matter KW - reducing bacteria KW - skagerrak denmark AB -

The anaerobic oxidation of methane (AOM) is a globally significant sink that regulates methane flux from sediments into the oceans and atmosphere. Here we examine mesophilic to thermophilic AOM in hydrothermal sediments recovered from the Middle Valley vent field, on the Juan de Fuca Ridge. Using continuous-flow sediment bioreactors and batch incubations, we characterized (i) the degree to which AOM contributes to net dissolved inorganic carbon flux, (ii) AOM and sulfate reduction (SR) rates as a function of temperature and (iii) the distribution and density of known anaerobic methanotrophs (ANMEs). In sediment bioreactors, inorganic carbon stable isotope mass balances results indicated that AOM accounted for between 16% and 86% of the inorganic carbon produced, underscoring the role of AOM in governing inorganic carbon flux from these sediments. At 90 degrees C, AOM occurred in the absence of SR, demonstrating a striking decoupling of AOM from SR. An abundance of Fe(III)-bearing minerals resembling mixed valent Fe oxides, such as green rust, suggests the potential for a coupling of AOM to Fe(III) reduction in these metalliferous sediments. While SR bacteria were only observed in cooler temperature sediments, ANMEs allied to ANME-1 ribotypes, including a putative ANME-1c group, were found across all temperature regimes and represented a substantial proportion of the archaeal community. In concert, these results extend and reshape our understanding of the nature of high temperature methane biogeochemistry, providing insight into the physiology and ecology of thermophilic anaerobic methanotrophy and suggesting that AOM may play a central role in regulating biological dissolved inorganic carbon fluxes to the deep ocean from the organic-poor, metalliferous sediments of the global mid-ocean ridge hydrothermal vent system.

VL - 14 SN - 1462-2912 N1 -

Sp. Iss. SI015KYTimes Cited:10Cited References Count:71

JO - Environ MicrobiolEnviron Microbiol ER - TY - JOUR T1 - Duty Cycling Influences Current Generation in Multi-Anode Environmental Microbial Fuel Cells. JF - Environmental Science & Technology Y1 - 2012 A1 - Gardel, E. J. A1 - Nielsen, M. E. A1 - Grisdela, P. T. A1 - Girguis, P. R. KW - biofuel cells KW - communities KW - elemental sulfur KW - extracellular electron-transfer KW - geobacter-sulfurreducens KW - harvesting electricity KW - power-generation KW - sediment KW - sulfate-reducing bacteria KW - waste-water treatment AB -

Improving microbial fuel cell (MFC) performance continues to be the subject of research, yet the role of operating conditions, specifically duty cycling, on MFC performance has been modestly addressed. We present a series of studies in which we use a 15-anode environmental MFC to explore how duty cycling (variations in the time an anode is connected) influences cumulative charge, current, and microbial composition. The data reveal particular switching intervals that result in the greatest time-normalized current. When disconnection times are sufficiently short, there is a striking decrease in current due to an increase in the overall electrode reaction resistance. This was observed over a number of whole cell potentials. Based on these results, we posit that replenishment of depleted electron donors within the biofilm and surrounding diffusion layer is necessary for maximum charge transfer, and that proton flux may be not limiting in the highly buffered aqueous phases that are common among environmental MFCs. Surprisingly, microbial diversity analyses found no discernible difference in gross community composition among duty cycling treatments, suggesting that duty cycling itself has little or no effect. Such duty cycling experiments are valuable in determining which factors govern performance of bioelectrochemical systems and might also be used to optimize field-deployed systems.

VL - 46 SN - 0013-936X N1 -

933GOTimes Cited:9Cited References Count:66

JO - Environ Sci TechnolEnviron Sci Technol ER - TY - JOUR T1 - Evidence for the role of endosymbionts in regional-scale habitat partitioning by hydrothermal vent symbioses. JF - Proceedings of the National Academy of Sciences of the United States of America Y1 - 2012 A1 - Beinart, R. A. A1 - Sanders, J. G. A1 - Faure, B. A1 - Sylva, S. P. A1 - Lee, R. W. A1 - Becker, E. L. A1 - Gartman, A. A1 - Luther, G. W. A1 - Seewald, J. S. A1 - Fisher, C. R. A1 - Girguis, P. R. KW - chemoautotrophic symbionts KW - chemoautotrophy KW - Community structure KW - east pacific rise KW - endosymbiosis KW - epsilon-proteobacteria KW - host-plant use KW - lau-spreading-center KW - mussel bathymodiolus-thermophilus KW - riftia-pachyptila jones KW - rose garden vent KW - symbiosis KW - tube worm AB -

Deep-sea hydrothermal vents are populated by dense communities of animals that form symbiotic associations with chemolithoautotrophic bacteria. To date, our understanding of which factors govern the distribution of host/symbiont associations (or holobionts) in nature is limited, although host physiology often is invoked. In general, the role that symbionts play in habitat utilization by vent holobionts has not been thoroughly addressed. Here we present evidence for symbiont-influenced, regional-scale niche partitioning among symbiotic gastropods (genus Alviniconcha) in the Lau Basin. We extensively surveyed Alviniconcha holobionts from four vent fields using quantitative molecular approaches, coupled to characterization of high-temperature and diffuse vent-fluid composition using gastight samplers and in situ electrochemical analyses, respectively. Phylogenetic analyses exposed cryptic host and symbiont diversity, revealing three distinct host types and three different symbiont phylotypes (one epsilon-proteobacteria and two gamma-proteobacteria) that formed specific associations with one another. Strikingly, we observed that holobionts with epsilon-proteobacterial symbionts were dominant at the northern fields, whereas holobionts with gamma-proteobacterial symbionts were dominant in the southern fields. This pattern of distribution corresponds to differences in the vent geochemistry that result from deep subsurface geological and geothermal processes. We posit that the symbionts, likely through differences in chemolithoautotrophic metabolism, influence niche utilization among these holobionts. The data presented here represent evidence linking symbiont type to habitat partitioning among the chemosynthetic symbioses at hydrothermal vents and illustrate the coupling between subsurface geothermal processes and niche availability.

VL - 109 SN - 0027-8424 N1 -

049PZTimes Cited:11Cited References Count:88

JO - P Natl Acad Sci USAP Natl Acad Sci USA ER - TY - JOUR T1 - Exploring the limit of metazoan thermal tolerance via comparative proteomics: thermally induced changes in protein abundance by two hydrothermal vent polychaetes JF - Proceedings of the Royal Society B-Biological Sciences Y1 - 2012 A1 - Dilly, G. F. A1 - Young, C. R. A1 - Lane, W. S. A1 - Pangilinan, J. A1 - Girguis, P. R. KW - Animals KW - climate-change KW - fishes KW - Heat-Shock Proteins KW - hydrothermal vents KW - mitochondria KW - Molecular Chaperones KW - oxidative stress KW - oxygen limitation KW - paralvinella KW - proteomics KW - redox KW - temperature-dependent biogeography KW - thermotolerance AB -

Temperatures around hydrothermal vents are highly variable, ranging from near freezing up to 300 degrees C. Nevertheless, animals thrive around vents, some of which live near the known limits of animal thermotolerance. Paralvinella sulfincola, an extremely thermotolerant vent polychaete, and Paralvinella palmiformis, a cooler-adapted congener, are found along the Juan de Fuca Ridge in the northwestern Pacific. We conducted shipboard high-pressure thermotolerance experiments on both species to characterize the physiological adaptations underlying P. sulfincola's pronounced thermotolerance. Quantitative proteomics, expressed sequence tag (EST) libraries and glutathione assays revealed that P. sulfincola (i) exhibited an upregulation in the synthesis and recycling of glutathione with increasing temperature, (ii) downregulated nicotinamide adenine dinucleotide (NADH) and succinate dehydrogenases (key enzymes in oxidative phosphorylation) with increasing temperature, and (iii) maintained elevated levels of heat shock proteins (HSPs) across all treatments. In contrast, P. palmiformis exhibited more typical responses to increasing temperatures (e. g. increasing HSPs at higher temperatures). These data reveal differences in how a mesotolerant and extremely thermotolerant eukaryote respond to thermal stress, and suggest that P. sulfincola's capacity to mitigate oxidative stress via increased synthesis of antioxidants and decreased flux through the mitochondrial electron transport chain enable pronounced thermotolerance. Ultimately, oxidative stress may be the key factor in limiting all metazoan thermotolerance.

VL - 279 SN - 0962-8452 N1 -

973DQTimes Cited:8Cited References Count:53

JO - P Roy Soc B-Biol SciP Roy Soc B-Biol Sci ER - TY - JOUR T1 - Expression and Putative Function of Innate Immunity Genes under in situ Conditions in the Symbiotic Hydrothermal Vent Tubeworm Ridgeia piscesae JF - Plos One Y1 - 2012 A1 - Nyholm, S. V. A1 - Song, P. F. A1 - Dang, J. N. A1 - Bunce, C. A1 - Girguis, P. R. KW - bacterial symbiosis KW - carbonic-anhydrase KW - chemoautotrophic symbionts KW - host KW - localization KW - metabolite uptake KW - recognition KW - sulfide binding KW - toll-like receptors KW - worm riftia-pachyptila AB -

The relationships between hydrothermal vent tubeworms and sulfide-oxidizing bacteria have served as model associations for understanding chemoautotrophy and endosymbiosis. Numerous studies have focused on the physiological and biochemical adaptations that enable these symbioses to sustain some of the highest recorded carbon fixation rates ever measured. However, far fewer studies have explored the molecular mechanisms underlying the regulation of host and symbiont interactions, specifically those mediated by the innate immune system of the host. To that end, we conducted a series of studies where we maintained the tubeworm, Ridgeia piscesae, in high-pressure aquaria and examined global and quantitative changes in gene expression via high-throughput transcriptomics and quantitative real-time PCR (qPCR). We analyzed over 32,000 full-length expressed sequence tags as well as 26 Mb of transcript sequences from the trophosome (the organ that houses the endosymbiotic bacteria) and the plume (the gas exchange organ in contact with the free-living microbial community). R. piscesae maintained under conditions that promote chemoautotrophy expressed a number of putative cell signaling and innate immunity genes, including pattern recognition receptors (PRRs), often associated with recognizing microbe-associated molecular patterns (MAMPs). Eighteen genes involved with innate immunity, cell signaling, cell stress and metabolite exchange were further analyzed using qPCR. PRRs, including five peptidoglycan recognition proteins and a Toll-like receptor, were expressed significantly higher in the trophosome compared to the plume. Although PRRs are often associated with mediating host responses to infection by pathogens, the differences in expression between the plume and trophosome also implicate similar mechanisms of microbial recognition in interactions between the host and symbiont. We posit that regulation of this association involves a molecular "dialogue'' between the partners that includes interactions between the host's innate immune system and the symbiont.

VL - 7 SN - 1932-6203 IS - 6 N1 -

959TUTimes Cited:3Cited References Count:86

JO - Plos OnePlos One ER - TY - JOUR T1 - In situ chemistry and microbial community compositions in five deep-sea hydrothermal fluid samples from Irina II in the Logatchev field JF - Environmental Microbiology Y1 - 2012 A1 - Perner, M. A1 - Gonnella, G. A1 - Hourdez, S. A1 - Bohnke, S. A1 - Kurtz, S. A1 - Girguis, P. KW - *Biodiversity KW - Hydrogen-Ion Concentration KW - Hydrothermal Vents/*chemistry/*microbiology KW - Magnesium/analysis KW - Oxygen/analysis KW - Proteobacteria/genetics/isolation & purification KW - RNA, Ribosomal, 16S/genetics KW - Seawater/*chemistry/*microbiology KW - Temperature KW - Time Factors AB -

We present data on the co-registered geochemistry (in situ mass spectrometry) and microbiology (pyrosequencing of 16S rRNA genes; V1, V2, V3 regions) in five fluid samples from Irina II in the Logatchev hydrothermal field. Two samples were collected over 24 min from the same spot and further three samples were from spatially distinct locations (20 cm, 3 m and the overlaying plume). Four low-temperature hydrothermal fluids from the Irina II are composed of the same core bacterial community, namely specific Gammaproteobacteria and Epsilonproteobacteria, which, however, differs in the relative abundance. The microbial composition of the fifth sample (plume) is considerably different. Although a significant correlation between sulfide enrichment and proportions of Sulfurovum (Epsilonproteobacteria) was found, no other significant linkages between abiotic factors, i.e. temperature, hydrogen, methane, sulfide and oxygen, and bacterial lineages were evident. Intriguingly, bacterial community compositions of some time series samples from the same spot were significantly more similar to a sample collected 20 cm away than to each other. Although this finding is based on three single samples only, it provides first hints that single hydrothermal fluid samples collected on a small spatial scale may also reflect unrecognized temporal variability. However, further studies are required to support this hypothesis.

VL - 15 SN - 1462-2920 (Electronic)1462-2912 (Linking) N1 -

Perner, MirjamGonnella, GiorgioHourdez, StephaneBohnke, StefanieKurtz, StefanGirguis, PeterengResearch Support, Non-U.S. Gov'tEngland2012/11/23 06:00Environ Microbiol. 2013 May;15(5):1551-60. doi: 10.1111/1462-2920.12038. Epub 2012 Nov 22.

JO - Environ MicrobiolEnviron Microbiol ER - TY - JOUR T1 - Links from Mantle to Microbe at the Lau Integrated Study Site: Insights from a Back-Arc Spreading Center JF - Oceanography Y1 - 2012 A1 - Tivey, M. K. A1 - Becker, E. A1 - Beinart, R. A1 - Fisher, C. R. A1 - Girguis, P. R. A1 - Langmuir, C. H. A1 - Michael, P. J. A1 - Reysenbach, A. L. KW - basin KW - crustal structure KW - de-fuca ridge KW - east pacific rise KW - endeavor segment KW - flow KW - growth KW - rose garden vent KW - sea hydrothermal vents KW - Speciation AB -

The Lau Integrated Study Site (ISS) has provided unique opportunities for study of ridge processes because of its back-arc setting in the southwestern Pacific. Its location allows study of a biogeographical province distinct from those of eastern Pacific and mid-Atlantic ridges, and crustal compositions along the ridge lie outside the range of mid-ocean ridge crustal compositions. The Lau ISS is located above a subduction zone, at an oblique angle. The underlying mantle receives water and other elements derived from the downgoing lithospheric slab, with an increase in slab influence from north to south. Water lowers the mantle melting temperature and leads to greater melt production where the water flux is greater, and to distinctive regional-scale gradients along the ridge. There are deeper faulted axial valleys with basaltic volcanism in the north and inflated axial highs with andesites in the south. Differences in igneous rock composition and release of magmatic volatiles affect compositions of vent fluids and deposits. Differences in vent fluid compositions and small-scale diffuse-flow regimes correlate with regional-scale patterns in microbial and megafaunal distributions. The interdisciplinary research effort at the Lau ISS has successfully identified linkages between subsurface processes and deep-sea biological communities, from mantle to microbe to megafauna.

VL - 25 SN - 1042-8275 IS - 1 N1 -

Sp. Iss. SI903DCTimes Cited:8Cited References Count:70

JO - OceanographyOceanography ER - TY - JOUR T1 - On the Potential for Bioenergy and Biofuels from Hydrothermal Vent Microbes. JF - Oceanography Y1 - 2012 A1 - Girguis, P. R. A1 - Holden, J. F. KW - energy KW - fuel-cells VL - 25 SN - 1042-8275 IS - 1 N1 -

Sp. Iss. SI903DCTimes Cited:2Cited References Count:17

JO - OceanographyOceanography ER - TY - JOUR T1 - Benthic microbial fuel cell as direct power source for an acoustic modem and seawater oxygen/temperature sensor system JF - Environmental science & technology Y1 - 2011 A1 - Gong, Yanming A1 - Radachowsky, Sage E A1 - Michael Wolf A1 - Nielsen, Mark E A1 - Girguis, Peter R. A1 - Reimers, Clare E PB - American Chemical Society VL - 45 IS - 11 ER - TY - CONF T1 - Characterizing Electrosynthetic Microbial Production of Acetate T2 - ECS Meeting Abstracts Y1 - 2011 A1 - Nielsen, Mark E A1 - Peter Girguis JF - ECS Meeting Abstracts PB - IOP Publishing IS - 4 ER - TY - JOUR T1 - Influence of subsurface biosphere on geochemical fluxes from diffuse hydrothermal fluids JF - Nature Geoscience Y1 - 2011 A1 - Wankel, Scott D A1 - Germanovich, Leonid N A1 - Lilley, Marvin D A1 - Genc, Gence A1 - DiPerna, Christopher J A1 - Bradley, Alexander S A1 - Olson, Eric J A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 4 IS - 7 ER - TY - JOUR T1 - Linking hydrothermal geochemistry to organismal physiology: physiological versatility in Riftia pachyptila from sedimented and basalt-hosted vents JF - PLoS One Y1 - 2011 A1 - Robidart, Julie C. A1 - Roque, Annelys A1 - Song, Pengfei A1 - Girguis, Peter R. PB - Public Library of Science VL - 6 IS - 7 ER - TY - CHAP T1 - Measuring isotope fractionation by autotrophic microorganisms and enzymes T2 - Methods in Enzymology Y1 - 2011 A1 - Scott, Kathleen M A1 - Fox, Gordon A1 - Girguis, Peter R. JF - Methods in Enzymology PB - Academic Press VL - 494 ER - TY - JOUR T1 - Metabolic and practical considerations on microbial electrosynthesis JF - Current opinion in biotechnology Y1 - 2011 A1 - Rabaey, Korneel A1 - Peter Girguis A1 - Nielsen, Lars K PB - Elsevier Current Trends VL - 22 IS - 3 ER - TY - JOUR T1 - The metabolic demands of endosymbiotic chemoautotrophic metabolism on host physiological capacities JF - Journal of Experimental Biology Y1 - 2011 A1 - Childress, J. J. A1 - Girguis, Peter R. PB - Company of Biologists VL - 214 IS - 2 ER - TY - ABST T1 - Methane-powered microbial fuel cells Y1 - 2011 A1 - Peter Girguis A1 - Reimers, Clare E N1 - US Patent App. 12/994,598 ER - TY - JOUR T1 - Thermodynamics and kinetics of sulfide oxidation by oxygen: a look at inorganically controlled reactions and biologically mediated processes in the environment JF - Frontiers in microbiology Y1 - 2011 A1 - Luther, George W A1 - Findlay, Alyssa J A1 - MacDonald, Daniel J A1 - Owings, Shannon M A1 - Hanson, Thomas E A1 - Beinart, Roxanne A A1 - Girguis, Peter R. PB - Frontiers VL - 2 ER - TY - JOUR T1 - Benthic microbial fuel cell as direct power source for an acoustic modem and seawater oxygen/temperature sensor system JF - Environmental science & technology Y1 - 2011 A1 - Gong, Yanming A1 - Radachowsky, Sage E A1 - Michael Wolf A1 - Nielsen, Mark E A1 - Girguis, Peter R. A1 - Reimers, Clare E PB - American Chemical Society VL - 45 IS - 11 ER - TY - CONF T1 - Characterizing Electrosynthetic Microbial Production of Acetate T2 - ECS Meeting Abstracts Y1 - 2011 A1 - Nielsen, Mark E A1 - Peter Girguis JF - ECS Meeting Abstracts PB - IOP Publishing IS - 4 ER - TY - JOUR T1 - Hydrogen is an energy source for hydrothermal vent symbioses JF - Nature Y1 - 2011 A1 - Petersen, Jillian M A1 - Zielinski, Frank U A1 - Pape, Thomas A1 - Seifert, Richard A1 - Moraru, Cristina A1 - Amann, Rudolf A1 - Hourdez, Stephane A1 - Girguis, Peter R. A1 - Wankel, Scott D A1 - Barbe, Valerie A1 - others PB - Nature Publishing Group VL - 476 IS - 7359 ER - TY - CONF T1 - Increased carbon uptake in marine sediment enabled by naturally occurring electrical conductors T2 - AGU Fall Meeting Abstracts Y1 - 2011 A1 - Nielsen, M. E. A1 - Cahoon, DP A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2011 ER - TY - CONF T1 - Influence Of Subsurface Biosphere On Geochemical Fluxes From Diffuse Hydrothermal Fluids: Direct Measurement Of Subsurface Hydrogen Oxidation T2 - AGU Fall Meeting Abstracts Y1 - 2011 A1 - Wankel, S. D. A1 - Germanovich, L. N. A1 - Lilley, M. D. A1 - Genc, G. A1 - DiPerna, C. J. A1 - Bradley, A. S. A1 - Olson, E. J. A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2011 ER - TY - JOUR T1 - Influence of subsurface biosphere on geochemical fluxes from diffuse hydrothermal fluids JF - Nature Geoscience Y1 - 2011 A1 - Wankel, Scott D A1 - Germanovich, Leonid N A1 - Lilley, Marvin D A1 - Genc, Gence A1 - DiPerna, Christopher J A1 - Bradley, Alexander S A1 - Olson, Eric J A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 4 IS - 7 ER - TY - JOUR T1 - Linking hydrothermal geochemistry to organismal physiology: physiological versatility in Riftia pachyptila from sedimented and basalt-hosted vents JF - PLoS One Y1 - 2011 A1 - Robidart, Julie C. A1 - Roque, Annelys A1 - Song, Pengfei A1 - Girguis, Peter R. PB - Public Library of Science VL - 6 IS - 7 ER - TY - CHAP T1 - Measuring isotope fractionation by autotrophic microorganisms and enzymes T2 - Methods in Enzymology Y1 - 2011 A1 - Scott, Kathleen M A1 - Fox, Gordon A1 - Girguis, Peter R. JF - Methods in Enzymology PB - Academic Press VL - 494 ER - TY - JOUR T1 - Metabolic and practical considerations on microbial electrosynthesis JF - Current opinion in biotechnology Y1 - 2011 A1 - Rabaey, Korneel A1 - Peter Girguis A1 - Nielsen, Lars K PB - Elsevier Current Trends VL - 22 IS - 3 ER - TY - JOUR T1 - The metabolic demands of endosymbiotic chemoautotrophic metabolism on host physiological capacities JF - Journal of Experimental Biology Y1 - 2011 A1 - Childress, J. J. A1 - Girguis, Peter R. PB - Company of Biologists VL - 214 IS - 2 ER - TY - ABST T1 - Methane-powered microbial fuel cells Y1 - 2011 A1 - Peter Girguis A1 - Reimers, Clare E N1 - US Patent App. 12/994,598 ER - TY - CONF T1 - Microbiology of Low Temperature Seafloor Deposits Along a Geochemical Gradient in Lau Basin T2 - AGU Fall Meeting Abstracts Y1 - 2011 A1 - Sia, T. Y. A1 - Haddad, A A1 - Briscoe, L. J. A1 - Girguis, P. R. A1 - Edwards, K. J. A1 - others JF - AGU Fall Meeting Abstracts VL - 2011 ER - TY - CONF T1 - Nature and extent of electrogenic microbial communities recovered from Juan de Fuca hydrothermal sulfides T2 - AGU Fall Meeting Abstracts Y1 - 2011 A1 - Girguis, P. R. A1 - Nielsen, M. E. JF - AGU Fall Meeting Abstracts VL - 2011 ER - TY - CONF T1 - Physico-chemical gradients within the hydrothermal chimney Roane define sharp boundaries for microbial community ecology T2 - AGU Fall Meeting Abstracts Y1 - 2011 A1 - Frank, K. L. A1 - Kelley, DS A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2011 ER - TY - JOUR T1 - Sulfide oxidation across diffuse flow zones of hydrothermal vents JF - Aquatic Geochemistry Y1 - 2011 A1 - Gartman, Amy A1 - Yücel, Mustafa A1 - Madison, Andrew S A1 - Chu, David W A1 - Ma, Shufen A1 - Janzen, Christopher P A1 - Becker, Erin L A1 - Beinart, Roxanne A A1 - Girguis, Peter R. A1 - Luther, George W PB - Springer Netherlands VL - 17 IS - 4 ER - TY - JOUR T1 - Thermodynamics and kinetics of sulfide oxidation by oxygen: a look at inorganically controlled reactions and biologically mediated processes in the environment JF - Frontiers in microbiology Y1 - 2011 A1 - Luther, George W A1 - Findlay, Alyssa J A1 - MacDonald, Daniel J A1 - Owings, Shannon M A1 - Hanson, Thomas E A1 - Beinart, Roxanne A A1 - Girguis, Peter R. PB - Frontiers VL - 2 ER - TY - JOUR T1 - Benthic Microbial Fuel Cell as Direct Power Source for an Acoustic Modem and Seawater Oxygen/Temperature Sensor System. JF - Environmental Science & Technology Y1 - 2011 A1 - Gong, Y. M. A1 - Radachowsky, S. E. A1 - Wolf, M. A1 - Nielsen, M. E. A1 - Girguis, P. R. A1 - Reimers, C. E. KW - anode KW - electricity production KW - energy KW - generation KW - in-situ KW - microorganisms KW - sea-floor AB -

Supported by the natural potential difference between anoxic sediment and oxic seawater, benthic microbial fuel cells (BMFCs) promise to be ideal power sources for certain low-power marine sensors and communication devices. In this study a chambered BMFC with a 0.25 m(2) footprint was used to power an acoustic modem interfaced with an oceanographic sensor that measures dissolved oxygen and temperature. The experiment was conducted in Yaquina Bay, Oregon over 50 days. Several improvements were made in the BMFC design and power management system based on lessons learned from earlier prototypes. The energy was harvested by a dynamic gain charge pump circuit that maintains a desired point on the BMFC's power curve and stores the energy in a 200 F supercapacitor. The system also used an ultralow power microcontroller and quartz clock to read the oxygen/temperature sensor hourly, store data with a time stamp, and perform daily polarizations. Data records were transmitted to the surface by the acoustic modem every 1-5 days after receiving an acoustic prompt from a surface hydrophone. After jump-starting energy production with supplemental macroalgae placed in the BMFC's anode chamber, the average power density of the BMFC adjusted to 44 mW/m(2) of seafloor area which is better than past demonstrations at this site. The highest power density was 158 mW/m(2), and the useful energy produced and stored was >= 1.7 times the energy required to operate the system.

VL - 45 SN - 0013-936X N1 -

771ADTimes Cited:18Cited References Count:36

JO - Environ Sci TechnolEnviron Sci Technol ER - TY - JOUR T1 - Hydrogen is an energy source for hydrothermal vent symbioses. JF - Nature Y1 - 2011 A1 - Petersen, J. M. A1 - Zielinski, F. U. A1 - Pape, T. A1 - Seifert, R. A1 - Moraru, C. A1 - Amann, R. A1 - Hourdez, S. A1 - Girguis, P. R. A1 - Wankel, S. D. A1 - Barbe, V. A1 - Pelletier, E. A1 - Fink, D. A1 - Borowski, C. A1 - Bach, W. A1 - Dubilier, N. KW - Bacteria KW - dual symbiosis KW - fluids KW - h-2 KW - membrane-bound hydrogenase KW - mid-atlantic ridge KW - molecular-hydrogen KW - mussels KW - riftia-pachyptila jones KW - tube worm AB -

The discovery of deep-sea hydrothermal vents in 1977 revolutionized our understanding of the energy sources that fuel primary productivity on Earth. Hydrothermal vent ecosystems are dominated by animals that live in symbiosis with chemosynthetic bacteria. So far, only two energy sources have been shown to power chemosynthetic symbioses: reduced sulphur compounds and methane. Using metagenome sequencing, single-gene fluorescence in situ hybridization, immunohistochemistry, shipboard incubations and in situ mass spectrometry, we show here that the symbionts of the hydrothermal vent mussel Bathymodiolus from the Mid-Atlantic Ridge use hydrogen to power primary production. In addition, we show that the symbionts of Bathymodiolus mussels from Pacific vents have hupL, the key gene for hydrogen oxidation. Furthermore, the symbionts of other vent animals such as the tubeworm Riftia pachyptila and the shrimp Rimicaris exoculata also have hupL. We propose that the ability to use hydrogen as an energy source is widespread in hydrothermal vent symbioses, particularly at sites where hydrogen is abundant.

VL - 476 SN - 0028-0836 N1 -

805MUTimes Cited:47Cited References Count:48

JO - NatureNature ER - TY - JOUR T1 - Influence of subsurface biosphere on geochemical fluxes from diffuse hydrothermal fluids JF - Nature Geoscience Y1 - 2011 A1 - Wankel, S. D. A1 - Germanovich, L. N. A1 - Lilley, M. D. A1 - Genc, G. A1 - DiPerna, C. J. A1 - Bradley, A. S. A1 - Olson, E. J. A1 - Girguis, P. R. KW - de-fuca-ridge KW - east pacific rise KW - heat KW - isotopic evidence KW - main endeavor field KW - mass-spectrometry KW - riftia-pachyptila KW - sea-floor KW - temporal variability KW - vent fluids AB -

Hydrothermal vents along mid-ocean systems host unique, highly productive biological communities, based on microbial chemoautotrophy, that thrive on the sulphur, metals, nitrogen and carbon emitted from the vents into the deep ocean. Geochemical studies of vents have centred on analyses of high-temperature, focused hydrothermal vents, which exhibit very high flow rates and are generally considered too hot for microbial life. Geochemical fluxes and metabolic activity associated with habitable, lower temperature diffuse fluids remain poorly constrained. As a result, little is known about the extent to which microbial communities, particularly in the subsurface, influence geochemical flux from more diffuse flows. Here, we estimate the net flux of methane, carbon dioxide and hydrogen from diffuse and focused hydrothermal vents along the Juan de Fuca ridge, using an in situ mass spectrometer and flowmeter. We show that geochemical flux from diffuse vents can equal or exceed that emanating from hot, focused vents. Notably, hydrogen concentrations in fluids emerging from diffuse vents are 50% to 80% lower than predicted. We attribute the loss of hydrogen in diffuse vent fluids to microbial consumption in the subsurface, and suggest that subsurface microbial communities can significantly influence hydrothermal geochemical fluxes to the deep ocean.

VL - 4 SN - 1752-0894 N1 -

785PVTimes Cited:28Cited References Count:50

JO - Nat GeosciNat Geosci ER - TY - JOUR T1 - Linking Hydrothermal Geochemistry to Organismal Physiology: Physiological Versatility in Riftia pachyptila from Sedimented and Basalt-hosted Vents JF - Plos One Y1 - 2011 A1 - Robidart, Julie C. A1 - Roque, Annelys A1 - Song, Pengfei A1 - Girguis, Peter R. KW - Abiotic factors KW - Annelids KW - Associations KW - Evolution KW - Habitat KW - Invertebrates KW - Marine habitat KW - Marine zones KW - Pacific Ocean KW - Whole animal physiology AB -

Much of what is known regarding Riftia pachyptila physiology is based on the wealth of studies of tubeworms living at diffuse flows along the fast-spreading, basalt-hosted East Pacific Rise (EPR). These studies have collectively suggested that Riftia pachyptila and its chemoautotrophic symbionts are physiologically specialized, highly productive associations relying on hydrogen sulfide and oxygen to generate energy for carbon fixation, and the symbiont's nitrate reduction to ammonia for energy and biosynthesis. However, Riftia also flourish in sediment-hosted vents, which are markedly different in geochemistry than basalt-hosted systems. Here we present data from shipboard physiological studies and global quantitative proteomic analyses of Riftia pachyptila trophosome tissue recovered from tubeworms residing in the EPR and the Guaymas basin, a sedimented, hydrothermal vent field. We observed marked differences in symbiont nitrogen metabolism in both the respirometric and proteomic data. The proteomic data further suggest that Riftia associations in Guaymas may utilize different sulfur compounds for energy generation, may have an increased capacity for energy storage, and may play a role in degrading exogenous organic carbon. Together these data reveal that Riftia symbionts are far more physiologically plastic than previously considered, and that -contrary to previous assertions- Riftia do assimilate reduced nitrogen in some habitats. These observations raise new hypotheses regarding adaptations to the geochemical diversity of habitats occupied by Riftia, and the degree to which the environment influences symbiont physiology and evolution.

VL - 6 IS - 7 N1 -

pgirguis@oeb.harvard.edu

JO - Plos One ER - TY - JOUR T1 - Measuring Isotope Fractionation by Autotrophic Microorganisms and Enzymes JF - Methods in Enzymology: Methods in Methane Metabolism, Pt A Y1 - 2011 A1 - Scott, K. M. A1 - Fox, G. A1 - Girguis, P. R. KW - atmospheric methane KW - carbon-dioxide KW - continuous-flow bioreactor KW - form ia-rubisco KW - gas-chromatography KW - high-precision KW - inlet mass-spectrometry KW - ribulose bisphosphate KW - tricarboxylic-acid cycle KW - tubeworm riftia-pachyptila AB -

Physical, chemical, and biological processes commonly discriminate among stable isotopes. Therefore, the stable isotope compositions of biomass, growth substrates, and products often carry the isotopic fingerprints of the processes that shape them. Therefore, measuring isotope fractionation by enzymes and cultures of autotrophic microorganisms can provide insights at many levels, from metabolism to ecosystem function. Discussed here are considerations relevant to measuring isotope discrimination by enzymes as well at, intact cells, with an emphasis on stable one-carbon isotopes and autotrophic microorganisms.

VL - 494 SN - 0076-6879 N1 -

Bui30Times Cited:1Cited References Count:78Methods in Enzymology

JO - Method EnzymolMethod Enzymol ER - TY - JOUR T1 - Metabolic and practical considerations on microbial electrosynthesis JF - Current Opinion in Biotechnology Y1 - 2011 A1 - Rabaey, K. A1 - Girguis, P. A1 - Nielsen, L. K. KW - *Electricity KW - *Microbiological Phenomena KW - Biofuels/economics KW - Biosynthetic Pathways KW - Carbon/metabolism KW - Cost-Benefit Analysis KW - Dexfenfluramine KW - Electrons KW - Fermentation KW - Glucose/metabolism AB -

The production of biofuels and biochemicals is highly electron intensive. To divert fermentative and respiratory pathways to the product of interest, additional electrons (i.e. reducing power) are often needed. Meanwhile, the past decade has seen the breakthrough of sustainable electricity sources such as solar and wind. Microbial electrosynthesis (MES) is at the nexus of both, as it uses electrical energy as source of reducing power for microorganisms. This review addresses the key opportunities and challenges for MES. While exciting as a concept, MES needs to overcome many biological, electrochemical, logistical and economic challenges. Particularly the latter is critical, as on a 'per electron basis' MES does not yet appear to deliver a substantial benefit relative to existing approaches.

VL - 22 SN - 1879-0429 (Electronic)0958-1669 (Linking) N1 -

Rabaey, KorneelGirguis, PeterNielsen, Lars KengResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.ReviewEngland2011/03/01 06:00Curr Opin Biotechnol. 2011 Jun;22(3):371-7. doi: 10.1016/j.copbio.2011.01.010. Epub 2011 Feb 23.

JO - Curr Opin Biotech ER - TY - JOUR T1 - The metabolic demands of endosymbiotic chemoautotrophic metabolism on host physiological capacities JF - Journal of Experimental Biology Y1 - 2011 A1 - Childress, J. J. A1 - Girguis, P. R. KW - Annelids KW - Associations KW - Biochemistry KW - Evolution KW - Invertebrates KW - Molluscs AB -

While chemoautotrophic endosymbioses of hydrothermal vents and other reducing environments have been well studied, little attention has been paid to the magnitude of the metabolic demands placed upon the host by symbiont metabolism and the adaptations necessary to meet such demands. Here we make the first attempt at such an evaluation, and show that moderate to high rates of chemoautotrophic or methanotrophic metabolism impose oxygen uptake and proton equivalent elimination demands upon the hosts that are much higher than is typical for the non-symbiotic annelid, bivalve and gastropod lineages to which they are related. The properties of the hosts are described and compared to determine which properties are associated with and predictive of the highest rates. We suggest that the high oxygen demand of these symbionts is perhaps the most limiting flux for the symbioses. Among the consequences of such demands has been the widespread presence of circulating and/or tissue hemoglobins in these symbioses that are necessary to support high metabolic rates in thioautotrophic endosymbioses. We also compare photoautotrophic with chemoautotrophic and methanotrophic endosymbioses to evaluate the differences and similarities in physiologies. These analyses suggest that the high demand for oxygen by chemoautotrophic and methanotrophic symbionts is likely a major factor precluding their endosymbiosis with cnidarians.

VL - 214 N1 -

childres@lifesci.ucsb.edu

ER - TY - JOUR T1 - Sulfide Oxidation across Diffuse Flow Zones of Hydrothermal Vents JF - Aquatic Geochemistry Y1 - 2011 A1 - Gartman, A. A1 - Yucel, M. A1 - Madison, A. S. A1 - Chu, D. W. A1 - Ma, S. F. A1 - Janzen, C. P. A1 - Becker, E. L. A1 - Beinart, R. A. A1 - Girguis, P. R. A1 - Luther, G. W. KW - aqueous-solution KW - diffuse flow KW - east pacific rise KW - elemental sulfur KW - hydrogen-sulfide KW - hydrothermal vents KW - in situ chemistry KW - in-situ KW - Kinetics KW - lau basin KW - lau spreading center KW - riftia-pachyptila KW - species distributions KW - sulfide oxidation KW - sulfur speciation KW - voltammetric microelectrode AB -

The sulfide (H(2)S/HS(-)) that is emitted from hydrothermal vents begins to oxidize abiotically with oxygen upon contact with ambient bottom water, but the reaction kinetics are slow. Here, using in situ voltammetry, we report detection of the intermediate sulfur oxidation products polysulfides [S(x)(2-)] and thiosulfate [S(2)O(3)(2-)], along with contextual data on sulfide, oxygen, and temperature. At Lau Basin in 2006, thiosulfate was identified in less than one percent of approximately 10,500 scans and no polysulfides were detected. Only five percent of 11,000 voltammetric scans taken at four vent sites at Lau Basin in May 2009 show either thiosulfate or polysulfides. These in situ data indicate that abiotic sulfide oxidation does not readily occur as H(2)S contacts oxic bottom waters. Calculated abiotic potential sulfide oxidation rates are < 10(-3) mu M/min and are consistent with slow oxidation and the observed lack of sulfur oxidation intermediates. It is known that the thermodynamics for the first electron transfer step for sulfide and oxygen during sulfide oxidation in these systems are unfavorable, and that the kinetics for two electron transfers are not rapid. Here, we suggest that different metal catalyzed and/or biotic reaction pathways can readily produce sulfur oxidation intermediates. Via shipboard high-pressure incubation experiments, we show that snails with chemosynthetic endosymbionts do release polysulfides and may be responsible for our field observations of polysulfides.

VL - 17 SN - 1380-6165 N1 -

Sp. Iss. SI814MYTimes Cited:12Cited References Count:57

JO - Aquat GeochemAquat Geochem ER - TY - JOUR T1 - Thermodynamics and kinetics of sulfide oxidation by oxygen: a look at inorganically controlled reactions and biologically mediated processes in the environment JF - Frontiers in Microbiology Y1 - 2011 A1 - Luther, G. W. A1 - Findlay, A. J. A1 - MacDonald, D. J. A1 - Owings, S. M. A1 - Hanson, T. E. A1 - Beinart, R. A. A1 - Girguis, P. R. KW - abiotic KW - biotic KW - chemolithotrophy KW - chlorobaculum tepidum KW - oxidation KW - photolithotrophy KW - sulfide AB -

The thermodynamics for the first electron transfer step for sulfide and oxygen indicates that the reaction is unfavorable as unstable superoxide and bisulfide radical ions would need to be produced. However, a two-electron transfer is favorable as stable S(0) and peroxide would be formed, but the partially filled orbitals in oxygen that accept electrons prevent rapid kinetics. Abiotic sulfide oxidation kinetics improve when reduced iron and/or manganese are oxidized by oxygen to form oxidized metals which in turn oxidize sulfide. Biological sulfur oxidation relies on enzymes that have evolved to overcome these kinetic constraints to affect rapid sulfide oxidation. Here we review the available thermodynamic and kinetic data for H2S and HS center dot as well as O-2, reactive oxygen species, nitrate, nitrite, and NOx species. We also present new kinetic data for abiotic sulfide oxidation with oxygen in trace metal clean solutions that constrain abiotic rates of sulfide oxidation in metal free solution and agree with the kinetic and thermodynamic calculations. Moreover, we present experimental data that give insight on rates of chemolithotrophic and photolithotrophic sulfide oxidation in the environment. We demonstrate that both anaerobic photolithotrophic and aerobic chemolithotrophic sulfide oxidation rates are three or more orders of magnitude higher than abiotic rates suggesting that in most environments biotic sulfide oxidation rates will far exceed abiotic rates due to the thermodynamic and kinetic constraints discussed in the first section of the paper. Such data reshape our thinking about the biotic and abiotic contributions to sulfide oxidation in the environment.

VL - 2 SN - 1664-302X N1 -

V31deTimes Cited:13Cited References Count:51

JO - Front MicrobiolFront Microbiol ER - TY - CONF T1 - Abundance and Distribution of Diagnostic Carbon Fixation Genes in a Deep-Sea Hydrothermal Gradient Ecosystem T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Blumenfeld, HN A1 - Kelley, DS A1 - Girguis, P. R. A1 - Schrenk, MO JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Autonomous in-situ qPCR in the Deep Sea T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Ussler, W. A1 - Tavormina, P. A1 - Preston, C. A1 - Shah, S A1 - Girguis, P. R. A1 - Birch, J. M. A1 - Orphan, V. A1 - Scholin, C. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Benthic microbial fuel cells: Long-term power sources for wireless marine sensor networks T2 - Sensors, and command, control, communications, and intelligence (C3I) technologies for homeland security and homeland defense IX Y1 - 2010 A1 - Guzman, Juan J A1 - Cooke, Keegan G A1 - Gay, Marcus O A1 - Radachowsky, Sage E A1 - Girguis, Peter R. A1 - Chiu, Michael A JF - Sensors, and command, control, communications, and intelligence (C3I) technologies for homeland security and homeland defense IX PB - International Society for Optics and Photonics VL - 7666 ER - TY - CONF T1 - Capitalizing on Education and Outreach (E/O) Expertise to Broaden Impacts T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Girguis, P. R. A1 - Herren, C A1 - DeCharon, A. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Correlating Community Dynamics and Microbial protein expression With Changes In Hydrothermal Chemistry At The Juan UUUFuca Ridge using Biological Osmotic Sampling Systems(BOSS) T2 - Proceedings from the 2010 AGU Ocean Sciences Meeting Y1 - 2010 A1 - Girguis, P. R. A1 - Robidart, J. A1 - Wheat, C. G. JF - Proceedings from the 2010 AGU Ocean Sciences Meeting PB - American Geophysical Union, 2000 Florida Ave., N. W. Washington DC 20009 USA, ER - TY - CONF T1 - Evidence for Hydrothermal Vents as" Biogeobatteries" T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Nielsen, M. E. A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Geological and geochemical controls on the distribution of Alviniconcha vent snail symbioses: Have we finally linked mantle to microbe? T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Girguis, P. R. A1 - Beinart, R. A1 - Sanders, J. A1 - Seewald, J JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - JOUR T1 - Harnessing energy from marine productivity using bioelectrochemical systems JF - Current Opinion in Biotechnology Y1 - 2010 A1 - Girguis, Peter R. A1 - Nielsen, Mark E A1 - Figueroa, Israel PB - Elsevier Current Trends VL - 21 IS - 3 ER - TY - CONF T1 - In Situ Stable Isotopic Detection of Anaerobic Oxidation of Methane in Monterey Bay Cold Seeps Via Off-Axis Integrated Cavity Output Spectroscopy T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Wankel, S. D. A1 - Gupta, M. A1 - Leen, J A1 - Provencal, RA A1 - Parsotam, V A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Recalibrating the concentration of Precambrian seawater sulfate T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Johnston, D. T. A1 - Bradley, A. S. A1 - Hoarfrost, A A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Abundance and Distribution of Diagnostic Carbon Fixation Genes in a Deep-Sea Hydrothermal Gradient Ecosystem T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Blumenfeld, HN A1 - Kelley, DS A1 - Girguis, P. R. A1 - Schrenk, MO JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Are hydrothermal vents natural microbial fuel cells? T2 - GEOCHIMICA ET COSMOCHIMICA ACTA Y1 - 2010 A1 - Nielsen, Mark E A1 - Girguis, Peter R. JF - GEOCHIMICA ET COSMOCHIMICA ACTA PB - PERGAMON-ELSEVIER SCIENCE LTD THE BOULEVARD, LANGFORD LANE, KIDLINGTON … VL - 74 IS - 12 ER - TY - CONF T1 - Autonomous in-situ qPCR in the Deep Sea T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Ussler, W. A1 - Tavormina, P. A1 - Preston, C. A1 - Shah, S A1 - Girguis, P. R. A1 - Birch, J. M. A1 - Orphan, V. A1 - Scholin, C. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Benthic microbial fuel cells: Long-term power sources for wireless marine sensor networks T2 - Sensors, and command, control, communications, and intelligence (C3I) technologies for homeland security and homeland defense IX Y1 - 2010 A1 - Guzman, Juan J A1 - Cooke, Keegan G A1 - Gay, Marcus O A1 - Radachowsky, Sage E A1 - Girguis, Peter R. A1 - Chiu, Michael A JF - Sensors, and command, control, communications, and intelligence (C3I) technologies for homeland security and homeland defense IX PB - International Society for Optics and Photonics VL - 7666 ER - TY - CONF T1 - Capitalizing on Education and Outreach (E/O) Expertise to Broaden Impacts T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Girguis, P. R. A1 - Herren, C A1 - DeCharon, A. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Correlating Community Dynamics and Microbial protein expression With Changes In Hydrothermal Chemistry At The Juan UUUFuca Ridge using Biological Osmotic Sampling Systems(BOSS) T2 - Proceedings from the 2010 AGU Ocean Sciences Meeting Y1 - 2010 A1 - Girguis, P. R. A1 - Robidart, J. A1 - Wheat, C. G. JF - Proceedings from the 2010 AGU Ocean Sciences Meeting PB - American Geophysical Union, 2000 Florida Ave., N. W. Washington DC 20009 USA, ER - TY - CONF T1 - Evidence for Hydrothermal Vents as" Biogeobatteries" T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Nielsen, M. E. A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Evidence for seismicity influencing deep sea primary productivity: A year-long study of microbial processes at the Juan de Fuca ridge using biological osmotic samplers T2 - GEOCHIMICA ET COSMOCHIMICA ACTA Y1 - 2010 A1 - Peter Girguis A1 - Robidart, Julie A1 - Wheat, Geoffrey A1 - Frank, Kiana JF - GEOCHIMICA ET COSMOCHIMICA ACTA PB - PERGAMON-ELSEVIER SCIENCE LTD THE BOULEVARD, LANGFORD LANE, KIDLINGTON … VL - 74 IS - 12 ER - TY - CONF T1 - Geological and geochemical controls on the distribution of Alviniconcha vent snail symbioses: Have we finally linked mantle to microbe? T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Girguis, P. R. A1 - Beinart, R. A1 - Sanders, J. A1 - Seewald, J JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - JOUR T1 - Harnessing energy from marine productivity using bioelectrochemical systems JF - Current Opinion in Biotechnology Y1 - 2010 A1 - Girguis, Peter R. A1 - Nielsen, Mark E A1 - Figueroa, Israel PB - Elsevier Current Trends VL - 21 IS - 3 ER - TY - CONF T1 - In Situ Stable Isotopic Detection of Anaerobic Oxidation of Methane in Monterey Bay Cold Seeps Via Off-Axis Integrated Cavity Output Spectroscopy T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Wankel, S. D. A1 - Gupta, M. A1 - Leen, J A1 - Provencal, RA A1 - Parsotam, V A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - ABST T1 - Linking mantle to microbe: a community-wide effort to ally hydrothermal vent microbial identity and ecology to geochemical cycles via metagenomics Y1 - 2010 A1 - Peter Girguis PB - DOE Joint Genome Institute ER - TY - ABST T1 - Methods and apparatus for stimulating and managing power from microbial fuel cells Y1 - 2010 A1 - Girguis, Peter Riad A1 - Kauffman, Peter Carr N1 - US Patent App. 12/311,165 ER - TY - JOUR T1 - New constraints on methane fluxes and rates of anaerobic methane oxidation in a Gulf of Mexico brine pool via in situ mass spectrometry JF - Deep Sea Research Part II: Topical Studies in Oceanography Y1 - 2010 A1 - Wankel, Scott D A1 - Joye, Samantha B A1 - Samarkin, Vladimir A A1 - Shah, Sunita R A1 - Friederich, Gernot A1 - Melas-Kyriazi, John A1 - Girguis, Peter R. PB - Pergamon VL - 57 IS - 21-23 ER - TY - CONF T1 - Recalibrating the concentration of Precambrian seawater sulfate T2 - AGU Fall Meeting Abstracts Y1 - 2010 A1 - Johnston, D. T. A1 - Bradley, A. S. A1 - Hoarfrost, A A1 - Girguis, P. R. JF - AGU Fall Meeting Abstracts VL - 2010 ER - TY - CONF T1 - Searching for microbes in deep-sea seep and hydrothermal vents using the Environmental Sample Processor T2 - Astrobiology Science Conference Y1 - 2010 A1 - Birch, J A1 - Preston, C. A1 - Pargett, D. A1 - Jensen, S. A1 - Roman, B. A1 - Everloce, C A1 - Marin III, R A1 - Orphan, V. A1 - Girguis, P. A1 - Scholin, C. JF - Astrobiology Science Conference VL - 201 IS - 9 ER - TY - CONF T1 - Subsurface microbial observatories to investigate the deep ocean crust biosphere- development, testing, and future T2 - Proceedings from the 2010 AGU Ocean Sciences Meeting Y1 - 2010 A1 - Edwards, Katrina J A1 - Bach, W. A1 - Cowen, JP A1 - Fisher, A A1 - Girguis, P. R. A1 - Glazer, BT A1 - Huber, JA A1 - Nealson, KH A1 - Orcutt, B A1 - Pettigrew, T A1 - others JF - Proceedings from the 2010 AGU Ocean Sciences Meeting PB - American Geophysical Union, 2000 Florida Ave., N. W. Washington DC 20009 USA, ER - TY - Generic T1 - Are hydrothermal vents natural microbial fuel cells? T2 - Geochimica Et Cosmochimica Acta Y1 - 2010 A1 - Nielsen, M. E. A1 - Girguis, P. R. JF - Geochimica Et Cosmochimica Acta VL - 74 SN - 0016-7037 N1 -

Suppl. 1676TLTimes Cited:0Cited References Count:0

JO - Geochim Cosmochim AcGeochim Cosmochim Ac ER - TY - JOUR T1 - Benthic Microbial Fuel Cells: Long-Term Power Sources for Wireless Marine Sensor Networks JF - Sensors, and Command, Control, Communications, and Intelligence (C3i) Technologies for Homeland Security and Homeland Defense Ix Y1 - 2010 A1 - Guzman, J. J. A1 - Cooke, K. G. A1 - Gay, M. O. A1 - Radachowsky, S. E. A1 - Girguis, P. R. A1 - Chiu, M. A. KW - benthic microbial fuel cell KW - energy KW - environmental fuel cell KW - marine power KW - marine sensor network KW - remote power source KW - remote sensor network AB -

Wireless marine sensor networks support an assortment of services in industries ranging from national security and defense to communications and environmental stewardship. Expansion of marine sensor networks has been inhibited by the limited availability and high cost of long-term power sources. Benthic Microbial Fuel Cells (BMFCs) are a novel form of energy harvesting for marine environments. Through research conducted in-lab and by academic collaborators, Trophos Energy has developed a series of novel BMFC architectures to improve power generation capability and overall system robustness. When integrated with Trophos' power management electronics, BMFCs offer a robust, long-term power solution for a variety of remote marine applications. The discussions provided in this paper outline the architectural evolution of BMFC technology to date, recent experimental results that will govern future BMFC designs, and the present and future applicability of BMFC systems as power sources for wireless marine sensor networks.

VL - 7666 SN - 0277-786X N1 -

Bss37Times Cited:2Cited References Count:13Proceedings of SPIE-The International Society for Optical Engineering

JO - P Soc Photo-Opt InsP Soc Photo-Opt Ins ER - TY - CHAP T1 - Fundamentals of Benthic Microbial Fuel Cells: Theory, Development, and Applications T2 - Bioelectrochemical systems Y1 - 2010 A1 - Girguis, P. R. A1 - Nielsen, M. E. A1 - Reimers, C. E. JF - Bioelectrochemical systems PB - Springer Verlag Press ER - TY - JOUR T1 - Harnessing energy from marine productivity using bioelectrochemical systems. JF - Current Opinion in Biotechnology Y1 - 2010 A1 - Girguis, P. R. A1 - Nielsen, M. E. A1 - Figueroa, I. KW - cathode materials KW - electron-transfer KW - escherichia-coli KW - generating electricity KW - iron KW - microbial fuel-cell KW - organic-matter KW - power KW - reduction KW - waste-water AB -

Over the past decade, studies have shown that devices called microbial fuel cells (MFCs) can harness electricity from microbially mediated degradation of organic carbon, in both lab cultures and natural environments. Other studies have shown that MFCs can harness power from coastal and deep ocean sediments, as well as from plankton, without any fuel supplementation or microbial inoculation. The fuel for these systems is organic matter resulting from oceanic primary productivity. Models suggest that MFCs may operate for decades on endogenous organic carbon. In light of their capacity to generate power in natural milieus by tapping into biogeochemical cycles, MFCs may one day provide an efficient means of generating power (or high value biofuels) directly from marine productivity.

VL - 21 SN - 0958-1669 N1 -

613CKTimes Cited:9Cited References Count:81

JO - Curr Opin BiotechCurr Opin Biotech ER - TY - JOUR T1 - New constraints on methane fluxes and rates of anaerobic methane oxidation in a Gulf of Mexico brine pool via in situ mass spectrometry JF - Deep-Sea Research Part II -Topical Studies in Oceanography Y1 - 2010 A1 - Wankel, S. D. A1 - Joye, S. B. A1 - Samarkin, V. A. A1 - Shah, S. R. A1 - Friederich, G. A1 - Melas-Kyriazi, J. A1 - Girguis, P. R. KW - biogeochemistry KW - brine pool KW - cold seeps KW - dissolved-gases KW - gas hydrate KW - gulf of mexico KW - mass spectrometer KW - methane flux KW - methane oxidation KW - riftia-pachyptila KW - saanich inlet KW - sea-floor brines KW - sediments KW - slope KW - sulfate reduction AB -

Deep-sea biogeochemical cycles are, in general, poorly understood owing to the difficulties of making measurements in situ, recovering samples with minimal perturbation, and, in many cases, coping with high spatial and temporal heterogeneity. In particular, biogeochemical fluxes of volatiles such as methane remain largely unconstrained because of the difficulties with accurate quantification in situ and the patchiness of point sources such as seeps and brine pools. To better constrain biogeochemical fluxes and cycling, we have developed a deep-sea in situ mass spectrometer (ISMS) to enable high-resolution quantification of volatiles in situ. Here we report direct measurements of methane concentrations made in a Gulf of Mexico brine pool located at a depth of over 2300 m. Concentrations of up to 33 mM methane were observed within the brine pool, whereas concentrations in the water directly above were three orders of magnitude lower. These direct measurements enabled us to make the first accurate estimates of the diffusive flux from a brine pool, calculated to be 1.1 +/- 0.2 mol m(-2) yr(-1). Integrated rate measurements of aerobic methane oxidation in the water column overlying the brine pool were similar to 320 mu mol m(-2) yr(-1), accounting at most for just 0.03% of the diffusive methane flux from the brine pool. Calculated rates of anaerobic methane oxidation were 600-1200 mu M yr(-1), one to two orders of magnitude higher than previously published values of AOM in anoxic fluids. These findings suggest that brine pools are enormous point sources of methane in the deep sea, and may, in aggregate, have a pronounced impact on the global marine methane cycle. (C) 2010 Elsevier Ltd. All rights reserved.

VL - 57 SN - 0967-0645 N1 -

694TZTimes Cited:21Cited References Count:52

JO - Deep-Sea Res Pt IiDeep-Sea Res Pt Ii ER - TY - JOUR T1 - Coupling metabolite flux to proteomics: Insights into the molecular mechanisms underlying primary productivity by tubeworm symbionts JF - Geochimica et Cosmochimica Acta Supplement Y1 - 2009 A1 - Girguis, Peter Riad A1 - Robidart, Julie A1 - Nyholm, Spencer VL - 73 ER - TY - JOUR T1 - Methane cycling at Lost City: A biological view of geochemistry JF - Geochimica et Cosmochimica Acta Supplement Y1 - 2009 A1 - Bradley, Alexander S A1 - Song, Pengfei A1 - Summons, Roger E A1 - Girguis, Peter R. VL - 73 ER - TY - JOUR T1 - Variation in evolved “limits to life” preclude universal tolerance indices: a critique of the “Respiration Index” JF - Science Y1 - 2009 A1 - Seibel, Brad A A1 - Girguis, Peter R. A1 - Childress, James J. ER - TY - CONF T1 - Bacterial community composition in hydrothermal plume environments is heterogeneous and distinct T2 - AGU Fall Meeting Abstracts Y1 - 2009 A1 - Sylvan, J. B. A1 - Toner, B. M. A1 - Girguis, P. R. A1 - Edwards, K. J. JF - AGU Fall Meeting Abstracts VL - 2009 ER - TY - JOUR T1 - Coupling metabolite flux to proteomics: Insights into the molecular mechanisms underlying primary productivity by tubeworm symbionts JF - Geochimica et Cosmochimica Acta Supplement Y1 - 2009 A1 - Girguis, Peter Riad A1 - Robidart, Julie A1 - Nyholm, Spencer VL - 73 ER - TY - CONF T1 - Exploring the Boundaries of Metazoan Thermotolerance at Hydrothermal Vents: Respiration and Protein Expression of Paralvinellid Worms T2 - INTEGRATIVE AND COMPARATIVE BIOLOGY Y1 - 2009 A1 - Dilly, G. F. A1 - Girguis, P. R. JF - INTEGRATIVE AND COMPARATIVE BIOLOGY PB - OXFORD UNIV PRESS INC JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA VL - 49 ER - TY - CONF T1 - From metabolite flux to gene expression and proteomics: insights into the molecular mechanisms underlying primary productivity in hydrothermal vent tubeworms T2 - INTEGRATIVE AND COMPARATIVE BIOLOGY Y1 - 2009 A1 - Girguis, P. R. A1 - Nyholm, S. A1 - Robidart, J. A. A1 - Girguis JF - INTEGRATIVE AND COMPARATIVE BIOLOGY PB - OXFORD UNIV PRESS INC JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA VL - 49 ER - TY - JOUR T1 - Influence of substrate on electron transfer mechanisms in chambered benthic microbial fuel cells JF - Environmental science & technology Y1 - 2009 A1 - Nielsen, Mark E A1 - Wu, Di M A1 - Girguis, Peter R. A1 - Reimers, Clare E PB - American Chemical Society VL - 43 IS - 22 ER - TY - JOUR T1 - Methane cycling at Lost City: A biological view of geochemistry JF - Geochimica et Cosmochimica Acta Supplement Y1 - 2009 A1 - Bradley, Alexander S A1 - Song, Pengfei A1 - Summons, Roger E A1 - Girguis, Peter R. VL - 73 ER - TY - ABST T1 - Peering Into the Private Lives of Microbes: From Microbial Activity to Geochemical Cycles Y1 - 2009 A1 - Peter Girguis PB - Organismic & Evolutionary Biology, Harvard University ER - TY - JOUR T1 - Quantitative population dynamics of microbial communities in plankton-fed microbial fuel cells JF - The ISME journal Y1 - 2009 A1 - White, Helen K A1 - Reimers, Clare E A1 - Cordes, Erik E A1 - Dilly, Geoffrey F A1 - Girguis, Peter R. PB - Nature Publishing Group VL - 3 IS - 6 ER - TY - JOUR T1 - Real-time in situ volatile characterization and methane carbon stable isotopic composition in the deep sea JF - Geochimica et Cosmochimica Acta Supplement Y1 - 2009 A1 - Wankel, Scott D A1 - Lilley, Marvin D A1 - Olson, Eric A1 - Girguis, Peter R. VL - 73 ER - TY - JOUR T1 - Thiotaurine and hypotaurine contents in hydrothermal-vent polychaetes without thiotrophic endosymbionts: correlation With sulfide exposure JF - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology Y1 - 2009 A1 - Yancey, Paul H A1 - Ishikawa, Joanne A1 - Meyer, Brigitte A1 - Girguis, Peter R. A1 - Lee, Raymond W PB - Wiley Subscription Services, Inc., A Wiley Company Hoboken VL - 311 IS - 6 ER - TY - JOUR T1 - Variation in evolved “limits to life” preclude universal tolerance indices: a critique of the “Respiration Index” JF - Science Y1 - 2009 A1 - Seibel, Brad A A1 - Girguis, Peter R. A1 - Childress, James J. ER - TY - Generic T1 - Coupling metabolite flux to proteomics: Insights into the molecular mechanisms underlying primary productivity by tubeworm symbionts T2 - Geochimica Et Cosmochimica Acta Y1 - 2009 A1 - Girguis, P. R. A1 - Robidart, J. A1 - Nyholm, S. JF - Geochimica Et Cosmochimica Acta VL - 73 SN - 0016-7037 N1 -

460YSTimes Cited:0Cited References Count:0

JO - Geochim Cosmochim AcGeochim Cosmochim Ac ER - TY - Generic T1 - Exploring the Boundaries of Metazoan Thermotolerance at Hydrothermal Vents: Respiration and Protein Expression of Paralvinellid Worms T2 - Integrative and Comparative Biology Y1 - 2009 A1 - Dilly, G. F. A1 - Girguis, P. R. JF - Integrative and Comparative Biology VL - 49 SN - 1540-7063 N1 -

481KLTimes Cited:0Cited References Count:0

JO - Integr Comp BiolIntegr Comp Biol ER - TY - JOUR T1 - From metabolite flux to gene expression and proteomics: insights into the molecular mechanisms underlying primary productivity in hydrothermal vent tubeworms JF - Integrative and Comparative Biology Y1 - 2009 A1 - Girguis, P. R. A1 - Nyholm, S. V. A1 - Robidart, J. A. A1 - Girguis VL - 49 SN - 1540-7063 N1 -

481KLTimes Cited:0Cited References Count:0

JO - Integr Comp BiolIntegr Comp Biol ER - TY - JOUR T1 - Influence of Substrate on Electron Transfer Mechanisms in Chambered Benthic Microbial Fuel Cells. JF - Environmental Science & Technology Y1 - 2009 A1 - Nielsen, M. E. A1 - Wu, D. M. A1 - Girguis, P. R. A1 - Reimers, C. E. KW - biofilm KW - communities KW - energy-metabolism KW - harvesting electricity KW - hydrogen-sulfide KW - marine-sediments KW - oxidation KW - reduction KW - sea-floor KW - sulfate-reducing bacteria AB -

This research investigated whether the addition of an exogenous electron donor would affect power production in laboratory-scale benthic microbial fuel cells (BMFC) by differentially influencing microbially mediated electron transfer processes. Six BMFCs were operated for over one year in a temperature-controlled laboratory. Three BMFCs relied on endogenous electron donors, and three were supplemented with lactate. The supplemented BMFCs generated more cumulative charge, but did not generate higher average current between periods of lactate enrichment Coulombic efficiencies during the lactate treatments ranged from 25 to 65% suggesting that lactate utilization was variably coupled to power production. Cumulative electron flux resulting from lactate additions and chemical changes within the anode chamber, as well as a difference in the anode-hosted microbial communities indicated that lactate supplementation promoted sulfate reduction. After the addition of molybdate to suppress sulfate reduction and sulfur disproportionation, all BMFCs continued to produce current but no longer responded to lactate additions. Chemical data support a two-step cycle in which endogenous organic carbon and/or supplemented lactate fuel sulfate reduction resulting in sulfide and simple organic molecules (such as acetate) that can act as the electron donors for the BMFC.

VL - 43 SN - 0013-936X N1 -

516ZVTimes Cited:22Cited References Count:45

JO - Environ Sci TechnolEnviron Sci Technol ER - TY - Generic T1 - Methane cycling at Lost City: A biological view of geochemistry T2 - Geochimica Et Cosmochimica Acta Y1 - 2009 A1 - Bradley, A. S. A1 - Song, P. A1 - Summons, R. E. A1 - Girguis, P. R. KW - hydrothermal field JF - Geochimica Et Cosmochimica Acta VL - 73 SN - 0016-7037 N1 -

460YSTimes Cited:0Cited References Count:4

JO - Geochim Cosmochim AcGeochim Cosmochim Ac ER - TY - JOUR T1 - Quantitative population dynamics of microbial communities in plankton-fed microbial fuel cells. JF - Isme Journal Y1 - 2009 A1 - White, H. K. A1 - Reimers, C. E. A1 - Cordes, E. E. A1 - Dilly, G. F. A1 - Girguis, P. R. KW - bacterial communities KW - cell KW - diversity KW - Ecology KW - electricity KW - electron-transfer KW - energy KW - fuel KW - generation KW - plankton KW - power KW - real-time pcr KW - reduction KW - sediments AB -

This study examines changes in diversity and abundance of bacteria recovered from the anodes of microbial fuel cells (MFCs) in relation to anode potential, power production and geochemistry. MFCs were batch-fed with plankton, and two systems were maintained at different potentials whereas one was at open circuit for 56.8 days. Bacterial phylogenetic diversity during peak power was assessed from 16S rDNA clone libraries. Throughout the experiment, microbial community structure was examined using terminal restriction fragment length polymorphism. Changes in cell density of key phylotypes, including representatives of delta-, epsilon-, gamma-proteobacteria and Flavobacterium-Cytophaga-Bacteroides, were enumerated by quantitative PCR. Marked differences in phylogenetic diversity were observed during peak power versus the final time point, and changes in microbial community structure were strongly correlated to dissolved organic carbon and ammonium concentrations within the anode chambers. Community structure was notably different between the MFCs at different anode potentials during the onset of peak power. At the final time point, however, the anode-hosted communities in all MFCs were similar. These data demonstrate that differences in growth, succession and population dynamics of key phylotypes were due to anode potential, which may relate to their ability to exploit the anode as an electron acceptor. The geochemical milieu, however, governs overall community diversity and structure. These differences reflect the physiological capacity of specific phylotypes to catabolize plankton-derived organic matter and exploit the anode of an MFC for their metabolism directly or indirectly through syntrophy. The ISME Journal (2009) 3, 635-646; doi: 10.1038/ismej.2009.12; published online 26 February 2009

VL - 3 SN - 1751-7362 N1 -

452HITimes Cited:25Cited References Count:31

JO - Isme JIsme J ER - TY - Generic T1 - Real-time in situ volatile characterization and methane carbon stable isotopic composition in the deep sea T2 - Geochimica Et Cosmochimica Acta Y1 - 2009 A1 - Wankel, S. D. A1 - Lilley, M. D. A1 - Olson, E. A1 - Girguis, P. R. JF - Geochimica Et Cosmochimica Acta VL - 73 SN - 0016-7037 N1 -

460YSTimes Cited:0Cited References Count:0

JO - Geochim Cosmochim AcGeochim Cosmochim Ac ER - TY - JOUR T1 - Thiotaurine and Hypotaurine Contents in Hydrothermal-Vent Polychaetes Without Thiotrophic Endosymbionts: Correlation With Sulfide Exposure. JF - Journal of Experimental Zoology Part a-Ecological Genetics and Physiology Y1 - 2009 A1 - Yancey, P. H. A1 - Ishikawa, J. A1 - Meyer, B. A1 - Girguis, P. R. A1 - Lee, R. W. KW - amino-acids KW - cold seeps KW - de-fuca ridge KW - hydrostatic-pressure KW - northeast pacific KW - organic osmolytes KW - riftia-pachyptila KW - sea animals KW - symbiotic bacteria KW - tolerance AB -

Invertebrates at hydrothermal vents and cold seeps must cope with toxic H(2)S. One proposed protection mechanism involves taurine derivatives: At vents and seeps, many animals have high levels of hypotaurine and thiotaurine (a product of hypotaurine and HS), originally found in animals with thiotrophic endosymbionts. To further test the role of these compounds, we analyzed them in vent polychaetes without endosymbionts: Paralvinella sulfincola, P. palmiformis and P. pandorae (paralvinellids) and Nicomache venticola (maldanid). P. sulfincola were collected from a high temperature (42-68 degrees C) and a warm site (21-35 degrees C). P. plamiformis and pandorae were from cool sites (12-18 degrees C) and N. venticola were from a cold site (4 degrees C). H(2)S concentrations in vent effluent largely correlate with temperature. Some specimens were frozen; other ones were kept alive in laboratory chambers, with and without sulfide. Tissues were analyzed for taurine derivatives and other solutes that serve as organic osmolytes. The major osmolyte of all species was glycine. Thiotaurine contents were significantly different among all species, in the order P. sulfincola hot > P. sulfincola warm > P. pandorae > P. palmiformis > N. venticola. P. sulfincola also had high levels of sarcosine; others species had none. Sarcosine and hypotaurine contents of P. sulfincola's branchiae were higher, while glycine contents were lower, than in main body. In P. palmiformis kept in pressure chambers with sulfide, thiotaurine contents were higher and hypotaurine lower than in those. kept without sulfide. These results support the hypothesis that conversion of hypotaurine to thiotaurine detoxifies sulfide in vent animals without endosymbionts. J. Exp. Zool. 311A:439-447, 2009, (C) 2009 Wiley-Liss, Inc.

VL - 311A SN - 1932-5223 N1 -

473VXTimes Cited:4Cited References Count:46

JO - J Exp Zool Part AJ Exp Zool Part A ER - TY - ABST T1 - Variation in evolved limits to life preclude universal tolerance indices: a critque of the Respiration Index Y1 - 2009 A1 - Seibel, B. A. A1 - Girguis, P. R. A1 - Childress, J. J. JF - Science e-Letters ER - TY - JOUR T1 - Hydrothermal vents and eukaryotic thermotolerance: Profiling heat shock proteins expression in Paralvinella sulfincola JF - Comparative Biochemistry and Physiology, Part A Y1 - 2008 A1 - Dilly, G A1 - Girguis, P. VL - 1 IS - 151 ER - TY - CONF T1 - Iron, Sulfur, Arsenic and Water: Geochemical Implications of Facultative Anoxygenic Photosynthesis in Cyanobacteria and the Slow Rise of Oxygen T2 - AGU Fall Meeting Abstracts Y1 - 2008 A1 - Wolfe-Simon, F A1 - Johnston, D. T. A1 - Girguis, P. R. A1 - Pearson, A A1 - Knoll, A. H. JF - AGU Fall Meeting Abstracts VL - 2008 ER - TY - CONF T1 - The BOSS: a novel approach to coupling temporal changes in geochemistry and microbiology in the deep subsurface biosphere. T2 - AGU Fall Meeting Abstracts Y1 - 2008 A1 - Girguis, P. R. A1 - Robidart, J. A1 - Wheat, G JF - AGU Fall Meeting Abstracts VL - 2008 ER - TY - JOUR T1 - Comparative genomics of vesicomyid clam (Bivalvia: Mollusca) chemosynthetic symbionts JF - BMC genomics Y1 - 2008 A1 - Newton, Irene LG A1 - Girguis, Peter R. A1 - Cavanaugh, Colleen M PB - BioMed Central VL - 9 IS - 1 ER - TY - JOUR T1 - Coupling metabolite flux to transcriptomics: insights into the molecular mechanisms underlying primary productivity by the hydrothermal vent tubeworm Ridgeia piscesae JF - The Biological Bulletin Y1 - 2008 A1 - Nyholm, Spencer V. A1 - Robidart, Julie A1 - Girguis, Peter R. PB - Marine Biological Laboratory VL - 214 IS - 3 ER - TY - CONF T1 - Earth's Deep Biosphere and Life in the Solar System T2 - AGU Fall Meeting Abstracts Y1 - 2008 A1 - Schulte, M A1 - Orcutt, B A1 - Girguis, P. JF - AGU Fall Meeting Abstracts VL - 2008 ER - TY - JOUR T1 - Hydrothermal vents and eukaryotic thermotolerance: Profiling heat shock proteins expression in Paralvinella sulfincola JF - Comparative Biochemistry and Physiology, Part A Y1 - 2008 A1 - Dilly, G A1 - Girguis, P. VL - 1 IS - 151 ER - TY - CONF T1 - Iron, Sulfur, Arsenic and Water: Geochemical Implications of Facultative Anoxygenic Photosynthesis in Cyanobacteria and the Slow Rise of Oxygen T2 - AGU Fall Meeting Abstracts Y1 - 2008 A1 - Wolfe-Simon, F A1 - Johnston, D. T. A1 - Girguis, P. R. A1 - Pearson, A A1 - Knoll, A. H. JF - AGU Fall Meeting Abstracts VL - 2008 ER - TY - CONF T1 - Power Production by Sediment-Hosted Microbial Fuel Cells: The Influence of Substrate Availability and Microbial Ecology T2 - 2008 Joint Meeting of The Geological Society of America, Soil Science Society of America, American Society of Agronomy, Crop Science Society of America, Gulf Coast Association of Geological Societies with the Gulf Coast Section of SEPM Y1 - 2008 A1 - Girguis, Peter R. JF - 2008 Joint Meeting of The Geological Society of America, Soil Science Society of America, American Society of Agronomy, Crop Science Society of America, Gulf Coast Association of Geological Societies with the Gulf Coast Section of SEPM ER - TY - JOUR T1 - Sustainable energy from deep ocean cold seeps JF - Energy & Environmental Science Y1 - 2008 A1 - Nielsen, Mark E A1 - Reimers, Clare E A1 - White, Helen K A1 - Sharma, Sonam A1 - Girguis, Peter R. PB - Royal Society of Chemistry VL - 1 IS - 5 ER - TY - JOUR T1 - Comparative genomics of vesicomyid clam (Bivalvia: Mollusca) chemosynthetic symbionts JF - Bmc Genomics Y1 - 2008 A1 - Newton, I. L. G. A1 - Girguis, P. R. A1 - Cavanaugh, C. M. KW - blochmannia KW - buchnera-aphidicola KW - calyptogena-magnifica KW - deep-sea clam KW - endosymbiotic bacteria KW - Evolution KW - hydrothermal vent KW - nitrate reductase KW - sequence KW - transmission AB -

Background: The Vesicomyidae (Bivalvia: Mollusca) are a family of clams that form symbioses with chemosynthetic gamma-proteobacteria. They exist in environments such as hydrothermal vents and cold seeps and have a reduced gut and feeding groove, indicating a large dependence on their endosymbionts for nutrition. Recently, two vesicomyid symbiont genomes were sequenced, illuminating the possible nutritional contributions of the symbiont to the host and making genome-wide evolutionary analyses possible.Results: To examine the genomic evolution of the vesicomyid symbionts, a comparative genomics framework, including the existing genomic data combined with heterologous microarray hybridization results, was used to analyze conserved gene content in four vesicomyid symbiont genomes. These four symbionts were chosen to include a broad phylogenetic sampling of the vesicomyid symbionts and represent distinct chemosynthetic environments: cold seeps and hydrothermal vents.Conclusion: The results of this comparative genomics analysis emphasize the importance of the symbionts' chemoautotrophic metabolism within their hosts. The fact that these symbionts appear to be metabolically capable autotrophs underscores the extent to which the host depends on them for nutrition and reveals the key to invertebrate colonization of these challenging environments.

VL - 9 SN - 1471-2164 N1 -

417VSTimes Cited:13Cited References Count:62

JO - Bmc GenomicsBmc Genomics ER - TY - JOUR T1 - Coupling metabolite flux to transcriptomics: Insights into the molecular mechanisms underlying primary productivity by the hydrothermal vent tubeworm Ridgeia piscesae JF - Biological Bulletin Y1 - 2008 A1 - Nyholm, S. V. A1 - Robidart, J. A1 - Girguis, P. R. KW - alvinella-pompejana KW - carbonic-anhydrase KW - chemoautotrophic symbionts KW - community KW - deep-sea vents KW - east pacific rise KW - fuca ridge KW - methanococcus-jannaschii KW - sulfide binding KW - worm riftia-pachyptila AB -

Deep-sea hydrothermal vents host highly productive ecosystems. Many of these communities are dominated by vestimentiferan tubeworms that house endosymbiotic chemoautotrophic bacteria that provide the hosts with their primary nutritional needs. Rates of carbon fixation by these symbioses are also among the highest recorded. Despite the breadth of physiological and biochemical research on these associations, the underlying molecular mechanisms that regulate host and symbiont metabolite flux and carbon fixation are largely unknown. Here we present metabolite flux and transcriptomics data from shipboard high-pressure respirometry experiments in which we maintained Ridgeia piscesae tubeworms at conditions comparable to those in situ. Host trophosome was used for cDNA library construction and sequencing. Of the 19,132 clones sequenced, 10,684 represented unique expressed sequence tags (ESTs). The highest proportions of genes are involved with translation, ribosomal structure and biogenesis, cellular processing, and signal transduction. There was moderate representation of genes involved in metabolite exchange and acid-base regulation. These data represent the first concomitant surveys of metabolite flux rates and gene expression for a chemoautotrophic symbiosis during net autotrophy, and they suggest that-in the case of Riageia piscesae-host-symbiont interactions such as cell cycle regulation may play a significant role in maintaining physiological poise during high productivity.

VL - 214 SN - 0006-3185 N1 -

319TOTimes Cited:13Cited References Count:73

JO - Biol BullBiol Bull-Us ER - TY - JOUR T1 - Sustainable energy from deep ocean cold seeps JF - Energy & Environmental Science Y1 - 2008 A1 - Nielsen, M. E. A1 - Reimers, C. E. A1 - White, H. K. A1 - Sharma, S. A1 - Girguis, P. R. KW - california KW - communities KW - electron-transfer KW - geobacter-sulfurreducens KW - harvesting electricity KW - microbial fuel-cells KW - monterey bay KW - power KW - sea-floor KW - sediment-water interface AB -

Two designs of benthic microbial fuel cell (BMFC) were deployed at cold seeps in Monterey Canyon, CA, unattended for between 68 and 162 days. One design had a cylindrical solid graphite anode buried vertically in sediment, and the other had a carbon fiber brush anode semi-enclosed in a chamber above the sediment-water interface. Each chamber included two check valves to allow fluid flow from the sediment into the chamber. On average, power outputs were 0.2 mW (32 mW m(-2) normalized to cross sectional area) from the solid anode BMFC and from 11 to 56 mW (27-140 mW m(-2)) during three deployments of the chambered design. The range in power produced with the chambered BMFC was due to different valve styles, which appear to have permitted different rates of chemical seepage from the sediments into the anode chamber. Valves with the lowest breaking pressure led to the highest power production and presumably the highest inputs of electron donors. The increase in power coincided with a significant change in the microbial community associated with the anode from being dominated by epsilonproteobacteria to a more diverse community with representatives from deltaproteobacteria, epsilonproteobacteria, firmicutes, and flavobacterium/cytophaga/bacterioides (FCB). The highest levels of power delivered by the chambered BMFC would meet the energy requirements of many oceanographic sensors marketed today. In addition, these BMFCs did not exhibit signs of electrochemical passivation or progressive substrate depletion as is often observed with buried anodes.

VL - 1 SN - 1754-5692 N1 -

414TOTimes Cited:26Cited References Count:46

JO - Energ Environ SciEnerg Environ Sci ER - TY - CONF T1 - Towards Determining the Upper Temperature Limit to Life T2 - AGU Fall Meeting Abstracts Y1 - 2007 A1 - Kelley, DS A1 - Girguis, P. R. A1 - Wheat, G A1 - Cordes, E A1 - Schrenk, MO A1 - Lin, M A1 - Baross, JA A1 - Delaney, JR JF - AGU Fall Meeting Abstracts VL - 2007 ER - TY - CONF T1 - Tracing The Fate Of Methane-Derived Carbon: Insights Into The Ecological Physiology Of Anaerobic Methanotrophy Via Quantitative Molecular and Geochemical Approaches T2 - AGU Fall Meeting Abstracts Y1 - 2007 A1 - Girguis, P. R. A1 - Nyholm, S. V. A1 - Delong, E. F. JF - AGU Fall Meeting Abstracts VL - 2007 ER - TY - JOUR T1 - Examining the efficiency and biochemistry of plankton-fed microbial fuel cells Y1 - 2007 A1 - White, Helen K A1 - Reimers, Clare E A1 - Stecher III, HA A1 - Girguis, P. R. ER - TY - CONF T1 - Examining the efficiency and biogeochemistry of plankton-fed microbial fuel cells T2 - ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY Y1 - 2007 A1 - Reimers, Clare E A1 - Stecher III, Hilmar A A1 - White, Helen K A1 - Peter Girguis JF - ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY PB - AMER CHEMICAL SOC 1155 16TH ST, NW, WASHINGTON, DC 20036 USA VL - 234 ER - TY - CONF T1 - Ocean Microbial Fuel Cell: Power Source and Research Tool for Studying Marine Biogeochemistry T2 - AGU Spring Meeting Abstracts Y1 - 2007 A1 - Reimers, C. E. A1 - Girguis, P. A1 - Westall, J. C. A1 - Nielsen, M. E. JF - AGU Spring Meeting Abstracts VL - 2007 ER - TY - JOUR T1 - A proteomic snapshot of life at a vent JF - science Y1 - 2007 A1 - Fisher, Charles R A1 - Peter Girguis PB - American Association for the Advancement of Science VL - 315 IS - 5809 ER - TY - JOUR T1 - Substrate degradation kinetics, microbial diversity, and current efficiency of microbial fuel cells supplied with marine plankton JF - Applied and Environmental Microbiology Y1 - 2007 A1 - Reimers, Clare E A1 - Stecher III, Hilmar A A1 - Westall, John C A1 - Alleau, Yvan A1 - Howell, Kate A A1 - Soule, Leslie A1 - White, Helen K A1 - Girguis, Peter R. PB - American Society for Microbiology VL - 73 IS - 21 ER - TY - CONF T1 - Towards Determining the Upper Temperature Limit to Life T2 - AGU Fall Meeting Abstracts Y1 - 2007 A1 - Kelley, DS A1 - Girguis, P. R. A1 - Wheat, G A1 - Cordes, E A1 - Schrenk, MO A1 - Lin, M A1 - Baross, JA A1 - Delaney, JR JF - AGU Fall Meeting Abstracts VL - 2007 ER - TY - CONF T1 - Tracing The Fate Of Methane-Derived Carbon: Insights Into The Ecological Physiology Of Anaerobic Methanotrophy Via Quantitative Molecular and Geochemical Approaches T2 - AGU Fall Meeting Abstracts Y1 - 2007 A1 - Girguis, P. R. A1 - Nyholm, S. V. A1 - Delong, E. F. JF - AGU Fall Meeting Abstracts VL - 2007 ER - TY - JOUR T1 - Microbiology. A proteomic snapshot of life at a vent JF - Science Y1 - 2007 A1 - Fisher, C. R. A1 - Girguis, P. KW - *Ecosystem KW - *Proteomics KW - *Symbiosis KW - Animals KW - Bacterial Proteins/analysis/*metabolism KW - Carbon Isotopes/analysis KW - Carbon/metabolism KW - Chemoautotrophic Growth KW - Citric Acid Cycle KW - Gammaproteobacteria/*metabolism KW - Metabolic Networks and Pathways KW - Oxidation-Reduction KW - Pacific Ocean KW - Polychaeta/*microbiology/*physiology KW - Proteome KW - Reproduction KW - Sulfides/metabolism KW - Temperature VL - 315 SN - 1095-9203 (Electronic)0036-8075 (Linking) N1 -

Fisher, Charles RGirguis, PeterengCommentNew York, N.Y.2007/01/16 09:00Science. 2007 Jan 12;315(5809):198-9.

JO - ScienceScience ER - TY - JOUR T1 - Substrate degradation kinetics, microbial diversity, and current efficiency of microbial fuel cells supplied with marine plankton. JF - Applied and Environmental Microbiology Y1 - 2007 A1 - Reimers, C. E. A1 - Stecher, H. A. A1 - Westall, J. C. A1 - Alleau, Y. A1 - Howell, K. A. A1 - Soule, L. A1 - White, H. K. A1 - Girguis, P. R. KW - communities KW - electricity-generation KW - energy KW - hydrogen-sulfide KW - methane production KW - organic-matter KW - phytoplankton KW - sea-floor KW - sediments KW - sulfate reduction AB -

The decomposition of marine plankton in two-chamber, seawater-filled microbial fuel cells (MFCs) has been investigated and related to resulting chemical changes, electrode potentials, current efficiencies, and microbial diversity. Six experiments were run at various discharge potentials, and a seventh served as an open-circuit control. The plankton consisted of a mixture of freshly captured phytoplankton and zooplankton (0.21 to 1 mm) added at an initial batch concentration of 27.5 mmol liter(-1) particulate organic carbon (OC). After 56.7 days, between 19.6 and 22.2% of the initial OC remained, sulfate reduction coupled to OC oxidation accounted for the majority of the OC that was degraded, and current efficiencies (of the active MFCs) were between 11.3 and 15.5%. In the open-circuit control cell, anaerobic plankton decomposition (as quantified by the decrease in total OQ could be modeled by three terms: two first-order reaction rate expressions (0.79 day(-1) and 0.037 day(-1), at 15 degrees C) and one constant, no-reaction term (representing 10.6% of the initial 0Q. However, in each active MFC, decomposition rates increased during the third week, lagging just behind periods of peak electricity generation. We interpret these decomposition rate changes to have been due primarily to the metabolic activity of sulfur-reducing microorganisms at the anode, a finding consistent with the electrochemical oxidization of sulfide to elemental sulfur and the elimination of inhibitory effects of dissolved sulfide. Representative phylotypes, found to be associated with anodes, were allied with Delta-, Epsilon-, and Gamma-proteobacteria as well as the Flavobacterium-Cytophaga-Bacteroides and Fusobacteria. Based upon these results, we posit that higher current efficiencies can be achieved by optimizing plankton-fed MFCs for direct electron transfer from organic matter to electrodes, including microbial precolonization of high-surface-area electrodes and pulsed flowthrough additions of biomass.

VL - 73 SN - 0099-2240 IS - 21 N1 -

228AZTimes Cited:34Cited References Count:52

JO - Appl Environ MicrobAppl Environ Microb ER - TY - JOUR T1 - Metabolite uptake, stoichiometry and chemoautotrophic function of the hydrothermal vent tubeworm Riftia pachyptila: responses to environmental variations in substrate concentrations and temperature JF - Journal of experimental biology Y1 - 2006 A1 - Girguis, Peter R. A1 - Childress, James J. PB - Company of Biologists VL - 209 IS - 18 ER - TY - JOUR T1 - Microbial fuel cell energy from an ocean cold seep JF - Geobiology Y1 - 2006 A1 - Reimers, C. E. A1 - Girguis, P. A1 - Stecher, H. A. A1 - Tender, L. M. A1 - Ryckelynck, N. A1 - Whaling, P. PB - Blackwell Publishing Ltd Oxford, UK VL - 4 IS - 2 ER - TY - CONF T1 - Power storage and conversion from an ocean microbial energy source T2 - OCEANS 2006 Y1 - 2006 A1 - McBride, LR A1 - Girguis, P. A1 - Reimers, C. E. JF - OCEANS 2006 PB - IEEE ER - TY - JOUR T1 - Thermal preference and tolerance of alvinellids JF - Science Y1 - 2006 A1 - Girguis, Peter R. A1 - Lee, Raymond W PB - American Association for the Advancement of Science VL - 312 IS - 5771 ER - TY - JOUR T1 - Power Storage and Conversion from an Ocean Microbial Energy Source. JF - Proceedings of the Marine Technology Society / Institute of Electrical and Electronics Engineers Y1 - 2006 A1 - McBride, L. R. A1 - Girguis, P. R. A1 - Reimers, C. E. ER - TY - JOUR T1 - Metabolite uptake, stoichiometry and chemoautotrophic function of the hydrothermal vent tubeworm Riftia pachyptila: responses to environmental variations in substrate concentrations and temperature JF - Journal of Experimental Biology Y1 - 2006 A1 - Girguis, P. R. A1 - Childress, J. J. KW - autotrophic carbon fixation KW - bacterial symbiont KW - chemoautotrophy KW - habitats KW - hydrothermal vent KW - inorganic carbon KW - jones KW - metabolism KW - oxidation KW - riftia KW - rose garden KW - stoichiometry KW - sulfide-binding KW - symbiosis KW - vestimentifera KW - worm AB -

The hydrothermal vent tubeworm Riftia pachyptila is a dominant member of many hydrothermal vent communities along the East Pacific rise and is one of the fastest growing metazoans known. Riftia flourish in diffuse hydrothermal fluid flows, an environment with high spatial and temporal heterogeneity in physical and chemical conditions. To date, physiological and biochemical studies of Riftia have focused on Riftia's adaptations to its chemoautotrophic bacterial symbionts. However the relation between in situ physico-chemical heterogeneity and Riftia host and symbiont metabolism, in particular symbiont chemoautotrophic function, remain poorly understood. Accordingly, we conducted experiments using shipboard high-pressure respirometers to ascertain the effect of varying substrate concentrations and temperature on Riftia metabolite uptake and symbiont carbon fixation. Our results show that substrate concentrations can strongly govern Riftia oxygen and sulfide uptake rates, as well as net carbon uptake (which is a proxy for chemoautotrophic primary production). However, after sufficient exposure to sulfide and oxygen, Riftia were capable of sustaining symbiont autotrophic function for several hours in seawater devoid of sulfide or oxygen, enabling the association to support symbiont metabolism through brief periods of substrate deficiency. Overall, temperature had the largest influence on Riftia metabolite uptake and symbiont autotrophic metabolism. In sum, while Riftia requires sufficient availability of substrates to support symbiont chemoautotrophic function, it is extremely well poised to buffer the temporal and spatial heterogeneity in environmental substrate concentrations, alleviating the influence of environmental heterogeneity on symbiont chemoautotrophic function.

VL - 209 SN - 0022-0949 N1 -

079CXTimes Cited:27Cited References Count:47

ER - TY - JOUR T1 - Microbial fuel cell energy from an ocean cold seep JF - Geobiology Y1 - 2006 A1 - Reimers, C. E. A1 - Girguis, P. R. A1 - Stecher, H. A. A1 - Tender, L. M. A1 - Ryckelynck, N. A1 - Whaling, P. VL - 4 JO - Geobiology ER - TY - JOUR T1 - Thermal preference and tolerance of alvinellids JF - Science Y1 - 2006 A1 - Girguis, P. R. A1 - Lee, R. W. KW - pompeii worms KW - Temperature VL - 312 SN - 0036-8075 N1 -

032HKTimes Cited:36Cited References Count:10

JO - ScienceScience ER - TY - JOUR T1 - Using electrochemical methods to study redox processes and harvest energy from marine sediments JF - Geochim Cosmochim Acta Y1 - 2005 A1 - Reimers, C A1 - Girguis, P. A1 - Westall, J A1 - Newman, D A1 - Stecher, H A1 - Howell, K A1 - Alleau, Y. VL - 69 ER - TY - CONF T1 - Electrochemical power generation and microbial communities at seafloor seeps T2 - ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY Y1 - 2005 A1 - Reimers, C. E. A1 - Hilmar, AS A1 - Girguis, P. A1 - Tender, L. M. JF - ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY PB - AMER CHEMICAL SOC 1155 16TH ST, NW, WASHINGTON, DC 20036 USA VL - 230 ER - TY - JOUR T1 - Growth and population dynamics of anaerobic methane-oxidizing archaea and sulfate-reducing bacteria in a continuous-flow bioreactor JF - Applied and environmental microbiology Y1 - 2005 A1 - Girguis, Peter R. A1 - Cozen, Aaron E A1 - DeLong, Edward F PB - American Society for Microbiology VL - 71 IS - 7 ER - TY - JOUR T1 - Using electrochemical methods to study redox processes and harvest energy from marine sediments JF - Geochim Cosmochim Acta Y1 - 2005 A1 - Reimers, C A1 - Girguis, P. A1 - Westall, J A1 - Newman, D A1 - Stecher, H A1 - Howell, K A1 - Alleau, Y. VL - 69 ER - TY - JOUR T1 - Growth and population dynamics of anaerobic methane-oxidizing archaea and sulfate-reducing bacteria in a continuous-flow bioreactor JF - Applied and Environmental Microbiology Y1 - 2005 A1 - Girguis, P. R. A1 - Cozen, A. E. A1 - Delong, E. F. KW - anoxic marine-sediments KW - carbon isotopic evidence KW - communities KW - consortium KW - consumption KW - Ecology KW - identification KW - oxidation rates KW - reduction KW - sea-floor AB -

The consumption of methane in anoxic marine sediments is a biogeochemical phenomenon mediated by two archaeal groups (ANME-1 and ANME-2) that exist syntrophically with sulfate-reducing bacteria. These anaerobic methanotrophs have yet to be recovered in pure culture, and key aspects of their ecology and physiology remain poorly understood. To characterize the growth and physiology of these anaerobic methanotrophs and the syntrophic sulfate-reducing bacteria, we incubated marine sediments using an anoxic, continuous-How bioreactor during two experiments at different advective porewater flow rates. We examined the growth kinetics of anaerobic methanotrophs and Desulfosarcina-like sulfate-reducing bacteria using quantitative PCR as a proxy for cell counts, and measured methane oxidation rates using membrane-inlet mass spectrometry. Our data show that the specific growth rates of ANME-1 and ANME-2 archaea differed in response to porewater How rates. ANME-2 methanotrophs had the highest rates in lower-flow regimes (mu(ANME-2) 0.167 center dot week(-1)), whereas ANME-1 methanotrophs had the highest rates in higher-flow regimes (mu(ANME-1) = 0.218 center dot week(-1)). In both incubations, Desulfosarcina-like sulfate-reducing bacterial growth rates were approximately 0.3 center dot week(-1), and their growth dynamics suggested that sulfate-reducing bacterial growth might be facilitated by, but not dependent upon, an established anaerobic methanotrophic population. ANME-1 growth rates corroborate field observations that ANME-1 archaea flourish in higher-flow regimes. Our growth and methane oxidation rates jointly demonstrate that anaerobic methanotrophs are capable of attaining substantial growth over a range of environmental conditions used in these experiments, including relatively low methane partial pressures.

VL - 71 SN - 0099-2240 IS - 7 N1 -

944RETimes Cited:78Cited References Count:41

JO - Appl Environ MicrobAppl Environ Microb ER - TY - CHAP T1 - On the edge of a deep biosphere: Real animals in extreme environments T2 - The Subseafloor Biosphere at Mid-Ocean Ridges, 2004 Y1 - 2004 A1 - Childress, James J. A1 - Fisher, Charles F A1 - Felbeck, Horst A1 - Peter Girguis JF - The Subseafloor Biosphere at Mid-Ocean Ridges, 2004 PB - Blackwell Publishing Ltd ER - TY - CONF T1 - Towards Determining the Upper Temperature Limits to Life on Earth: An In-situ Sulfide-Microbial Incubator T2 - AGU Fall Meeting Abstracts Y1 - 2004 A1 - Kelley, D A1 - Baross, J A1 - Delaney, J. A1 - Girguis, P. A1 - Schrenk, M JF - AGU Fall Meeting Abstracts VL - 2004 ER - TY - CHAP T1 - On the edge of a deep biosphere: Real animals in extreme environments T2 - The Subseafloor Biosphere at Mid-Ocean Ridges, 2004 Y1 - 2004 A1 - Childress, James J. A1 - Fisher, Charles F A1 - Felbeck, Horst A1 - Peter Girguis JF - The Subseafloor Biosphere at Mid-Ocean Ridges, 2004 PB - Blackwell Publishing Ltd ER - TY - CONF T1 - Towards Determining the Upper Temperature Limits to Life on Earth: An In-situ Sulfide-Microbial Incubator T2 - AGU Fall Meeting Abstracts Y1 - 2004 A1 - Kelley, D A1 - Baross, J A1 - Delaney, J. A1 - Girguis, P. A1 - Schrenk, M JF - AGU Fall Meeting Abstracts VL - 2004 ER - TY - JOUR T1 - Growth and methane oxidation rates of anaerobic methanotrophic archaea in a continuous-flow bioreactor JF - Applied and Environmental Microbiology Y1 - 2003 A1 - Girguis, Peter R. A1 - Orphan, Victoria J A1 - Hallam, Steven J A1 - DeLong, Edward F PB - American Society for Microbiology VL - 69 IS - 9 ER - TY - JOUR T1 - Identification of methyl coenzyme M reductase A (mcrA) genes associated with methane-oxidizing archaea JF - Applied and environmental microbiology Y1 - 2003 A1 - Hallam, Steven J A1 - Girguis, Peter R. A1 - Preston, Christina M A1 - Richardson, Paul M A1 - DeLong, Edward F PB - American Society for Microbiology VL - 69 IS - 9 ER - TY - JOUR T1 - Growth and methane oxidation rates of anaerobic methanotrophic archaea in a continuous-flow bioreactor JF - Applied and Environmental Microbiology Y1 - 2003 A1 - Girguis, P. R. A1 - Orphan, V. J. A1 - Hallam, S. J. A1 - Delong, E. F. KW - anoxic marine-sediments KW - cells KW - cold seeps KW - consortium KW - consumption KW - diversity KW - identification KW - monterey bay KW - sulfate reduction KW - water chemistry AB -

Anaerobic methanotrophic archaea have recently been identified in anoxic marine sediments, but have not yet been recovered in pure culture. Physiological studies on freshly collected samples containing archaea and their sulfate-reducing syntrophic partners have been conducted, but sample availability and viability can limit the scope of these experiments. To better study microbial anaerobic methane oxidation, we developed a novel continuous-flow anaerobic methane incubation system (AMIS) that simulates the majority of in situ conditions and supports the metabolism and growth of anaerobic methanotrophic archaea. We incubated sediments collected from within and outside a methane cold seep in Monterey Canyon, Calif., for 24 weeks on the AMIS system. Anaerobic methane oxidation was measured in all sediments after incubation on AMIS, and quantitative molecular techniques verified the increases in methane-oxidizing archaeal populations in both seep and nonseep sediments. Our results demonstrate that the AMIS system stimulated the maintenance and growth of anaerobic methanotrophic archaea, and possibly their syntrophic, sulfate-reducing partners. Our data demonstrate the utility of combining physiological and molecular techniques to quantify the growth and metabolic activity of anaerobic microbial consortia. Further experiments with the AMIS system should provide a better understanding of the biological mechanisms of methane oxidation in anoxic marine environments. The AMIS may also enable the enrichment, purification, and isolation of methanotrophic archaea as pure cultures or defined syntrophic consortia.

VL - 69 SN - 0099-2240 IS - 9 N1 -

723MWTimes Cited:74Cited References Count:39

JO - Appl Environ MicrobAppl Environ Microb ER - TY - JOUR T1 - Identification of methyl coenzyme M reductase A (mcrA) genes associated with methane-oxidizing archaea JF - Applied and Environmental Microbiology Y1 - 2003 A1 - Hallam, S. J. A1 - Girguis, P. R. A1 - Preston, C. M. A1 - Richardson, P. M. A1 - Delong, E. F. KW - anoxic marine-sediments KW - Bacteria KW - Biochemistry KW - consumption KW - environments KW - methanogenesis KW - oxidation KW - pcr amplification KW - phylogenetic analysis KW - ribosomal-rna AB -

Phylogenetic and stable-isotope analyses implicated two methanogen-like archaeal groups, ANME-1 and ANME-2, as key participants in the process of anaerobic methane oxidation. Although nothing is known about anaerobic methane oxidation at the molecular level, the evolutionary relationship between methane-oxidizing archaea (MOA) and methanogenic archaea raises the possibility that MOA have co-opted key elements of the methanogenic pathway, reversing many of its steps to oxidize methane anaerobically. In order to explore this hypothesis, the existence and genomic conservation of methyl coenzyme M reductase (MCR), the enzyme catalyzing the terminal step in methanogenesis, was studied in ANME-1 and ANME-2 archaea isolated from various marine environments. Clone libraries targeting a conserved region of the alpha subunit of MCR (mcrA) were generated and compared from environmental samples, laboratory-incubated microcosms, and fosmid libraries. Four out of five novel mcrA types identified from these sources were associated with ANME-1 or ANME-2 group members. Assignment of mcrA types to specific phylogenetic groups was based on environmental clone recoveries, selective enrichment of specific MOA and mcrA types in a microcosm, phylogenetic congruence between mcrA and small-subunit rRNA tree topologies, and genomic context derived from fosmid sequences. Analysis of the ANME-1 and ANME-2 mcrA sequences suggested the potential for catalytic activity based on conservation of active-site amino acids. These results provide a basis for identifying methanotrophic archaea with mcrA sequences and define a functional genomic link between methanogenic and methanotrophic archaea.

VL - 69 SN - 0099-2240 N1 -

723MWTimes Cited:166Cited References Count:36

JO - Appl Environ MicrobAppl Environ Microb ER - TY - CONF T1 - Anaerobic enrichment of methane-oxidizing archaeal/bacterial consortia in deep-sea marine sediments T2 - General Meeting of the American Society for Microbiology Y1 - 2002 A1 - Girguis, P. R. A1 - Delong, E. F. JF - General Meeting of the American Society for Microbiology VL - 102 ER - TY - CONF T1 - Connecting genomics and biogeochemistry via the carbon-isotopic composition of ribosomal RNA T2 - AGU Fall Meeting Abstracts Y1 - 2002 A1 - Sessions, AL A1 - Pearson, A A1 - Hayes, J. M. A1 - Delong, E. F. A1 - Girguis, P. JF - AGU Fall Meeting Abstracts VL - 2002 ER - TY - CONF T1 - Anaerobic enrichment of methane-oxidizing archaeal/bacterial consortia in deep-sea marine sediments T2 - General Meeting of the American Society for Microbiology Y1 - 2002 A1 - Girguis, P. R. A1 - Delong, E. F. JF - General Meeting of the American Society for Microbiology VL - 102 ER - TY - CONF T1 - Connecting genomics and biogeochemistry via the carbon-isotopic composition of ribosomal RNA T2 - AGU Fall Meeting Abstracts Y1 - 2002 A1 - Sessions, AL A1 - Pearson, A A1 - Hayes, J. M. A1 - Delong, E. F. A1 - Girguis, P. JF - AGU Fall Meeting Abstracts VL - 2002 ER - TY - JOUR T1 - Effects of metabolite uptake on proton-equivalent elimination by two species of deep-sea vestimentiferan tubeworm, Riftia pachyptila and Lamellibrachia cf luymesi: proton elimination is a necessary adaptation to sulfide-oxidizing chemoautotrophic symbiont JF - Journal of experimental biology Y1 - 2002 A1 - Girguis, P. R. A1 - Childress, J. J. A1 - Freytag, J. K. A1 - Klose, K. A1 - Stuber, R. PB - Company of Biologists VL - 205 IS - 19 ER - TY - JOUR T1 - Effects of metabolite uptake on proton-equivalent elimination by two species of deep-sea vestimentiferan tubeworm, Riftia pachyptila and Lamellibrachia cf luymesi: proton elimination is a necessary adaptation to sulfide-oxidizing chemoautotrophic symbiont JF - Journal of Experimental Biology Y1 - 2002 A1 - Girguis, P. R. A1 - Childress, J. J. A1 - Freytag, J. K. A1 - Klose, K. A1 - Stuber, R. KW - acquisition KW - bacterial symbiont KW - Biochemistry KW - carbon-dioxide KW - chemoautotrophy KW - hydrothermal vent KW - hydrothermal vent tubeworm KW - ion-transport KW - jones KW - lamellibrachia cf luymesi KW - metabolite uptake KW - oxidation KW - physiology KW - proton-equivalent KW - riftia pachyptila KW - sensitivity KW - sulfide KW - symbiosis KW - tubeworm KW - urechis caupo KW - vestimentiferan KW - worm AB -

Intracellular symbiosis requires that the host satisfy the symbiont's metabolic requirements, including the elimination of waste products. The hydrothermal vent tubeworm Riftia pachyptila and the hydrocarbon seep worm Lamellibrachia cf luymesi are symbiotic with chemolithoautotrophic bacteria that produce sulfate and protons as end-products. In this report, we examine the relationship between symbiont metabolism and host proton equivalent elimination in R. pachyptila and L cf luymesi, and the effects of sulfide exposure on proton-equivalent elimination by Urechis caupo, an echiuran worm that lacks intracellular symbionts (for brevity, we will hereafter refer to proton-equivalent elimination as 'proton elimination'). Proton elimination by R. pachyptila and L. cf luymesi constitutes the worms' largest mass-specific metabolite flux, and R. pachyptila proton elimination is, to our knowledge, the most rapid reported for any metazoan. Proton elimination rates by R. pachyptila and L. cf luymesi correlated primarily with the rate of sulfide oxidation. Prolonged exposure to low environmental oxygen concentrations completely inhibited the majority of proton elimination by R. pachyptila, demonstrating that proton elimination does not result primarily from anaerobic metabolism. Large and rapid increases in environmental inorganic carbon concentrations led to short-lived proton elimination by R. pachyptila, as a result of the equilibration between internal and external inorganic carbon pools. U. caupo consistently exhibited proton elimination rates 5-20 times lower than those, of L cf luymesi and R. pachyptila upon exposure to sulfide. Treatment with specific ATPase inhibitors completely inhibited a fraction of proton elimination and sulfide and inorganic carbon uptake by R. pachyptila, suggesting that proton elimination occurs in large part via K+/H+-ATPases and Na+/H+-ATPases. In the light of these results, we suggest that protons are the primary waste product of the symbioses of R. pachyptila and L cf luymesi, and that proton elimination is driven by symbiont metabolism, and may be the largest energetic cost incurred by the worms.

VL - 205 SN - 0022-0949 N1 -

604ZZTimes Cited:28Cited References Count:38

ER - TY - JOUR T1 - A paradox resolved: sulfide acquisition by roots of seep tubeworms sustains net chemoautotrophy JF - Proceedings of the National Academy of Sciences Y1 - 2001 A1 - Freytag, John K A1 - Girguis, Peter R. A1 - Bergquist, Derk C A1 - Andras, Jason P A1 - Childress, James J. A1 - Fisher, Charles R PB - National Academy of Sciences VL - 98 IS - 23 ER - TY - JOUR T1 - A paradox resolved: Sulfide acquisition by roots of seep tubeworms sustains net chemoautotrophy. JF - Proceedings of the National Academy of Sciences of the United States of America Y1 - 2001 A1 - Freytag, J. K. A1 - Girguis, P. R. A1 - Bergquist, D. C. A1 - Andras, J. P. A1 - Childress, J. J. A1 - Fisher, C. R. KW - biology KW - calyptogena-magnifica KW - communities KW - gulf KW - hydrothermal vent KW - mexico KW - organisms KW - symbioses KW - transport KW - worm riftia-pachyptila AB -

Vestimentiferan tubeworms, symbiotic with sulfur-oxidizing chemoautotrophic bacteria, dominate many cold-seep sites in the Gulf of Mexico. The most abundant vestimentiferan species at these sites, Lamellibrachia cf. luymesi, grows quite slowly to lengths exceeding 2 meters and lives in excess of 170-250 years. L. cf. luymesi can grow a posterior extension of its tube and tissue, termed a "root," down into sulfidic sediments below its point of original attachment. This extension can be longer than the anterior portion of the animal. Here we show, using methods optimized for detection of hydrogen sulfide down to 0.1 muM in seawater, that hydrogen sulfide was never detected around the plumes of large cold-seep vestimentiferans and rarely detectable only around the bases of mature aggregations. Respiration experiments, which exposed the root portions of L. cf. luymesi to sulfide concentrations between 51-561 muM, demonstrate that L. cf. luymesi use their roots as a respiratory surface to acquire sulfide at an average rate of 4.1 mu mol.g(-1).h(-1). Net dissolved inorganic carbon uptake across the plume of the tubeworms was shown to occur in response to exposure of the posterior (root) portion of the worms to sulfide, demonstrating that sulfide acquisition by roots of the seep vestimentiferan L. cf. luymesi can be sufficient to fuel net autotrophic total dissolved inorganic carbon uptake.

VL - 98 SN - 0027-8424 IS - 23 N1 -

490WMTimes Cited:82Cited References Count:37

JO - P Natl Acad Sci USAP Natl Acad Sci USA ER - TY - THES T1 - Metabolite flux of the tubeworm Riftia pachyptila: Stoichiometric balance of the chemoautotrophic symbiosis and the potential rates of primary production Y1 - 2000 A1 - Girguis, Peter Riad PB - University of California, Santa Barbara ER - TY - JOUR T1 - Fate of nitrate acquired by the tubeworm Riftia pachyptila JF - Applied and environmental microbiology Y1 - 2000 A1 - Girguis, Peter R. A1 - Lee, Raymond W A1 - Desaulniers, Nicole A1 - Childress, James J. A1 - Pospesel, Mark A1 - Felbeck, Horst A1 - Zal, Franck PB - American Society for Microbiology VL - 66 IS - 7 ER - TY - THES T1 - Metabolite flux of the tubeworm Riftia pachyptila: Stoichiometric balance of the chemoautotrophic symbiosis and the potential rates of primary production Y1 - 2000 A1 - Girguis, Peter Riad PB - University of California, Santa Barbara ER - TY - JOUR T1 - A paradox resolved: sulfide acquisition by roots of seep tubeworms sustains net chemoautotrophy JF - Comparative Biochemistry and Physiology–Part B: Biochemistry and Molecular Biology Y1 - 2000 A1 - Freytag, John K A1 - Girguis, Peter R. A1 - Bergquist, Derk C A1 - Andras, Jason P A1 - Childress, James J. A1 - Fisher, Charles R IS - 126 ER - TY - JOUR T1 - Fate of nitrate acquired by the tubeworm Riftia pachyptila. JF - Applied and Environmental Microbiology Y1 - 2000 A1 - Girguis, P. R. A1 - Lee, R. W. A1 - Desaulniers, N. A1 - Childress, J. J. A1 - Pospesel, M. A1 - Felbeck, H. A1 - Zal, F. KW - assimilation KW - blood KW - chemoautotrophic symbionts KW - hydrothermal vent animals KW - inorganic nitrogen KW - Respiration KW - rose garden KW - sulfide KW - uric-acid KW - worm AB -

The hydrothermal vent tubeworm Riftia pachyptila lacks a mouth and gut and lives in association with intracellular, sulfide-oxidizing chemoautotrophic bacteria. Growth of this tubeworm requires an exogenous source of nitrogen for biosynthesis, and, as determined in previous studies, environmental ammonia and free amino acids appear to be unlikely sources of nitrogen. Nitrate, however, is present in situ (K, Johnson, J. Childress, R. Hessler, C. Sakamoto-Arnold, and C. Beehler, Deep-Sea Res. 35:1723-1744, 1988), is taken up by the host, and can be chemically reduced by the symbionts (U. Hentschel and H. Felbeck, Nature 366:338-340, 1993), Here we report that at an in situ concentration of 40 mu M, nitrate is acquired by R, pachyptila at a rate of 3.54 mu mol g(-1) h(-1), while elimination of nitrite and elimination of ammonia occur at much lower rates (0.017 and 0.21 mu mol g(-1) h(-1), respectively), We also observed reduction of nitrite (and accordingly nitrate) to ammonia in the trophosome tissue. When R. pachyptila tubeworms are exposed to constant in situ conditions for 60 h, there is a difference between the amount of nitrogen acquired via nitrate uptake and the amount of nitrogen lost via nitrite and ammonia elimination, which indicates that there is a nitrogen "sink" Our results demonstrate that storage of nitrate does not account for the observed stoichiometric differences in the amounts of nitrogen, Nitrate uptake was not correlated with sulfide or inorganic carbon flux, suggesting that nitrate is probably not an important oxidant in metabolism of the symbionts, Accordingly, we describe a nitrogen flux model for this association, in which the product of symbiont nitrate reduction, ammonia, is the primary source of nitrogen for the host and the symbionts and fulfills the association's nitrogen needs via incorporation of ammonia into amino acids.

VL - 66 SN - 0099-2240 IS - 7 N1 -

332DETimes Cited:38Cited References Count:36

JO - Appl Environ MicrobAppl Environ Microb ER - TY - JOUR T1 - Physiological functioning of carbonic anhydrase in the hydrothermal vent tubeworm Riftia pachyptila JF - The Biological Bulletin Y1 - 1999 A1 - Goffredi, Shana K A1 - Girguis, Peter R. A1 - Childress, James J. A1 - Desaulniers, Nicole T PB - MBL VL - 196 IS - 3 ER - TY - JOUR T1 - Physiological functioning of carbonic anhydrase in the hydrothermal vent tubeworm Riftia pachyptila JF - Biological Bulletin Y1 - 1999 A1 - Goffredi, S. K. A1 - Girguis, P. R. A1 - Childress, J. J. A1 - Desaulniers, N. T. KW - blood KW - co2 excretion KW - facilitation KW - gill KW - inorganic carbon KW - jones KW - symbiont KW - transport KW - worm AB -

On the basis of our experiments, it is clear that carbonic anhydrase (CA) plays an important role in the CO2-concentrating mechanisms in Riftia pachyptila. Plume tissue from freshly collected animals had the highest CA activity, 253.7 +/- 36.0 mu mol CO2 min(-1) g(-1) wet wt, and trophosome activity averaged 109.4 +/- 17.9 mu mol CO2 min(-1) g(-1) wet wt. Exposure of living worms to ethoxyzolamide, a carbonic anhydrase inhibitor, resulted in a 99% decrease in CA activity (from 103.9 +/- 38.6 to 0.7 +/- 0.2 mu mol CO2 min(-1) g(-1) wet wt in the plume tissue and 57.6 +/- 17.9 to 0.03 +/- 0,11 mu mol CO2 min(-1) g(-1) wet wt in the trophosome) and essentially a complete cessation of Sigma CO2, uptake. High concentrations of CA appear to facilitate the equilibration between inorganic carbon (Ci) in the external and internal environments, greatly enhancing the diffusion of CO2 into the animal. In summary, R. pachyptila demonstrates very effective acquisition of inorganic carbon from the environment, thereby providing the symbionts with large amounts of CO2. This effective acquisition is made possible by three factors: extremely effective pH regulation, a large external pool of CO2, and, described in this paper, high levels of carbonic anhydrase.

VL - 196 SN - 0006-3185 N1 -

210KRTimes Cited:20Cited References Count:32

JO - Biol BullBiol Bull ER - TY - JOUR T1 - H+ equivalent elimination by the tubeworm Riftia pachyptila JF - Cah. Biol. Mar Y1 - 1998 A1 - Girguis, Peter R. A1 - Childress, James J. VL - 39 IS - 39 ER - TY - JOUR T1 - H+ equivalent elimination by the tubeworm Riftia pachyptila JF - Cah. Biol. Mar Y1 - 1998 A1 - Girguis, Peter R. A1 - Childress, James J. VL - 39 IS - 39 ER - TY - JOUR T1 - H+ equivalent elimination by the tube-worm Riftia pachyptila. JF - Cahiers de Biologie Marine Y1 - 1998 A1 - Girguis, P. R. A1 - Childress, J. J. KW - Annelids KW - Biochemistry KW - Invertebrates KW - Marine zones KW - Pacific Ocean VL - 39 JO - Cah Biol Mar ER -