@article {C5LC00978B,
	title = {Nanoporous microscale microbial incubators},
	journal = {Lab Chip},
	volume = {16},
	year = {2016},
	pages = {480-488},
	publisher = {The Royal Society of Chemistry},
	abstract = {Reconstruction of phylogenetic trees based on 16S rRNA gene sequencing reveals abundant microbial diversity that has not been cultured in the laboratory. Many attribute this so-called {\textquoteright}great plate count anomaly{\textquoteright} to traditional microbial cultivation techniques, which largely facilitate the growth of a single species. Yet, it is widely recognized that bacteria in nature exist in complex communities. One technique to increase the pool of cultivated bacterial species is to co-culture multiple species in a simulated natural environment. Here, we present nanoporous microscale microbial incubators (NMMI) that enable high-throughput screening and real-time observation of multi-species co-culture. The key innovation in NMMI is that they facilitate inter-species communication while maintaining physical isolation between species, which is ideal for genomic analysis. Co-culture of a quorum sensing pair demonstrates that the NMMI can be used to culture multiple species in chemical communication while monitoring the growth dynamics of individual species.},
	doi = {10.1039/C5LC00978B},
	url = {http://dx.doi.org/10.1039/C5LC00978B},
	author = {Ge, Zhifei and Peter R. Girguis and Buie, Cullen R.}
}
