@article {510986,
	title = {Characterizing Microbial Community and Geochemical Dynamics at Hydrothermal Vents Using Osmotically Driven Continuous Fluid Samplers},
	journal = {Environmental Science \& Technology},
	volume = {47},
	number = {9},
	year = {2013},
	note = {<p>141WATimes Cited:2Cited References Count:36</p>},
	month = {May 7},
	pages = {4399-4407},
	abstract = {<p>Microbes play a key role in mediating aquatic biogeochemical cycles. However, our understanding of the relationships between microbial phylogenetic/physiological diversity and habitat physicochemical characteristics is restrained by our limited capacity to concurrently collect microbial and geochemical samples at appropriate spatial and temporal scales. Accordingly, we have developed a low-cost, continuous fluid sampling system (the Biological OsmoSampling System, or BOSS) to address this limitation. The BOSS does not use electricity, can be deployed in harsh/remote environments, and collects/preserves samples with daily resolution for \&gt;1 year. Here, we present data on the efficacy of DNA and protein preservation during a 1.5 year laboratory study as well as the results of two field deployments at deep-sea hydrothermal vents, wherein we examined changes in microbial diversity, protein expression, and geochemistry over time. Our data reveal marked changes in microbial composition co-occurring with changes in hydrothermal fluid composition as well as the temporal dynamics of an enigmatic sulfide-oxidizing symbiont in its free-living state. We also present the first data on in situ protein preservation and expression dynamics highlighting the BOSS{\textquoteright}s potential utility in meta-proteomic studies. These data illustrate the value of using BOSS to study relationships among microbial and geochemical phenomena and environmental conditions.</p>},
	keywords = {bacterial, diversity, dominance, field, growth, high-resolution, mid-atlantic ridge, population-dynamics, quantification},
	isbn = {0013-936X},
	author = {Robidart, J. and Callister, S. J. and Song, P. F. and Nicora, C. D. and Wheat, C. G. and Girguis, P. R.}
}
